1XTN,1XTE,1XTN


Conserved Protein Domain Family
PX_CISK

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cd06870: PX_CISK 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology Domain of Cytokine-Independent Survival Kinase
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Cytokine-independent survival kinase (CISK), also called Serum- and Glucocorticoid-induced Kinase 3 (SGK3), plays a role in cell growth and survival. It is expressed in most tissues and is most abundant in the embryo and adult heart and spleen. It was originally discovered in a screen for antiapoptotic genes. It phosphorylates and inhibits the proapoptotic proteins, Bad and FKHRL1. CISK/SGK3 also regulates many transporters, ion channels, and receptors. It plays a critical role in hair follicle morphogenesis and hair cycling. N-terminal to a catalytic kinase domain, CISK contains a PX domain which binds highly phosphorylated PIs, directs membrane localization, and regulates the enzyme's activity.
Statistics
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PSSM-Id: 132780
View PSSM: cd06870
Aligned: 9 rows
Threshold Bit Score: 182.222
Threshold Setting Gi: 167521840
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..dimer interface
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:The PX domain of CISK targets highly phosphorylated phosphoinositides such as phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) and PI(3,4,5)P3.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                           ###                      ##  
1XTN_A         6 SCPSVSIPSSDEHREK-------------KKRFTVYKVLVSVGrSEWFVFRRYAEFDKLYNSLKKQFPamALKIPAKRIF 72  house mouse
1XTE_A         6 SCPSVSIPSSDEHREK-------------KKRFTVYKVLVSVGrSEWFVFRRYAEFDKLYNSLKKQFPamALKIPAKRIF 72  house mouse
1XTN_B         6 SCPSVSIPSSDEHREK-------------KKRFTVYKVLVSVGrSEWFVFRRYAEFDKLYNSLKKQFPamALKIPAKRIF 72  house mouse
NP_001026111   6 SCPSVSIPSSDEHREK-------------KKRFTVYKVLVSVGrNEWFVFRRYAEFDKLYNTLKKQFPtmNLKIPAKRIF 72  chicken
NP_001103937   6 SCPNVSIPCYNEQRDK-------------KKRYTVYKVMVSVGrHEWFVFRRYAEFDKLYNTLKKQFPalNLKIPAKRIF 72  zebrafish
XP_001745258   3 SEVEDATIVAHETRRD-------------DKRFTVYKIELVLGsRKVAVFHRYSEFRELYEQLKEAFPreKFKFPSKRFI 69  Monosiga brevic...
XP_001946554  13 TCENCSKLSMDEDDDKtaamvsqaeiwqeDKKFTVYKIVIWSRnESWHVFRRYNEFYKLSEVLKKQVPdsNLKIPGKKLF 92  pea aphid
EEC06314      21 PPLTTASVTNSEVHEK-------------NKKFTVYKVVVTVDgHSWFVLRRYNDFSKLLDIIKKQCPgsHLKLPGKKLF 87  black-legged tick
EEB10981       9 VQTARVQDSETWDIEK-------------RSKYTVYKVVVHSGsSSWFVFRRYAEFHKLFESLRKQFPnlQLKLPGKKLF 75  human body louse
Feature 1                   #                              
1XTN_A        73 GdNFDPDFIKQRRAGLNEFIQNLVRYpELYNHPDVRAFLQMD 114 house mouse
1XTE_A        73 GdNFDPDFIKQRRAGLNEFIQNLVRYpELYNHPDVRAFLQMD 114 house mouse
1XTN_B        73 GdNFDPDFIKQRRAGLNEFIQNLVRYpELYNHPDVRAFLQMD 114 house mouse
NP_001026111  73 GdNFDPDFIKQRRAGLNEFIQNLVRQpELCNHPDVRAFLQMD 114 chicken
NP_001103937  73 GdNFDPEFIKQRRAGLHEFIQRIVSHsQLCSHPDVKVFLQMD 114 zebrafish
XP_001745258  70 G-NFDPDFIKTRKRGLHEFIQRVVSDpKIVTHPAVRDFMMAT 110 Monosiga brevicollis MX1
XP_001946554  93 CnNMDPSFIASRREGLDQFVHHLMHQnNLLNISEVRIFFNLD 134 pea aphid
EEC06314      88 GnNFSPDFIRTRRQGLDDFIQKLVLDeKLMQHPEVRAFLNVD 129 black-legged tick
EEB10981      76 GnNLDPNFVAMRQDGLDNFVQQIISHtILLQLNKFRLVLTYN 117 human body louse

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