2BJA,2BHQ


Conserved Protein Domain Family
ALDH_PutA-P5CDH-RocA

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cd07124: ALDH_PutA-P5CDH-RocA 
Click on image for an interactive view with Cn3D
Delta(1)-pyrroline-5-carboxylate dehydrogenase, RocA
Delta(1)-pyrroline-5-carboxylate dehydrogenase (EC=1.5.1.12 ), RocA: a proline catabolic enzyme of the aldehyde dehydrogenase (ALDH) protein superfamily. The proline catabolic enzymes, proline dehydrogenase and Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH), catalyze the two-step oxidation of proline to glutamate; P5CDH catalyzes the oxidation of glutamate semialdehyde, utilizing NAD+ as the electron acceptor. In some bacteria, the two enzymes are fused into the bifunctional flavoenzyme, proline utilization A (PutA). In this CD, monofunctional enzyme sequences such as seen in the Bacillus subtilis RocA P5CDH are also present. These enzymes play important roles in cellular redox control, superoxide generation, and apoptosis.
Statistics
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PSSM-Id: 143442
View PSSM: cd07124
Aligned: 26 rows
Threshold Bit Score: 692.812
Threshold Setting Gi: 116749633
Created: 30-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:2BJA: 1-Pyrroline-5-Carboxylate Dehydrogenase from Thermus with bound NADH; defined using 3.5 A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
2BJA_A          6 FRNEPIETFqte-----eaRRAMREALRRVREE---------FGRHYPLYIGGewv-dtkERMVSLNPSapsEVVGTTAK 70   Thermus therm...
YP_846320       7 FRLTYSTMFdpp----evlHARFEEALARTRAR---------LGREYGMLVNGkdl-favDKFEDRSPAdteVILGVFQK 72   Syntrophobact...
YP_001190468    3 FVNERTYQRylqenreeefHKLVDEGIRRVEEQ---------LGQEYPILIGDkel-rtpSKFNVRSPVdtsLTLGVFQR 72   Metallosphaer...
ZP_01459415     3 FRITYSMLDadl----salHAQFDQTLSSVRAR---------LGAEYPSWIAGtay-rsgDLLESRNPAnphELLGRFHR 68   Stigmatella a...
CAF18502        9 GKVTYVSVLade-----siHPAYEKALSEVWEL---------LGREWPIYIGGkev-ytsGKFEKRSPFdtsIKIGTFQR 73   Thermoproteus...
NP_968157     463 FYNEPLLDFavk-----adREKMLKALAEAKAS---------LPVNVNIVINNkel-qsgKIFDRVNPSqsdQIVGKIQM 527  Bdellovibrio ...
YP_001817604  493 FRNAANTDFtks-----aaRDQLRAALKSFADAttssrllvrRGDRRPLIIGGrei-sdrEFLPAHNPAhpsQVLGYWAR 566  Opitutus terr...
YP_844965     476 FCNEPCFDWtip-----ehRAGFRDALDRVRAT---------FPIKVPLTIGGsrf-dtpVRLRSVNPNraeEMVGDVAG 540  Syntrophobact...
NP_954435     478 FNNEAMVDFtra-----dhRAAFPKHIAQVRTQ---------LGKTYPLFINGkev-rtnDLIPTVNPNkpsEVLGQICQ 542  Geobacter sul...
EAY56884      442 FENEPFLDFsrk-----ekRLLFRAALERVRGS---------LGRQVLPRIGGreveggeETIRSVDPSlpsRTVVEVRL 507  Leptospirillu...
Feature 1                                                                                         
2BJA_A         71 agkaEAEAALEAAWKAFKTWKdwPQEDRSRLLLKAAALMRRRKRELEATLVYEVGKNWVEASADVAEAIDFIEYYARAAL 150  Thermus therm...
YP_846320      73 ggprEAHAAVAAAREAFPKWSrtPWRERAALLRKAADRIDDRIFDIGVAMALEVGKNRMEALGDAAETADLIRYSCERME 152  Syntrophobact...
YP_001190468   73 ddgeLLRSAIKLARESYRDWKdsDWRDRVELTVKAANELRRMKYELAATITFENGKNRYEAVAEVDETIDYFNYYAQLLE 152  Metallosphaer...
ZP_01459415    69 tpasEVDRVVRVAREAQRAWGatPWGERVRILRRAATLITERRMELAARMTLEVGKNRLESLGDVEEAADLLRYYAGQLE 148  Stigmatella a...
CAF18502       74 gtpeEFKAALSKAVEAFPEWSrlDWRERARILAKLADLIERDRFKLAAAITYEVGKNRFEALAEIHEAIDVLRYYVELIH 153  Thermoproteus...
NP_968157     528 atteQAEQAMQAAQTAYKTWKnvPCEQRAALVDKLADIMTRDRFKLIATQVLEVGKPWAEADGDIGEAIDFCRYYARHMR 607  Bdellovibrio ...
YP_001817604  567 atiaDADAAVAAARAAQPAWAarSANERATWAERVADLLETRRHELNALEILEAGKPWAEADADISEAVDFCRFYAAEIR 646  Opitutus terr...
YP_844965     541 agplEADAAVEAAKAAFAAWRdtPPGERAEYLFKAAAAARRIRYDLAALQVYEVGKAWSEADADVCEAIDFLEYYGREMI 620  Syntrophobact...
NP_954435     543 agttEVGDAIAAAKAAFPAWRdtDPRTRAEYLLKAAQAARKRLFELSAWQVLEIGKQWDQAYADVTEAIDFLEYYAREMI 622  Geobacter sul...
EAY56884      508 adgkMAVRALEEAKRFRETWRetSWETRIALLLRTADVLRSRRMDLAATEVFETGKSWTEADADVCEAIDFCVWYARMAL 587  Leptospirillu...
Feature 1                                           ##                         # ##               
2BJA_A        151 Ry-rYPAVEVVPypg----edNESFYVPLGAGVVIAPWNFPVAIFTGMIVGPVAVGNTVIAKPAEdaVVVGAKVFEIFHE 225  Thermus therm...
YP_846320     153 Ag-nGFVAEMGRdplqgfdsrNVSVLRPYGVWLVISPFNYPCALTGGPAGAALVAGNTVVVKPASltPWSARLIAECMRD 231  Syntrophobact...
YP_001190468  153 En-rGYTREMEGrivk--gehGISVMRPYGPWLIISPFNFPLAITATMTLGALITGNTVVVKPSSdtPLSAYKLVTTLRR 229  Metallosphaer...
ZP_01459415   149 Ea-qGFVKPMARlsp---nedTRNVLRPYGVFAVIAPFNFPVALAAGMSAGALLGGNAVILKPSEetPWCAEGLYHAFAD 224  Stigmatella a...
CAF18502      154 Km-dGYVLQMRSpip---nerPLSVLRPYGAWFVISPFNFPIALAKGMLTGVLLMGNTAVWKPTSeaPFTAALVYQLAVE 229  Thermoproteus...
NP_968157     608 ElqkPLRVGGLPge------lSHYIYKSRGVTAVIAPWNFPLAILAGMVTAAAVAGNTVVMKPAEqsTVVAWGLMKMIQE 681  Bdellovibrio ...
YP_001817604  647 RidrPRVTQPVPge------rCVERWLPRGTGVVIAPWNFPLAILCGLTIAPVVAGNTVIMKPAEqtSLVAAAFMQVLIE 720  Opitutus terr...
YP_844965     621 RlsrPKRMGHAPge------iSHLFYEPRGVAAVIAPWNFPMAISTGMTSAALVTGNTVVYKPASqsPVVGSMVMNVFEE 694  Syntrophobact...
NP_954435     623 RlgqPQRVGHAPge------lNHYFYEPKGVAAVIAPWNFPLAISMGMASAAIVTGNCVVFKPSGitSIIGWHLVELFRE 696  Geobacter sul...
EAY56884      588 Rl-kESGLSSPPge------eNRLRYEGRGIALVLPPWNFPLAIPAGMTAAALVTGNVVLLKPSLeaPLMGALLVEALLE 660  Leptospirillu...
Feature 1                       #                 #  #  #                                         
2BJA_A        226 aGFPPGVVNFLPGVGEevGAYLVEHPrIRFINFTGSLEVGLKIYEAAGrlapgqtwFKRAYVETGGKDAIIVDETADFDL 305  Thermus therm...
YP_846320     232 aGLPPGVINFVTGPGDtlGQALLDHPdVAGVTFTGSKEVGMGIWRAFArg----pwVRPVILELGGKNPTIVSRNADLDD 307  Syntrophobact...
YP_001190468  230 aGFSAEVVNYVTSPGEvvSKVMEENLdIAGFAFTGSREVGHRLLKQFIsl-----kPRPAVLELGGKNATVITAKADLRK 304  Metallosphaer...
ZP_01459415   225 aGLPPGVFQVVHGEGEslGAALVRHPaLDGVTFTGSKAVGMSIHQQLStg-----rARPCLLELGGKNPAIICRDADLEM 299  Stigmatella a...
CAF18502      230 aGVPPGVLNFVTGPGDavEEVATGDPrVAGIAFTGSRDVGMRLYRLFTqrq---pwPKPIVLEMGSKNPTVVTAKADLEK 306  Thermoproteus...
NP_968157     682 aGFPQGVINFLPGYGEevGEYIVNHKyTTTIAFTGSKAVGLHIMNRAAvvqpgqqhVKRCIIEMGGKNAVIIDNDADLDE 761  Bdellovibrio ...
YP_001817604  721 aGLPAGVVNFLPGLGEevGDALVRNPnIDFVAFTGSRAVGLQIWAAAGqtapgqanLKKVICEMGGKNALIIDNDADLDE 800  Opitutus terr...
YP_844965     695 aGLPKGVLSFLPGPGAqiGDYLVHHPdVAVIAFTGSKKVGLDIIAQANrdaeraghVKTVVAEMGGKNAIVVDADADLDE 774  Syntrophobact...
NP_954435     697 aGLPEGVFNFTPGRGSvmGDYLVDHPdISLIAFTGSMETGLRIIERAAkvhpgqanVKKIISEMGGKNAIIIDDDADLDE 776  Geobacter sul...
EAY56884      661 aGVPPGAIHYLPGRGEtiGRQLAAHPdVDLIAFTGSRAVGVSLLGLIGnpsegqkgLKHLVCEMGGKNAIVVDDDADLDL 740  Leptospirillu...
Feature 1                                                                                         
2BJA_A        306 AAEGVVVSAYGFqGQKXSAASRLILTqgaYEPVLERVLKRAERLs-VGPAEEn-PDLGPVVSAEQERKVLSYIEIGKn-e 382  Thermus therm...
YP_846320     308 AASGITRSCFGLqGQKCSACSRVLVEapvYNALADRLRAMTAKLv-VGDPTErgVTMGPVISEGAQRDFRNFARELGq-a 385  Syntrophobact...
YP_001190468  305 AVEGTFRGAFGFgGQKCSATSRVFVEssiYQEFLARFKARTETAq-IGDPRQksTFLGPLINKGAVDKFRRYVDQAIseg 383  Metallosphaer...
ZP_01459415   300 AVEGCYRSAFGLsGQKCSALSRIYVHeslQQEFITRLAEKARGVt-VGDPSLasVYMGPVINKASVQRFERAAAAARs-d 377  Stigmatella a...
CAF18502      307 AAEGVVRAAFGFgGQKCSATSRLYVQrevYDRFVERLVEKTREIvkIGDPRRreVFLGPVINKSARENYRRYVADAVaag 386  Thermoproteus...
NP_968157     762 AVDGVIYSAFGFsGQKCSAASRVIVLdevYDRFVDRLVETAKSIe-IHPAENpkAYMGPVVDKEAYDRILGTIAEAEk-n 839  Bdellovibrio ...
YP_001817604  801 AIPATLYSAFGFsGQKCSALSRLILLdevHDRFVERLLAAAAALp-VGDPAEpgTAVGPVIDAEAHGRILRMIETGRrea 879  Opitutus terr...
YP_844965     775 ALAQIVHSAFGYqGQKCSACSRLIVLeeiYDKLVERLKAAAESIh-LGPPEDpkNLMGAVIEAGARKRIMEYIELGRk-d 852  Syntrophobact...
NP_954435     777 AVPHVLYSAFGFqGQKCSACSRVIVLdavYDKFIERLVSMAKATk-VGPSEDpaNYMGAVADDKAMKSIKEYAEIGKr-e 854  Geobacter sul...
EAY56884      741 AVPAILSSAFSYqGQKCSACSRVIAVgsiHDRLAERLVRGAEALs-IGPPEGpeHRIGPVISQRALERIRGYVGRGLk-e 818  Leptospirillu...
Feature 1                                                                                         
2BJA_A        383 GQLVLGGKRLeg-----eGYFIAPTVFTEVppkARIAQEEIFGPVLSVIRVkDFAEALEVANDTPYGLTGGVYSRkREHL 457  Thermus therm...
YP_846320     386 GSFLTGGEVLtegr-yarGYFCAPTLVADVpltHRLWKTEMFVPIALVAKVdNLNEAMQIANDADYGLTAGFYGS-REEA 463  Syntrophobact...
YP_001190468  384 GRVITGGRVRed----trSYLVEPTIIVDLpytSSLWRTELFVPIVLVKEVqNLKEAIRLVNDVDYGLTAGIFSQdTEEV 459  Metallosphaer...
ZP_01459415   378 GTVHAGGERLrlegalaeGYFVAPTVVELAh-gHRLMREELFLPFVGVTGFdTLEEALVLANDGEYGLTAGIFSRkPSDV 456  Stigmatella a...
CAF18502      387 GRLRYGGRVLeege-fskGFYVEPAIIEGVapdSYLWKTELFLPILLVDKFdTLEEAVRKANDTEYGLTAGIFSEdPKEV 465  Thermoproteus...
NP_968157     840 HKLLFKGSVPg------gGFFAPPTIFGDVpgdAKLAQAEIFGPVVAVIRAkNLDQALDIANSTEYALTGGVFSRsPANI 913  Bdellovibrio ...
YP_001817604  880 TRAWQAVLPTavq--asgGHYVPPTIFTGVkpeHTLAREEIFGPVLAVLRArDLDEAFAIANSTDYALTGGLFSRsPRAL 957  Opitutus terr...
YP_844965     853 GTVLVERTVPgn-----eGFFVPLTILADLppdHRLAREEIFGPVLVVFKVkDFARAIEIANDTEYALTGGVFSRsPANI 927  Syntrophobact...
NP_954435     855 GHVLYESPVPag-----eGYFVPMTIIGGIkpeHRIAQEEIFGPVLAVMRAkDFDQAIEWANSTQFALTGGIFSRsPEHL 929  Geobacter sul...
EAY56884      819 GTLLLDREIPekir-etgGYYAGPAIFAGIrpqHVLANEEIFGPVLSILKAeSFREGISFAVDCDYRLTGGVFSRsPSHI 897  Leptospirillu...
Feature 1                                                                        
2BJA_A        458 EWARREFHVGNLYFNRKI---TGALVGVQPFGGFKLSGTNAKT-GALDYLRLFLEMKAVAERF 516  Thermus thermophilus
YP_846320     464 DRFFDGIQAGVVYANRPQgstTGAWPGFQPFGGWKGSGSSGKNaGGLYYLPLYMHEQVRTMVR 526  Syntrophobacter fumaroxidans MPOB
YP_001190468  460 EYFFKNVEAGVTYANRESgstTGAMPGVQPFGGWKDSGWTGRNaGGPYYLLSFMREQARTTYD 522  Metallosphaera sedula DSM 5348
ZP_01459415   457 EAFMDRVEAGVLYANRRTgatTGAWPGVQSFCGWKSSGSTGKGgCGPYYVAQFMREQSQTRMG 519  Stigmatella aurantiaca DW4/3-1
CAF18502      466 EYFFEHIEFGVVYANRRGgatTGAWPGAQPFTGWKASGATGRGvNGVYYLLNFAREQARTVVY 528  Thermoproteus tenax
NP_968157     914 NRVKEELEVGNLYVNRGI---TGAMVDRHPFGGFKMSGIGSKT-GGPDYLKQYMEPACVTENT 972  Bdellovibrio bacteriovorus HD100
YP_001817604  958 QRAERELVCGNVYLNRGV---TGAIVERHPFGGFKMSGSGTKA-GGRGYLENFLFPRVIAENV 1016 Opitutus terrae PB90-1
YP_844965     928 DLARREFRTGNLYINRGC---TGAVVERHPFGGFKLSGIGSKA-GGPDYLLQFMVPRNVVENT 986  Syntrophobacter fumaroxidans MPOB
NP_954435     930 AKARREFRVGNLYINRNN---TGALVERQPFGGARMSGVGTKA-GGPDYLLHFMDPRVVTENT 988  Geobacter sulfurreducens PCA
EAY56884      898 EEAKRTFRVGNLYINRGI---TGARVGRHPFGGLGFSCWGSPA-GGPDTLLQFVISRTISENV 956  Leptospirillum sp. Group II UBA

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