2W35,3GOC,3GA2


Conserved Protein Domain Family
Endonuclease_V

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cd06559: Endonuclease_V 
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Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases
Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.
Statistics
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PSSM-Id: 143472
View PSSM: cd06559
Aligned: 121 rows
Threshold Bit Score: 168.062
Threshold Setting Gi: 56404421
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Active_site
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:Active_site [active site]
Evidence:
  • Comment:The active site includes a base lesion-recognition pocket, a strand-separating wedge and a catalytic pocket.
  • Structure:2W35, Thermotoga maritime, the strand-separating wedge of EndoV positioned to present deaminated bases to the hydrophobic lesion-specific pocket at the active site using Mg2+ as a cofactor
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                            # ##                                        
2W35_A        13 PEEAIKVQNELRKKIKltpy------egepEYVAGVDLSFpg--------kEEGLAVIVVLeypsFKILEVVSERGEITF 78  Thermotoga mari...
ZP_01691647    5 ETQWANEQRLMAAQVQiptlgqg-yqpqdnDVILGLDIQYe---------sDKAYIAGDLQhy-nGHHLGTYSGVTAVMA 73  Microscilla mar...
YP_001940261  10 IEQLILEQKRMASLVFppweg-----slgsIRCGGVFICYergkreagkrgDRGWAAFVLIf--pDGKTQSFCVEGRTPF 82  Methylokorus in...
YP_002428557   5 YQRAVMLQRILSERVLaeldsfpridpsriRSVAGVDASYr---------gGVQVGSAVLMdyraKMPLAYTCLTSKPPI 75  Desulfurococcus...
YP_001040938   7 YRRAVRIQRALAEKAFksienigridldkiKYVVGVDAAYh---------kGLMYGVAVLLdfssGNVLEHSVVIKKPPI 77  Staphylothermus...
YP_001434927   5 RRRASELQRKLSELVKeedcf----dpeavEAVGGLDVSYkg--------dVGVSALSLIDyk-tLRPLKHYYVVARVPI 71  Ignicoccus hosp...
YP_002246849   4 KERFQRAQLALRSIKEdfsl------nqcvHTVAAFDVSFq---------aGLGVGVVALFnyqsVQLMECEIVVKEPYV 68  Coprothermobact...
O58394         2 LERIANIQKKLSKSIVerki-------nevRKVAAVDVSYk---------eEKARAALVITtfpeGEVLKTKVIETTVSF 65  Pyrococcus hori...
YP_183319      6 FKKLEEVQRKLAERIVerple-----vskiKTVGAVDVSYr---------dERARAAFVLCsfpdCELLKQRVVEVDVSF 71  Thermococcus ko...
YP_001736655 103 FKVMASDQVKLSEKLIlsel-------gdvSVAAGVDVSYi---------dDRAVACCVTVde-eLKIIEVRFERFSPQI 165 Candidatus Kora...
Feature 1         # ####                             ###   #    ##               #####           
2W35_A        79 PYIPGLLAFREGPLFLKAWEKLrt-----kpDVVVFDGQGLAHPRKLGIASHMGLFIEIPTIGVAKSRLygtfkmpedkr 153 Thermotoga mari...
ZP_01691647   74 PYVPGFFCFREGPPLFALVNHLlndatlprpDLLVVDGHGIAHPRTFGVACWLGVKTGLPTIGIAKKSLlkfsgqvgneq 153 Microscilla mar...
YP_001940261  83 DYVPGCLALREGPLLEEALLGLgs-----lpDLLFVNATARDHPYRAGLALHLGFKLDIPSIGITRKPLlaegtlpgnkr 157 Methylokorus in...
YP_002428557  76 PYVPGLLAFREAPVYIKALHRLpa-----kpDIILVDGHGLSHPRAFGIATHIGLVLSTPSIGVAKKPLygeveevng-- 148 Desulfurococcus...
YP_001040938  78 PYVPGLLAFREAPAYISAVLKLrh-----epDIIFVDGHGLSHPRGLGIATHIGLVLDKPSIGVAKKKLygeireing-- 150 Staphylothermus...
YP_001434927  72 PYVPGFLAFREAPLHLTLIKKVkg------yDLLLVDGHGRTHPRGLGIASHVGVTSGVPTVGVAKRRLvgeeercge-- 143 Ignicoccus hosp...
YP_002246849  69 PYVPTYLAFREMPYILALYKKLhe-----epDLLLIDGHGKSHPRGMGIATQAGLSLRKPSIGVAKRLLfgdlletadg- 142 Coprothermobact...
O58394        66 PYIPTFFFLRETKPILIATKGEt-------fDVLIVEGHGKAHPRGYGLASHIGVVLRKPTIGVAKRLLkntpkdty--- 135 Pyrococcus hori...
YP_183319     72 PYIPTFFFLRETRPVLIALGKEr-------pDVLLVEGHGRAHPRGYGLASHIGLVLGIPTIGISKRLLrg--------- 135 Thermococcus ko...
YP_001736655 166 PYVPTYLAFRELRGMLPVSMKCd-------fDAIFVDGHGVLHPRGLGEASHLGLLLGKPSIGVAKSKLvgevegg---- 234 Candidatus Kora...
Feature 1                                                                         #   # 
2W35_A       154 cswsylydgeEIIGCVIRtkegsapIFVSPGHLm-DVESSKRLIKAFTLp----gRRIPEPTRLAHIYTQR 219 Thermotoga maritima
ZP_01691647  154 gsildiahkgQVVGYALRtqtgikpEYVSPGHLv-ALDTAKTVALNLVSe-----YRIPDVLRRADQMARL 218 Microscilla marina ATCC ...
YP_001940261 158 getselrignQAVGFWLRtqdnkapLVVHPGYRi-DFEKAKEIALFYTEr-----YRTPQPLRLARQLSRL 222 Methylokorus infernorum V4
YP_002428557 149 --rklvrahgRIVGEVVEtnq-gseIYVSIGYLi-RLEDAVEVVRHLMEp----gLKLPLPIHLADNYSRS 211 Desulfurococcus kamchatk...
YP_001040938 151 --ekyleahgMIVARIIKhkg--qeLYISIGYKi-TLEDAYLLTTKLLTp----kYKLPLPTAIADQLSKR 212 Staphylothermus marinus F1
YP_001434927 144 --reclvhegKVVAYVIRrgk--qkLYVSVGHCv-SLETAYQIVKRLTV------RRLPEPIAWADRISRS 203 Ignicoccus hospitalis KI...
YP_002246849 143 sslvvhpetrEPLAVAYKekprfkpVFISVGAGvsNLSEAVSLVLPLFKg-----HREPEPLRYVHNVSLQ 208 Coprothermobacter proteo...
O58394       136 ---kkvgkvyVSVGNLITled-atkIIRAILDEs-GYPKPLKLADKLSKgriyevKNTPSPNRSRKKRGNR 201 Pyrococcus horikoshii
YP_183319    136 ---------tPEGSWVKVg-----kAYVSVGHLi-DLPSAVEVVKTLNK------NGYPLPLRIADRLSRG 185 Thermococcus kodakarensi...
YP_001736655 235 ----yvkfmgRNSAALHRg------GFISPGHLs-DLESALKASLKFWKe-----GNQPFPLKLAHSLSKS 289 Candidatus Korarchaeum c...

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