Conserved Protein Domain Family
nitrilases_CHs

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cd07564: nitrilases_CHs 
Nitrilases, cyanide hydratase (CH)s, and similar proteins (class 1 nitrilases)
Nitrilases (nitrile aminohydrolases, EC:3.5.5.1) hydrolyze nitriles (RCN) to ammonia and the corresponding carboxylic acid. Most nitrilases prefer aromatic nitriles, some prefer arylacetonitriles and others aliphatic nitriles. This group includes the nitrilase cyanide dihydratase (CDH), which hydrolyzes inorganic cyanide (HCN) to produce formate. It also includes cyanide hydratase (CH), which hydrolyzes HCN to formamide. This group includes four Arabidopsis thaliana nitrilases (Ath)NIT1-4. AthNIT1-3 have a strong substrate preference for phenylpropionitrile (PPN) and other nitriles which may originate from the breakdown of glucosinolates. The product of PPN hydrolysis, phenylacetic acid has auxin activity. AthNIT1-3 can also convert indoacetonitrile to indole-3-acetic acid (IAA, auxin), but with a lower affinity and velocity. From their expression patterns, it has been speculated that NIT3 may produce IAA during the early stages of germination, and that NIT3 may produce IAA during embryo development and maturation. AthNIT4 has a strong substrate specificity for the nitrile, beta-cyano-L-alanine (Ala(CN)), an intermediate of cyanide detoxification. AthNIT4 has both a nitrilase activity and a nitrile hydratase (NHase) activity, which generate aspartic acid and asparagine respectively from Ala(CN). NHase catalyzes the hydration of nitriles to their corresponding amides. This subgroup belongs to a larger nitrilase superfamily comprised of belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 1.
Statistics
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PSSM-Id: 143588
View PSSM: cd07564
Aligned: 107 rows
Threshold Bit Score: 297.862
Threshold Setting Gi: 46109632
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                #                                       
P82605         7 YRVAAVQASPVLLdLDATIDKTCRLVDEAaanGAKVIAFPEAFIPGYPWWiwlgna-----dygmkyYIQLYKNSVe--- 78  Bacillus sp. OxB-1
ABH04285      13 VKVAVIQASSVIMdRDATTKKAVSLIHQAaekGAKIVVFPEAFIPAYPRGlsfgttigsrsaegrkdWYRYWSNSVa--- 89  Geobacillus pal...
YP_001917244   7 VRTAVIQAAPIIMdREKTLNKALDLIKEAkneQAEIVVFPEAFIPAYPRGlsfgylvgsrtmegrndFLRYYKNAVp--- 83  Natranaerobius ...
ZP_01687274    4 LDIAVIQAAPVLFdLEQSLDKTYDLLKKAtaqGAKMVVFPESFLPAYPRGlsfgtvvgsrtdagrevWQLYWKNSVk--- 80  Microscilla mar...
ZP_02087592   20 CKIAVIQAAPVMFdKDACTQKAVDLIQEAsrrGAQLMVFPELFIPGYPYGmtfgfrvgsrsgegrqdWKLYLDNSIl--- 96  Clostridium bol...
EEO36031       8 CKIAVVQAAPILFnKDECVKKVIDYIEESaskGSELIVFPELFIPGYPYGmtfgftvgsrkelgrvdWKRYYDNSIv--- 84  Fusobacterium m...
ZP_01719620    3 VKVCLVQESPVFFdKEKTLQKLERLCEKYakeGNQLIVFPESFIPGYPRGfsfgakigsrtpegrdlYEEYYQNSFd--- 79  Algoriphagus sp...
Q23384         2 PKIAIVQAGTPLFdKPATLEKVKKNVEEAagnGAELVLFPEAFIGGYPKWnsfgitmgtrtpegrkeFKRYFENAIe--- 78  Caenorhabditis ...
XP_381874      7 IRLGTASPGTASS-TPETIALIDTLAQRAassHIDILLLPEAFIGGYPRGssfgckigdrsaagrdeFARYFDQAIdlgd 85  Gibberella zeae...
EER30010       5 LKVAVSQARTRDT-LQQTLEALERITRVAasrGARIILFPEGYLGGYPRTctwgaamggrddsgreqYLHYYQAAVdmgd 83  Coccidioides po...
Feature 1                                                                                       #
P82605        79 ------------------------IPSLAVQKLSSAGTN-KVYFCVSVTEKD----GGSLYLTQLWF-DPNGdLIGKHRK 128 Bacillus sp. OxB-1
ABH04285      90 ------------------------VPDETTEQLGEAARKaGVYLVIGVTERDnefsGGTLYCSVLFF-DSDGqLLGKHRK 144 Geobacillus pal...
YP_001917244  84 ------------------------VPGDTTEKLGEAARKaGIYLVIGVTEIDteknNNTLYCTILYF-GPDGrLLDKHQK 138 Natranaerobius ...
ZP_01687274   81 ------------------------VPGKATNLLAKWAKEyAVYLVMGITEQDt--vNGSLYCSLLYF-SPEGhLLGKHRK 133 Microscilla mar...
ZP_02087592   97 ------------------------VPGKETEKIGMAAREaGAYVSIGVSERDg--vTGTLYNTNLFF-SPRGsLVCVHRK 149 Clostridium bol...
EEO36031      85 ------------------------IPGKETDAIAEVVKKyKVYVSIGVSERDl--eTATLYNSNIIF-SPEGeIVSVHRK 137 Fusobacterium m...
ZP_01719620   80 ------------------------LDSEDLTRLEKLCHQlNIYLVCGVTERMk--qHGSLYCSMIYI-SPKG-LLGVHRK 131 Algoriphagus sp...
Q23384        79 ------------------------ENGEESKLIESLAAQnNIHIVIGVVERE----ASTLYCSVFFY-SPSG-YLGKHRK 128 Caenorhabditis ...
XP_381874     86 tvgpagggagikwvkrqigeedevRGDGSREELERIARDsGVFVVVGCIEKA----GGSLYCSVVYVcPKEG-MLGKRRK 160 Gibberella zeae...
EER30010      84 tpagagddw--vsrrlpiaegknyRGDGTREVLERIAREtDMLIIVGLIERC----AGSLYCAVVYV-DPKRgVLGKRRK 156 Coccidioides po...
Feature 1           #  #                              ## ##                   #                  
P82605       129 LKATNAEKTIWGDGDGSMMPVFET----EFGNLGGLQCWEHFLPLNVAAMasMNEQVHVASWpigmpqeghlfgpEQCVT 204 Bacillus sp. OxB-1
ABH04285     145 LKPTAAERIVWGEGDGSTLPVFDT----PYGRIGALICWENYMPLARAAMyaQGIQIYIAPTad---------arETWQS 211 Geobacillus pal...
YP_001917244 139 LKPTGSERLIWGEGDGSTLSVVNT----PHGKLGGLICWENYMPLARTAMyqKGVSLYVAPTad---------arSVWQS 205 Natranaerobius ...
ZP_01687274  134 LKPTAAERIIWGEGDATTLQTYPT----PYGNIGGLICWENYMPLARMALyqQGIHLYLAPTad---------arESWQA 200 Microscilla mar...
ZP_02087592  150 LKPTGAERVVWGDADRGYFPVEDT----PWGVMGSLICWESYMPLARAALyeKGVALYISPNtn---------dnPEWQS 216 Clostridium bol...
EEO36031     138 LKPTGSERVVWGDANKYYFPVCDT----PWGNIGSLICWESYMPLARVALyeKGITIYISPNtn---------dnKEWQD 204 Fusobacterium m...
ZP_01719620  132 IKPTGIERLVWAEASGDSLVTFDT----KIGKLGGLICWENYMPLARMAMysQGVEIYIAPTad---------arESWVD 198 Algoriphagus sp...
Q23384       129 LLPTALERCVWGQGDGSTMPVFST----SVGKIGSAICWENYMPLYRMTLysKEIQIYLAPTvd---------drDVWLS 195 Caenorhabditis ...
XP_381874    161 VLPTGTERLIWAQGSPSTLRAVTTvirgIRINLAAAICWESYMPLLRQSLysQNINLYLAPTad---------frDAWLS 231 Gibberella zeae...
EER30010     157 VMPTGTERLVWAQGSASTLKAVTTevdgVKLTLAAAICWENYMPLLRQSLyqQNVNLYLAPTad---------grDTWLP 227 Coccidioides po...
Feature 1                                                                                        
P82605       205 ATKYYAISNQVFCLLSSQIWTEEqrdkicetee------qrnfmkvghGFSKIIAPnGMEIGNKLa----hDEEGITYAD 274 Bacillus sp. OxB-1
ABH04285     212 TIRHIALEGRCFVLSANQYVTKDmypkdlacyde----lasspeimsrGGSAIVGPlGEYVAEPVf-----GKEDIIIAE 282 Geobacillus pal...
YP_001917244 206 TIRHIALEGRCYVLSCNQFVTKDmypddleyyhe----lqnqpnimcsGGSAIVNPmGEYVVEPVy-----NKEEMLLAD 276 Natranaerobius ...
ZP_01687274  201 TMQHIALEGRCFVVGCNQFVTKSmypphlrelpe----mtsqpevmsrGGSVVLSPlGKVLAGPVf-----DREEVLLAT 271 Microscilla mar...
ZP_02087592  217 TVQHIALEGRCYFINCNMYITRDmypenlccrde----idglpdtvcrGGSCIVDPyGHYVTEPVw-----DKEAVIYAD 287 Clostridium bol...
EEO36031     205 TIKHIAIEGHCYFINCDMYFTKDdypkdllskee----idrldnivcrGGSCIVDPyGHYETEPVw-----DREEIIYAE 275 Fusobacterium m...
ZP_01719620  199 TMRHIAIEGRCFVLACNQYFTKSmyphrlqkdm------eeveedfckGGTVIFSPlGELIAGPLy-----GEAGALSME 267 Algoriphagus sp...
Q23384       196 TMRTIALEGRCFVVSACQFLKSSdypldhplrk-----ehgedkvlirGGSCAVDPlGTVLVEPDf-----TKETIRYTE 265 Caenorhabditis ...
XP_381874    232 LARTIGVEGRCFVVCSNMCVPKDasaetngng--------ggpavrerRGSCLTDE-GFEIALPKsp---tRSRRRRKSV 299 Gibberella zeae...
EER30010     228 LMQTVAFEGRAVVLTCNQCVRKSqlpswvkgddnaqkdssdpdpiltgGGSCIISPmGKVLAGPVwnvdddDEEGLQISE 307 Coccidioides po...
Feature 1                                             
P82605       275 IDLE--QIIPGKf----lIDSAGHYSTPGFLSLSFDR 305 Bacillus sp. OxB-1
ABH04285     283 LDMK--QIAYSQf----dFDPVGHYARPDVFKLLVNK 313 Geobacillus pallidus
YP_001917244 277 LNMD--LVIKGKm----dFDVVGHYDRTDVFQLKINE 307 Natranaerobius thermophilus JW/NM-WN-LF
ZP_01687274  272 LDLD--DIIKSKl----dFDPVGHYSRPDMLNFNISN 302 Microscilla marina ATCC 23134
ZP_02087592  288 LEMD--RVSASRm----eFDVCGHYSRPDVLRLQIDD 318 Clostridium bolteae ATCC BAA-613
EEO36031     276 LDMN--KVPMSRm----eFDVCGHYSRPDVLQLKVDD 306 Fusobacterium mortiferum ATCC 9817
ZP_01719620  268 IDLN--LITKSKl----dFDPIGHYARDDIFEFKVKG 298 Algoriphagus sp. PR1
Q23384       266 FDLS--DLALGKm----dLDVVGHYSRPDVFQLKVNE 296 Caenorhabditis elegans
XP_381874    300 FDEDgnEIVLGCeddgeaPPPPPPAARPQVAALKTSG 336 Gibberella zeae PH-1
EER30010     308 ADFE--DCVRGRl----dLDVAGSYSRNDAFKLTVEG 338 Coccidioides posadasii C735 delta SOWgp

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