Conserved Protein Domain Family
Peptidases_S8_Lantibiotic_specific_protease

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cd07482: Peptidases_S8_Lantibiotic_specific_protease 
Peptidase S8 family domain in Lantiobiotic (lanthionine-containing antibiotics) specific proteases
Lantiobiotic (lanthionine-containing antibiotics) specific proteases are very similar in structure to serine proteases. Lantibiotics are ribosomally synthesised antimicrobial agents derived from ribosomally synthesised peptides with antimicrobial activities against Gram-positive bacteria. The proteases that cleave the N-terminal leader peptides from lantiobiotics include: epiP, nsuP, mutP, and nisP. EpiP, from Staphylococcus, is thought to cleave matured epidermin. NsuP, a dehydratase from Streptococcus and NisP, a membrane-anchored subtilisin-like serine protease from Lactococcus cleave nisin. MutP is highly similar to epiP and nisP and is thought to process the prepeptide mutacin III of S. mutans. Members of the peptidases S8 (subtilisin and kexin) and S53 (sedolisin) clan include endopeptidases and exopeptidases. The S8 family has an Asp/His/Ser catalytic triad similar to that found in trypsin-like proteases, but do not share their three-dimensional structure and are not homologous to trypsin. Serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base. The S53 family contains a catalytic triad Glu/Asp/Ser with an additional acidic residue Asp in the oxyanion hole, similar to that of subtilisin. The serine residue here is the nucleophilic equivalent of the serine residue in the S8 family, while glutamic acid has the same role here as the histidine base. However, the aspartic acid residue that acts as an electrophile is quite different. In S53 the it follows glutamic acid, while in S8 it precedes histidine. The stability of these enzymes may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. There is a great diversity in the characteristics of their members: some contain disulfide bonds, some are intracellular while others are extracellular, some function at extreme temperatures, and others at high or low pH values.
Statistics
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PSSM-Id: 173808
View PSSM: cd07482
Aligned: 14 rows
Threshold Bit Score: 255.368
Threshold Setting Gi: 114047125
Created: 11-Dec-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitecatalytic triad
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               #                                                                        
P30199       142 KAKVALVDSGVNSSHTDLk-----sINKIVNev----pKNGFRgsendesgn---------------------------- 184 Staphylococcus ...
ABA00872     146 KATIGVIDSGITYDNPDIfs----hIISVQNfts--nlETGVVdkqnv-------------------------------- 187 Streptococcus u...
NP_646578    133 NTKIAIIDTGVMKNHDDLkn----nFSTDSKnl---vpLNGFRgtepeetgd---------------------------- 177 Staphylococcus ...
Q07596       252 KISVGIIDSGIMEEHPDLsn----sLGNYFKnl---vpKGGFDneepdetgn---------------------------- 296 Lactococcus lac...
NP_359363    172 KVSIAILDSGVDLQNTGLlk----nLSNHSKny---vpNKGYLgkeegeegi---------------------------- 216 Streptococcus p...
AAD56147     131 NVTVALVDSGIDVDHNAFtg----mIDSRSKnf---vpAGGYDnsessetgs---------------------------- 175 Streptococcus m...
ZP_02613679  158 NVVIAVLDSGIDKDHPDLkn----nLLGGENfi---pkDFNNDkteigdp------------------------------ 200 Clostridium bot...
ZP_02326316   39 EIKIAIIDSGVDRNHPDLah----sIVSEGKsfi--ddMPATVdl----------------------------------- 77  Paenibacillus l...
YP_869253    166 KARVAVLDSGYFPHEDMEtp---keQAKFIKsamyyfpEGNYAynlalncltqdptnsgldatcdasnivtlgdqtnnal 242 Shewanella sp. ...
YP_001918837 143 SVTVGVIDTGIDADHPDLkenlefaTVHYEDae---eeDAYVDp------------------------------------ 183 Natranaerobius ...
Feature 1                                     #   #                                              
P30199       185 --------------------knfeedklNHGTLVAGQIGangnlk------gvnpgvEMNVYRVFgskks--emlwVSKG 236 Staphylococcus ...
ABA00872     188 ------------------------vdktGHATSVVGQISssgeyl------giapemKIRMYRVFdegna--qdqwILKA 235 Streptococcus u...
NP_646578    178 --------------------vhdvndrkGHGTMVSGQTSangkli------gvapnnKFTMYRVFgskkt--ellwVSKA 229 Staphylococcus ...
Q07596       297 --------------------psdivdkmGHGTEVAGQITangnil------gvapgiTVNIYRVFgenls--ksewVARA 348 Lactococcus lac...
NP_359363    217 --------------------isdiqdrlGHGTAVVAQIVgddnin------gvnphvNINVYRIFgkssa--spdwIVKA 268 Streptococcus p...
AAD56147     176 --------------------indiddkkGHGTAVAGQIAangqif------gvspgtNLLVYRVFgksks--kecwILKA 227 Streptococcus m...
ZP_02613679  201 ---------------------rdiedrlGHGTCISGSIAgngrik------giapniGFKSYRVFdsrgh-tnatiMSSA 252 Clostridium bot...
ZP_02326316   78 ---------------------------sGHGTMMAGAIAargkll------gigpglGIVPYKIIqtnns-ghssdVIKA 123 Paenibacillus l...
YP_869253    243 dkswkpskfvyneqtklndpteykicinGHGLSVASSIAaiqnngkgiasavgseniDIVPVKVIdsctgsaltsdLIKA 322 Shewanella sp. ...
YP_001918837 184 ---------------------------nGHGTHVAGSIAadgevk------gvgpdlGLASFRVMndegv-ipsvaTADA 229 Natranaerobius ...
Feature 1                                  ###                                               # ##
P30199       237 IIDAANdd------------nDVINVSLGnylikdnqnkkk-------lrddekvdydalQKAINYAQkkgSIVVAAVGN 297 Staphylococcus ...
ABA00872     236 LIQAAKdd------------vDVINLSLGeyllrgstde-----------ddntaliniyQRAINYAHnqgSVVVASVGD 292 Streptococcus u...
NP_646578    230 IVQAANdg------------nQVINISVGsyiildkndhqt-------frkdekveydalQKAINYAKkkkSIVVAAAGN 290 Staphylococcus ...
Q07596       349 IRRAADdg------------nKVINISAGqylmisgsydd---------gtndyqeylnyKSAINYATakgSIVVAALGN 407 Lactococcus lac...
NP_359363    269 IFDAVDdg------------nDIINLSTGqylmidgeyed---------gtndfetflkyKKAIDYANqkgVIIVAALGN 327 Streptococcus p...
AAD56147     228 IIDATNng------------aNVINLSLGqyikipngdi------------wesaealryKFAIDYATrhnVIVVAATGN 283 Streptococcus m...
ZP_02613679  253 IIKATDdg------------aNVINLSMAgydlkgkcywtdpdtgvkhdlgddmaeyelyKRAIQYALnhnVTVVTAAGN 320 Clostridium bot...
ZP_02326316  124 IDLAIDdr------------nDIINLSLGsykstiges--------------daglireyNQIIRKAAskgIIVVGSMGT 177 Paenibacillus l...
YP_869253    323 IYWAAKsddtfeglepisepvDVINLSLGsnknelc-----------------evgynafADAVDYAKqkgIVVVAALGN 385 Shewanella sp. ...
YP_001918837 230 IATAADkg------------vDVVNISLGalrditdee--------------srelyhmtRRVVEYADdqgMIMAVSAGN 283 Natranaerobius ...
Feature 1                                                                                        
P30199       298 Dginvkkvkeinkkr-----nlnsktskkvydsPANLNNVMTVGSIDDNDYIs---eFSNYGNNFIDLMTIGGsyklldK 369 Staphylococcus ...
ABA00872     293 EgidlqnqdklktylgglrgkdysqvngtvqdiPAELDNVVTVGSVDDNDLVs---sFSNKGKDSIDIYATGGgsrelsQ 369 Streptococcus u...
NP_646578    291 Dgidvndkqklklq-------reyqgngevkdvPASMDNVVTVGSTDQKSNLs---eFSNFGMNYTDIAAPGGsfaylnQ 360 Staphylococcus ...
Q07596       408 Dslniqdnqtminflk---rfrsikvpgkvvdaPSVFEDVIAVGGIDGYGNIs----DFSNIGADAIYAPAGTtan-fkK 479 Lactococcus lac...
NP_359363    328 Dslnvsnqsdllklis---srkkvrkpglvvdvPSYFSSTISVGGIDRLGNLs---dFSNKGDSDAIYAPAGStls-lsE 400 Streptococcus p...
AAD56147     284 Dglsddngevktyynsq-hsgqdmsqndtvedyPSVLPNAIAVGSSDNNNQRssfsnYYNQYQDNFILAPGGGtpl-ldQ 361 Streptococcus m...
ZP_02613679  321 DgldccntkslteflnnrdkedgfsyegvtyeiPGSIDGVINVSATGKNDKIa---tYSNYGKNFIDVAAPGGdhskteS 397 Clostridium bot...
ZP_02326316  178 Hgmflskk-------------------seychvPGGLSHVISVAATNKEGMLa----DYSNYNSGNNYAVPAGdf--gpS 232 Paenibacillus l...
YP_869253    386 Dgvsg------------------------diftPATCNGVIPVSSNNVHGQLs---yFSSYLSDKRTLSTIGEdmtlpkV 438 Shewanella sp. ...
YP_001918837 284 Dsrdlrkpmgefdd------eidfdetgplfdvYSYFPDTIAVSASDIQDNLa---sYSNYGQARVDLGAPGGdfnpedP 354 Natranaerobius ...
Feature 1                                                  #                                     
P30199       370 YGKDAWlekgymqkq----------svlstssngRYIYQSGTSLAAPKVSGALALEi----DKYQLKDQp---ETAIELF 432 Staphylococcus ...
ABA00872     370 IGYEKWvsdrlfeke----------wilvptlegKYTYAYGTSISTPKVSAALGLIi----EKYNLKDQp---DEAIRIL 432 Streptococcus u...
NP_646578    361 FGVDKWmnegymhke----------nilttanngRYIYQAGTSLATPKVSGALALIi----DKYHLEKHp---DKAIELL 423 Staphylococcus ...
Q07596       480 YGQDKFvsqgyylkd----------wlfttantgWYQYVYGNSFATPKVSGALALVv----DKYGIKNP----NQLKRFL 541 Lactococcus lac...
NP_359363    401 LGLNNFinaekyked-----------wifsatlgGYTYLYGNSFAAPKVSGAIAMIi----DKYKLKDQpynyMFVKKIL 465 Streptococcus p...
AAD56147     362 YGQEEWynqklfmke----------qvlstsnngNYDYADGTSISTGKVSGELAEIi----SNYHLQGDs---SKARSIL 424 Streptococcus m...
ZP_02613679  398 LDINDMclst--------------------aiegQYRFEVGTSIAAPKVSAIAGLLl----CKNKNLTP----KEVAEKI 449 Clostridium bot...
ZP_02326316  233 WKKERKpdfrsmcvvtypaqldqtpqskavkfdqGYEFMTGTSLAAAKMSGIIGLIqsqalEKRRQKLNk---GQIIHLL 309 Paenibacillus l...
YP_869253    439 TTTTYIdrnfidtn-------------cqgsiesCYATGQGTSYSAPIVSGLVSMVl----MQNPSLNP----DEIFNVI 497 Shewanella sp. ...
YP_001918837 355 EDPTTLclst--------------------ypgeGYAWMAGTSMASPKAAAVAGLIi----SENPGLSH----DEVITQL 406 Natranaerobius ...
Feature 1           
P30199       433 KKK 435 Staphylococcus epidermidis
ABA00872     433 YDN 435 Streptococcus uberis
NP_646578    424 YQH 426 Staphylococcus aureus subsp. aureus MW2
Q07596       542 LMN 544 Lactococcus lactis subsp. lactis
NP_359363    466 EET 468 Streptococcus pneumoniae R6
AAD56147     425 LNQ 427 Streptococcus mutans
ZP_02613679  450 YET 452 Clostridium botulinum NCTC 2916
ZP_02326316  310 RRT 312 Paenibacillus larvae subsp. larvae BRL-230010
YP_869253    498 YDS 500 Shewanella sp. ANA-3
YP_001918837 407 KET 409 Natranaerobius thermophilus JW/NM-WN-LF

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