1GAQ,2OK7,1JB9,2B5O


Conserved Protein Domain Family
CYPOR_like_FNR

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cd06208: CYPOR_like_FNR 
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These ferredoxin reductases are related to the NADPH cytochrome p450 reductases (CYPOR), but lack the FAD-binding region connecting sub-domain. Ferredoxin-NADP+ reductase (FNR) is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins, such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap between the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2, which then transfers two electrons and a proton to NADP+ to form NADPH. CYPOR serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases, sulfite reducatase, and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. CYPOR has a C-terminal FNR-like FAD and NAD binding module, an FMN-binding domain, and an additional connecting domain (inserted within the FAD binding region) that orients the FNR and FMN -binding domains. The C-terminal domain contains most of the NADP(H) binding residues, and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule, which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99804
View PSSM: cd06208
Aligned: 16 rows
Threshold Bit Score: 359.713
Threshold Setting Gi: 134105219
Created: 22-May-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1GAQ: Zea mays FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Structure:2OK7: Plasmodium falciparum FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Structure:2B5O: Synechococcus ssp. FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1GAQ_A       30 NLYKPKEPYVGRCLLNTKITGdda--------pgETWHMVFSt-eGKIPYREGQSIGVIADGvdk--------------- 85  maize
2B5O_A      115 NIYRPKTPFLGKCIENYELVDegg--------sgTVRHVTFDiseGDLRYLEGQSIGIIPPGedk--------------- 171 Synechococcus sp...
AAW79314     83 NIFMPKAPYTGKVVANDVHPQtltedt--gdanwETTHLTFDh-dGKVPYLEGQSIGIIAPGpdk--------------- 144 Heterocapsa triq...
NP_925241    14 NIYRPASPFRGKALQNINLTPddp--------dnDVRHVVLDlsgGDLRYFEGQSIGIVPPGtda--------------- 70  Gloeobacter viol...
BAF42337     58 NLFKPSSPLVGTCIYNKKIVGdda--------pgDTCHVIIHh-dGKLPYLEGQSVGIIPEGtdd--------------- 113 Cyanidium caldarium
ABK22336     98 NKYKPKTPYIGRVLLNTKITAdda--------pgETWHMVFSt-eGELPYREGQSIGVIPTGidk--------------- 153 Sitka spruce
CAC15394    163 NTFRPASPLICRVVSVTPVTSkdsspddshgeapQVFSIVLHh-gKQLPFVEGQGIGIMPPSraaqapvadestsqrnsv 241 Toxoplasma gondii
YP_841256   115 HLYSRAAPAVATVTLRERATAlda--------ddDVWHIVLDfgnQAMPILEGQSIGILPPGlda--------------- 171 Ralstonia eutrop...
AAN32622    140 NLYTPTRPLTATVSGNYQATAdda--------ssDIHHIVLDfgaTPFPVLEGQSIGIIPPGtda--------------- 196 Thauera aromatica
ZP_01012910 130 NLYNLGKPIEATVQGNYRLTAeds--------daDVRHIILDfagQPMPVLEGQSIGIIPPGeta--------------- 186 Rhodobacterales ...
Feature 1                             ####                 # # # #                             #
1GAQ_A       86 ---------------ngKPHKVRLYSIASSAIGdfgdskTVSLCVKRLIYtndag--------------------eivkG 130 maize
2B5O_A      172 ---------------ngKPHKLRLYSIASTRHGdmednkTVSLCVRQLEYqdpes-------------------getvyG 217 Synechococcus sp...
AAW79314    145 --------------kgeTPAKIRLYSIASSAVGddqtskTVSLCVKRVVEldgkfanrevgedkpdkagtgypknkvyrG 210 Heterocapsa triq...
NP_925241    71 ---------------qgKPHKLRLYSIASSRIGdnkdgkTVSLCVKRVVYkhpet-------------------geivrG 116 Gloeobacter viol...
BAF42337    114 ---------------kgRPHKLRLYSIASTAAGdfgdykTLSLVVKRLVYtnekg--------------------eevrG 158 Cyanidium caldarium
ABK22336    154 ---------------ngKPHKLRLYSIASSALGdfgdskTVSLCVKRLVYtndqg--------------------evvkG 198 Sitka spruce
CAC15394    242 qpdgqgqqpttvasickRRLLPRIYSIASSRDGddgcgsTLTLCVKKHIYadpvtgk----------------rdrqkdG 305 Toxoplasma gondii
YP_841256   172 ---------------ngRPHIERLYSVASARTGerrgtsTVALTVRRKPG-----------------------------G 207 Ralstonia eutrop...
AAN32622    197 ---------------ngKPHLLRMYSVASPREGerphhnNLSLTVKRVTEdhdg---------------------hparG 240 Thauera aromatica
ZP_01012910 187 ---------------dgKPHLPRLYSVSSPRDGerpgynNLSLTVKREEQ-----------------------------G 222 Rhodobacterales ...
Feature 1       ###                                       #                                     
1GAQ_A      131 VCSNFLCDL-QPGDNVQITGPVGKEMLMPkdpnATIIMLATGTGIAPFRSFLWKMffek-------hddyKFNGLGWLFL 202 maize
2B5O_A      218 VCSTYLCNLpVGTDDVKITGPVGKEMLLPddedATVVMLATGTGIAPFRAFLWRMfkeq-------hedyKFKGKAWLIF 290 Synechococcus sp...
AAW79314    211 VCSNHICDM-SVGDDVLITGPTGAEMLLPedpkANIIMLATGTGIAPMRSYMRLLfhdkage--aadgsrKFQGLAWLFM 287 Heterocapsa triq...
NP_925241   117 VASNFICDL-APGDDVSITGPTGKTFLLPedpsTNLVLIATGTGIAPFRAFLRRIyde---------mdtPWQGKVWLFF 186 Gloeobacter viol...
BAF42337    159 VCSNFLNDI-KPGEPIKMTGPVGKEMLMPddpnATIIMLATGTGIAPFRAFMRKAfvek-------hadyQFKGKMILYL 230 Cyanidium caldarium
ABK22336    199 VCSNFLCDL-KPGEEVTITGPVGKEMLMPvdpnATIIMLATGTGIAPFRGYLWKMffek-------hpdyKFNGLAWLFL 270 Sitka spruce
CAC15394    306 ICSTYICDA-KCGDEVEVTGPVGKALLLPtsteTPLVMLATGTGVAPFRSHLQALrrkllsaggpsaaqpANRPKVLLFI 384 Toxoplasma gondii
YP_841256   208 LCSNYLCGL-VPGETVRVVGPLGDTFLMPmeasANLIMVCTGTGVAPFRGFIQHRlrs----------mrGATGSLMLFF 276 Ralstonia eutrop...
AAN32622    241 IASNYVCDL-KKGDKVQVTGPYGSTFLMPnhpgSSLLMICTGTGSAPMRAMTERRrrr---------meqKEGGELMLFF 310 Thauera aromatica
ZP_01012910 223 ICSNYVCDL-KKGDKVRVTGPFGSTFLLPsdpnAELLMICTGTGSAPFRGFTMRRqre----------mpTATEKMTLVF 291 Rhodobacterales ...
Feature 1                                                                                       
1GAQ_A      203 GVPTsSSLLYKEEFGKmker-apeNFRVDYAVSREQtnaagERMYIQTRMAeykEELWELLKKDntYVYMCGLKGMEKGI 281 maize
2B5O_A      291 GVPYtANILYKDDFEKmaae-npdNFRLTYAISREQktadgGKVYVQSRVSeyaDELFEMIQKPntHVYMCGLKGMQPPI 369 Synechococcus sp...
AAW79314    288 GVPYsKSLLYDDEHLAyvkn-ypdQFRFDYAVSREQtnaagQKMYIQTKMAeytDELWELMQKEntHIYMCGLKGMEAGM 366 Heterocapsa triq...
NP_925241   187 GMQNsNSYLYQDELAGyta---kgDFEIVEAISREQknaqgGRMYVQHRIAehaAALWELISGGntYTYICGLKGMEDGI 263 Gloeobacter viol...
BAF42337    231 GVPTsSSLLYRDELEEmkan-fpdQVELHYAISREMknkqgGRYYLQDSMAergEEIWQLLRKDntYVYMCGLKGMDSGI 309 Cyanidium caldarium
ABK22336    271 GVPTsSSLIYKEEFEKmkek-spdKIRVDFAVSREQtnekgEKMYIQTRMGeyaKELWGLFKKEntYVYMCGLKGMEKGI 349 Sitka spruce
CAC15394    385 GARTaAAVPYMNEWRDieaqrdgnFVDIHFALSRQMknpqgKKLYIQDVVWqerEKVWKALDRDggHLYACGLKNMMVGV 464 Toxoplasma gondii
YP_841256   277 GGRRpVELPYCGEEDGlp----egFVDHYFCFSRQPd---aPRIYVQDGIRaagKRIRTLLQDEltHVFLCGRKGMEAGV 349 Ralstonia eutrop...
AAN32622    311 GARApGELPYFGPLKKlp----edFIDINFAFSRVPd---ePKRYVQDRIReraDKVFRMLTDDnaFIYICGLKGMEAGV 383 Thauera aromatica
ZP_01012910 292 GARKpGELPYFGPLKKvp----esFLRKVFAFSRLDn---ePKHYVQDKLReekEAVAKLLKSDnaYFYICGKKEMEHGV 364 Rhodobacterales ...
Feature 1                                            #
1GAQ_A      282 DDIMvslaek-dgidwFDYKKQLKrg----dQWNVEVY 314 maize
2B5O_A      370 DETFtaeaek-rglnwEEMRRSMKke----hRWHVEVY 402 Synechococcus sp. PCC 7002
AAW79314    367 EECFsakaea-aglvwKEFAKSMKka----dRYHVEVY 399 Heterocapsa triquetra
NP_925241   264 DDAFtaaa-----aakDTVWKDYRynlkqsgFWHVETY 296 Gloeobacter violaceus PCC 7421
BAF42337    310 DSFMtdla-----akdGVNWADFKkqlkqqhRYNVEVY 342 Cyanidium caldarium
ABK22336    350 DDIItsla-----aeeGIDWNEYKrqmkkseRWNVEVY 382 Sitka spruce
CAC15394    465 HEVLgnmae--ekglpRDHLASLLkh---qrRWHVEVY 497 Toxoplasma gondii
YP_841256   350 EDAFagvlrgdgasawPMVRERLLas----gRYHVETY 383 Ralstonia eutropha H16
AAN32622    384 LEAFrdic-----rahGTDWEALRpellakgRFHVETY 416 Thauera aromatica
ZP_01012910 365 EEALadiargeglewkPIRDKMREd-----gRYHVETY 397 Rhodobacterales bacterium HTCC2654

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