Conserved Protein Domain Family
PX_YPT35

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cd07280: PX_YPT35 
The phosphoinositide binding Phox Homology domain of the fungal protein YPT35
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of YPT35 proteins from the fungal subkingdom Dikarya. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of YPT35 binds to phosphatidylinositol 3-phosphate (PI3P). It also serves as a protein interaction domain, binding to members of the Yip1p protein family, which localize to the ER and Golgi. YPT35 is mainly associated with endosomes and together with Yip1p proteins, may be involved in a specific function in the endocytic pathway.
Statistics
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PSSM-Id: 132813
View PSSM: cd07280
Aligned: 13 rows
Threshold Bit Score: 100.094
Threshold Setting Gi: 45185580
Created: 15-Jan-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                              ###       
Q6BIS2        42 HITDVLVGDYHVIQGEg--------------------GSSYVVWSIRIIVdds--------vysSMVIYKRYSDIQRFRE 93  Debaryomyces ha...
XP_447304     38 YIERAFVTNCTIISGEr-------------------sTPKFAVWKVTAVLhplnpn---ssgsyRIHTYKRYSDFVEFRN 95  Candida glabrat...
XP_503347    110 WAADVNIPEYNIVAGDs-------------------kAGAYVSWTIVVETky----------ggRIILRRRFSEIYEMRE 160 Yarrowia lipoly...
XP_760331    597 FAREIRIRGWSEVGSQ---------------------ARGWVVFELRIITkq----------gtPIIAHKRFSSFVKLRT 645 Ustilago maydis...
XP_001644893  34 QVQRIYVGDGSITTSD---------------------EAKFTIWKVTTELivhkvepvrvsiplEIVSYRRYSEFDQLRA 92  Vanderwaltozyma...
A6ZT13        72 SFQKAHVSDCTIVNGDh--------------------GTKFAVWRITVFLepnlkafaakresyKIQTYKRYSDFVRLRE 131 Saccharomyces c...
XP_001262040 110 WAKSVTVDDYAVVQGKt-------------------gVGAYVVWNCRIQTld----------ggPITVRMRYSEFDDLRR 160 Neosartorya fis...
XP_965321     41 WAKKVDIVDYILVNGGta------------------nIGAFVVWNIKVETln----------gsRINIRKRYSEFDSLRK 92  Neurospora cras...
EEB90237      96 FARDVNIGGWTNVGDKl--------------------GGAYVVYDCVIKTke----------gtAIHAHKRYSSFVQLYE 145 Moniliophthora ...
XP_001885128 129 FASDVQVTGWTTVAGDkppffdggqgkgkgasmvglgGGAYVVYDCVITTke----------gtSFHALKRYTAFEDLDA 198 Laccaria bicolo...
XP_720756     45 HITNVIVGEYHLISGEf--------------------GKSYMTWQIKITIndl--------dysSIVLYKRYNEIYQLRQ 96  Candida albican...
XP_001524874  42 HITDVKIGDHHLVTGQgaw---------------afrGKLYVVWQIKITIndl--------sysTVTIYKRYREILQLWA 98  Lodderomyces el...
NP_983296     30 HVAKVAVGDCTIVDARtpc---------------aplQGRLDDWTVALQLhysd-------tplSVTLRKRYSDFVRFRE 87  Ashbya gossypii...
Feature 1                               ##                     #                               
Q6BIS2        94 ALLEHYPnt--------eIPPLPPKDNFSfq----rlSMSDYWLENRRKGLQWFMTNVMLNPKHQHSp--vITHFILN 157 Debaryomyces hans...
XP_447304     96 ALLDRVRtkrpa--svseIPELPPPVKWYyswkyneiNLNKEWLANRRKGLELFINQVLLNGNIVDIakdlVIQFLRP 171 Candida glabrata ...
XP_503347    161 ALKQEFPqhk------meIPQLPAKSMFQ--------HFNKTFLQSRRRGLEYFFQCVFLNPLFASSk--vLKEFAET 222 Yarrowia lipolyti...
XP_760331    646 TLLDECKdqa------kwLPQLPTRRTGLl------sKYDAKYLEKRRRALQRWLEVVALDRVWGASe--aLRDWVLA 709 Ustilago maydis 521
XP_001644893  93 EIISKIGkn-------vqVPELPPKVYWYdywrfskiNLNRHWLNKRRQGLDNFINKIIKNKDIMEScakeVNEFLGV 163 Vanderwaltozyma p...
A6ZT13       132 NLLTRIKtakpeklnclqIPHLPPSVQWYsswkyqevNLNKDWLAKRQRGLEYFLNHIILNSSLVEMtkdiLIQFLEP 209 Saccharomyces cer...
XP_001262040 161 QLQASFPhak------naLPALPPKSVLY--------KFRPAFLESRRVGLEYFLNCILLNPEFSGSp--iVKEFLFG 222 Neosartorya fisch...
XP_965321     93 RLVQTFPnfe------aaVPLIPPKSILH--------RFQPKFLEKRRAGLQYFLNCILLNPEFSGSp--vLKEFLFS 154 Neurospora crassa...
EEB90237     146 ALQRTLPrhq-----rhyVPQLPPKAPFA--------RYRSAFYDRRRRQLEYWLSAVLLHPDVGGCk--aVRVWIMD 208 Moniliophthora pe...
XP_001885128 199 TLHKILPrrl-----rglIPPLPPKAPFA--------RFRPAFLDQRRRKLQFYLASILLNPEVGGEe--cVRRWVIR 261 Laccaria bicolor ...
XP_720756     97 DLLKIFPphdht-ittniVPSLPPKDNLSld----rlMMSKNWLEERRKGLQWFLSNVLLNPVFQNCs--vVKQFVLS 167 Candida albicans ...
XP_001524874  99 DLKEYYSedk-----eivIPQPPPKDVISvd----rvLMSNSWLEERRKSIQWFLSSVLLNPVMQKSp--vVKQFVLS 165 Lodderomyces elon...
NP_983296     88 RLLLCLPppl-----rraVPPLPPPVPWYdawrysdvNLDRAWLAARRAGLERFLQQVLLNRALVAAapvvVRGFLEP 160 Ashbya gossypii A...

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