Conserved Protein Domain Family
PX_SNX10

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cd06898: PX_SNX10 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 10
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX10 may be involved in the regulation of endosome homeostasis. Its expression induces the formation of giant vacuoles in mammalian cells.
Statistics
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PSSM-Id: 132808
View PSSM: cd06898
Aligned: 10 rows
Threshold Bit Score: 175.597
Threshold Setting Gi: 156365508
Created: 11-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                   ###                           ##     
NP_037454     12 SVWVRDPRIqkedfwhSYIDYEICIHTNSMCFtm--kTSCVRRRYREFVWLRQRLQSNALlv-----qLPELPSKNLFFN 84  human
AAH55622      35 SVWVRDPQVhkedfwhTYISYEICLHTNSMCFrk--kTSCVRRRYSEFVWLRHKLQDNALli-----eLPKLPPWNPFFN 107 zebrafish
NP_001026157  12 TVLVRDPRTqkedswhSYIDYEIFIHTNSMCFtr--kTSCVRRRFREFVWLRQRLQSNAVli-----qLPELPSKTPFFN 84  chicken
NP_001087475  13 TIRVQDPRLqnegswtSYVDYKIFLHTNSKAFta--kTSCVRRRYREFDWLRKRLQKNSGlv-----pVPALPGKLPFLV 85  African clawed ...
XP_001626687  11 SVVVRNPRThlrgrwpQFTDYEILIWTNSTCFvs--sKSCVRRRFSEFEWLRNILEQNGSis----lqPPPLPHKRLLGR 84  starlet sea ane...
ABF22385      11 SVCVGNPRLikkdhwrSYVDYEISLHASTNSMcfrkkTSTVRRRYKEFVWLRNSLEKNALii-----eIPKLPPWNPFFS 85  torafugu
XP_001640800  47 LVEVNRPQIhic-kgsKFVDYEIYIQTNNMAFak--kLSNIRRRYSDFIWLRKQLTTNEVngfgservVPCLPPKRLFGR 123 starlet sea ane...
XP_001629456  16 DVEVKFPRThvhegkaLYTDYEVEVKTNHPCFpl--fHSNTRRRYSEFAWLRSRLGLHDFil----irTPSLPRKQLIGR 89  starlet sea ane...
XP_002222392  50 DLVIQDPQTftd-dkgRLTTYEIALETNSISFil--kSSRVRRRFEEFVWLRGQLAEIHGls-----dLPPLPSGGLLRM 121 Florida lancelet
XP_002128487  22 HVQVSNPTIhnthdegRFTTYQVTLKTNSLSFgl--kYSVVDRRYSDFTWLRRALARTCDv------kPPSLPFPGIIGT 93  Ciona intestinalis
Feature 1                    #                              
NP_037454     85 mn---nrQHVDQRRQGLEDFLRKVLQNaLLLSDSSLHLFLQSH 124 human
AAH55622     108 ln---neFHITQRMQGLQQFLEAVLQTpLLLSDSRLHLFLQSQ 147 zebrafish
NP_001026157  85 mn---npHHVDHRRQGLQEFLEKILQNaLLLSDSRLHLFLQTQ 124 chicken
NP_001087475  86 gn---neDFIERRRQGLQQFLEQIVQNmVLLSDSRLHLFVQSQ 125 African clawed frog
XP_001626687  85 fn----qSFIKERQQELQDFLVRVLAVtSYLSEAALHLFLQTN 123 starlet sea anemone
ABF22385      86 lr---nmGCVLPRMEGMQEFLESVLHTpLLLSDSRLHLFLQSD 125 torafugu
XP_001640800 124 fe----pKFVFSRKHGLQDFLDKVLSIrDFLSYSGLHLFLQSK 162 starlet sea anemone
XP_001629456  90 fk----eGFLCERQAGLQKFMNRVVETsIYLQEPVLQLFLQTN 128 starlet sea anemone
XP_002222392 122 fgrqfdkEFIRERQKLLQQFTEKIVREpEILSDPMLHLFLQTQ 164 Florida lancelet
XP_002128487  94 fr----kRYLEERQQILTDFLQKVVRQtVFLSNTALHLFLQTR 132 Ciona intestinalis

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