Conserved Protein Domain Family
PX_SNX8_Mvp1p_like

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cd06866: PX_SNX8_Mvp1p_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX8 and the yeast counterpart Mvp1p are involved in sorting and delivery of late-Golgi proteins, such as carboxypeptidase Y, to vacuoles.
Statistics
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PSSM-Id: 132776
View PSSM: cd06866
Aligned: 22 rows
Threshold Bit Score: 151.612
Threshold Setting Gi: 19115109
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                              ###                       #
NP_037453      75 DTVQVELIPEKKGLFL-KHVEYEVSSQr------------------fKSSVYRRYNDFVVFQEMLLHKFPYRMVPALPPK 135  human
Q7SB97        412 ENILVTLMPEKEGVFMfQHHNYEVSSIr------------------rGSKVVRRYSDFVWLLDCLHKRYPFRVLPLLPPK 473  Neurospora cr...
XP_001747511 1149 SEFVQVMLGHATGMLK-KHTNYEVFSKr------------------kSTTVSRRFSDFEPLDAYLRKRYPYRIIPQLPPK 1209 Monosiga brev...
XP_499725     227 DSVTLAIVPEREGMFLfRHVNYSISSVsg----------------tdRITVIRRYSDFSWLQDYLLKKYCFRQVPLLPPK 290  Yarrowia lipo...
EDV11508      130 DIIIIEEIPEREGLLF-KHANYLVKHLialps----------tspseERTVVRRYSDFLWLREILLKRYPFRMIPELPPK 198  Saccharomyces...
XP_756637     304 DQVTVRLRSELEGFII-KHNVYIVSSSl------------------rKSQVTRRYSDWLWLAECLVKRYPFRCLPVLPPK 364  Ustilago mayd...
XP_001875107  241 ETVKVTILGHQGFILN-RYLVYEVTTEr-------------------GGSVPRRYSEFVFLWECLTRRYPFRLFPALPPK 300  Laccaria bico...
NP_983390     143 NIVVVEEIPEREGILF-KHTNYLVKHLvvlpn----------tnpssNQTVIRRYSDFNWLQEVLLKKYPFRMIPELPPK 211  Ashbya gossyp...
XP_571433     211 FRSTDPMVDNAEDRWW-KDQERIVVTLipekegwflqkyriesdkrgEGPVARRYSDFVWLMDVLEKRYPFRILPPLPPK 289  Cryptococcus ...
NP_594197     285 LEMNVDIDLEPSGYFFyRHNNYIISDSs------------------nTREVLRRYSDFFWLHSYLMKKYPFRRVPLIPLK 346  fission yeast
Feature 1         #                   #                              
NP_037453     136 RMlg--------aDREFIEARRRALKRFVNLVARHPLFSEDVVLKLFLSFS 178  human
Q7SB97        474 RVafngn-hlsndGASFIEKRRRGLARFLNALVRHPVLGQEQLVIMFLTVP 523  Neurospora crassa
XP_001747511 1210 TFggg------nmKPEFIEHRRRGLQRYLMFVGRHPVLQDDEILTAFLTDS 1254 Monosiga brevicollis MX1
XP_499725     291 RLavngh--ylssDNYFLERRRRGLTRFINQVLRHPVLGQDEAVRTFVTLR 339  Yarrowia lipolytica CLIB122
EDV11508      199 RIgsq------naDQLFLKKRRIGLSRFINLVMKHPKLSNDDLVLTFLTVR 243  Saccharomyces cerevisiae RM11-1a
XP_756637     365 RIampiagrhlsaDDLFIERRRRGLERFLRMLTCHPMLREDKLVEVFFTEP 415  Ustilago maydis 521
XP_001875107  301 RIg---------aDEQFLEQRKRGLSRALSFVINHPVIKEDGLLAVFLTEP 342  Laccaria bicolor S238N-H82
NP_983390     212 KIgaq------naDPIFLARRRKGLCRFINLVIMHPVLKQDDLVLTFLTVP 256  Ashbya gossypii ATCC 10895
XP_571433     290 RIn---------pSSAFLEARRLALIRLLSFLTAHPVLRTDACLNIFLTSS 331  Cryptococcus neoformans var. neoformans JEC21
NP_594197     347 KFhsk-----cfnSKQFFRTPPPGLSDFVNDLSHHPIFSNDEVVRVFFTEP 392  fission yeast

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