Conserved Protein Domain Family
M20_Acy1L2_AbgB

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cd05673: M20_Acy1L2_AbgB 
M20 Peptidase Aminoacylase 1-like protein 2 aminobenzoyl-glutamate utilization protein B subfamily
Peptidase M20 family, ACY1L2 aminobenzoyl-glutamate utilization protein B (AbgB) subfamily. This group contains mostly bacterial amidohydrolases, including gene products of abgB that catalyze the cleavage of p-aminobenzoyl-glutamate, a folate catabolite in Escherichia coli, to p-aminobenzoate and glutamate. p-Aminobenzoyl-glutamate is a natural end product of folate catabolism, and its utilization is initiated by the abg region gene product, AbgT, by enabling uptake of its into the cell in a concentration-dependent, saturable manner. It is subsequently cleaved by AbgA and AbgB (sometimes referred to as AbgAB).
Statistics
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PSSM-Id: 349922
Aligned: 14 rows
Threshold Bit Score: 581.953
Created: 23-Apr-2007
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
metal binding
Feature 1: metal binding site [ion binding site], 5 residue positions
Conserved feature residue pattern:[DEC] H [KE] H HClick to see conserved feature residue pattern help
Evidence:
  • Comment:defined by homology to related sub-groups
  • Comment:conserved metal-coordinating cysteine in this subfamily differs from archetypal M20 motif

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
CAG76983   42 IAAKRQQFCTLSDGIWDTPELNYEEhRSAAQHEAILKaeGFRLTKGIANmPTALLGEFG--EGLPIVAILGEYDALPGLS 119 Erwinia carotovora...
NP_420367 103 VESRAKLAQVMNDKIFSFGELGFQEvETSAYITKVLEenGFTIQRGVSGiPTAWTATWThgQGGPTIALGSDIDCIPKAS 182 Caulobacter cresce...
AAK90015   13 IEAMKPDFTTLSDSIWDFAELKFEErQSARVLANALEenGFVVRRGVAGmDTAFIGEFG--QGNPIIAFLGEFDALAGMS 90  Agrobacterium tume...
AAC74419   12 IEADRQRYTDIADQIWDHPETRFEEfWSAEHLASALEsaGFTVTRNVGNiPNAFIASFG--QGKPVIALLGEYDALAGLS 89  Escherichia coli s...
EDM98452   11 VEENSAVFEDVSDQIWGFAEMCYQEkQSAELQKKVLRelGFTVNDQIKGmETAFIASYG--EGHPIIAILGEYDALAGLN 88  Bacteroides capill...
EDM89115   35 IEEKKSLIVGIADKVWEFAELSLQEfKSAETYCEALEkeGFDVERGTCNiETAFAASYG--HGRPYIGILAEYDALSGLS 112 Ruminococcus obeum...
CAJ67127   13 IDEIQGELRDISDKIWKNPELQYKEyYASNLQKECLKkhGFSIEGLEDM-DTAFVASYG--EGRPVIAVLGEYDALDGLS 89  Clostridium diffic...
CAJ67683    9 VEEIREELIDLSKKIWENPELAFEEkYASSIQKEYLKskGFKIEEVENL-PTGFIASFG--EGKPVIGILGEYDALPELS 85  Clostridium diffic...
CAJ70005   10 IDKKRFKLDDLSKKIWENPEKAFKEfKACENTANFLRseGFDVEVGVGGlATAIRASFG--SGKPVIGFMGEFDALPGLN 87  Clostridium diffic...
AAR37815   17 IESRAQQLTDLSDHIWDLAEVGYQEtLSSNALKNALLaeGFSIESGVASiPTAFVASYG--SGQPVIAIMGEFDALPGIS 94  uncultured marine ...
Feature 1                         # #                                   #                     
CAG76983  120 QQAnvaePKPLENGg-NGHGCGHNLLGTAALQAATAVKDYLQKHSLAGTVRFYGCPAEEGGSSKGFMVKEGVFDDVDIAI 198 Erwinia carotovora...
NP_420367 183 QKPgvpwHDPIIPGa-PGHGEGHNSGQAVNVVAALAVKDLMVKQNIAGTLVLWPGVAEELVAGKAYMVRDGVFKGVDATI 261 Caulobacter cresce...
AAK90015   91 QVAgvaeAKPLEPDa-TGHGCGHNLLGVGSLMGAIALARHLKGNNLPGTVRYYGCPGEEGGSGKTFMVRAGLFDDADAAL 169 Agrobacterium tume...
AAC74419   90 QQAgcaqPTSVTPGe-NGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAAL 168 Escherichia coli s...
EDM98452   89 QEAdcteHKPMEPGk-PGHGCGHNLLGAGSMCAVNAVKKYMEAHNVKGTIRYYGCPAEEGGSAKTFMVRDGYFKDCDIEL 167 Bacteroides capill...
EDM89115  113 QEGglieRKEKNAGg-NGHGCGHNLLGAGAFAAALGIKAYLEQNDVSGTVILYGCPGEEGGAAKAFMARDGLWKKLDAAL 191 Ruminococcus obeum...
CAJ67127   90 QKVst-tKEPIVDGg-AGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFFDGIDCAF 167 Clostridium diffic...
CAJ67683   86 QCVsa-eRKPLVEGk-AGHGCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVDCAF 163 Clostridium diffic...
CAJ70005   88 QKVst-kQEAFELGa-YGHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEGLDLAI 165 Clostridium diffic...
AAR37815   95 QAAv--pVVQKIDGkkTAHACGHHLFGAGSVGAAIAVKEWLDRTETSGTVQFFGTPAEEGGSGKVYMVRAGLFDDVDTVI 172 uncultured marine ...
Feature 1       #                                                                             
CAG76983  199 CWHPatf--tgVNSPVSLACNELNFYFKGRAAHAASSPHLGRSALDAVELMNVGVNYMREHMPSSARVHYAITDSGGhAP 276 Erwinia carotovora...
NP_420367 262 FTHVganlqttWGQPSGTGLVSVKYSFHGESAHSAGAPWRGRSALDAVELMNIGWNMRREHLRPEQRSHHVISDGGD-QP 340 Caulobacter cresce...
AAK90015  170 TWHPapf--ngVRSTNNLAVLEYFYRFKGVAAHASNAAHLGRSALDAVELMNVGVNFLREHMPQDCRVHYAITDTGGkAA 247 Agrobacterium tume...
AAC74419  169 TWHPeaf--agMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGiSP 246 Escherichia coli s...
EDM98452  168 SWHPaqf-nygWDGEDCLGTYNILFKFKGISAHAAACPQLGRSALDACELMNVGVNYLREHVIPEARIHYAYLNAGGtAP 246 Bacteroides capill...
EDM89115  192 TWHPedv--neVATGSSNSCIQTQYKFTGIASHAAGAPEKGRSALDAVELMNIGVQFLREHMSDKARIHYAITDAGGcSP 269 Ruminococcus obeum...
CAJ67127  168 SWHPfdi--ntPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNIGCNYLREHIFDSCRVHYVTTNGGK-MP 244 Clostridium diffic...
CAJ67683  164 TWHPfdi--naPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANYLREHVIDSIRMHYVITNGGG-RP 240 Clostridium diffic...
CAJ70005  166 NFHPnki--seATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGANYLREHVTSDVRIHYTITDGGV-AP 242 Clostridium diffic...
AAR37815  173 HWHPasv--nsADSASTLANRSAKFTFTGVSSHAASAPERGRSALDAVESMNFMVNLLREHVPSSTRLHYVITNGGA-TP 249 uncultured marine ...
Feature 1                                                                                     
CAG76983  277 NVVQANATVRYLVRARQlPELHQLVKRVKKIAEGAALMTETEVSSEVIsGDANLLANPPLEARMHEHLLALGpIAFDDED 356 Erwinia carotovora...
NP_420367 341 NVVPSEATVWYYFREQTfDAIKKSWAIGDKIAKAAADMTDTKVSSAIVgTAAPRHFNRPMAEAAQKNIEQVGlPKWTEDE 420 Caulobacter cresce...
AAK90015  248 NVVQAKAEVLYLIRAPEiRQALDLAARVDKVARGAAMMTETEVEIVFDtASTNLLPNITLETAMHENMVTLGpVAFDEAD 327 Agrobacterium tume...
AAC74419  247 NVVQAQAEVLYLIRAPEmTDVQHIYDRVAKIAEGAALMTETTVECRFDkACSSYLPNRTLENAMYQALSHFGtPEWNSEE 326 Escherichia coli s...
EDM98452  247 NVVPAEAMLSYVIRAPKmGAMYEIADRVIKCAQGAAMMTETEMSYKVVsGTSNLMSTKAVLDRFNANLDKYLpIPYTPEE 326 Bacteroides capill...
EDM89115  270 NVVQSRASVLYMVRSNHvAEAVELQKRVDKIAEGAALMTETTYEKKFIdGLADTVSNFALERVLYKNFEELGvPKYTEEE 349 Ruminococcus obeum...
CAJ67127  245 NIVPDFAEVWYYIRGVKmEHVRDVFGRIVDIAKGAALMTGTSMEYDIIsGVYDYIPNTILTDILSQNMKLVGvQNYNEND 324 Clostridium diffic...
CAJ67683  241 NVVPGFAESWYFIRGKKaKDAEHVLDRLIKVAQGAAMMTETEMEYKVTdGIYDYIPNQCLTDLVYDNMVFVGcPKNTPEE 320 Clostridium diffic...
CAJ70005  243 NIVPDKASVWYYTRALSrEAVESTYERLVKVAKGAAMMTETEVEVEFLgGCYNTLNNHVLANLVSECMNEIKqEPWTREE 322 Clostridium diffic...
AAR37815  250 NVVPNTAESFYYVRHPNeNALREIWDRVVATANAAAMGTGTTMDYEVIhGNYSVLPNDTLARVMHANLSRVGgFAYSDAD 329 uncultured marine ...
Feature 1                                                                                     
CAG76983  357 RKMaamfQTALSAEDIAESyarfgvkpqpdl----------tlhdgiyplysPNEAFi-GSTDVGTVSWVVPTVQIRSAT 425 Erwinia carotovora...
NP_420367 421 QAFakavQKNVGSTKQEGLdvklk-----------------------glkppEEKPEsgGSDDIGDISWIMPTITINYPS 477 Caulobacter cresce...
AAK90015  328 IAFaksiQDTFTDEAIKSSirlyqikgdvfsnrki--dgstplhlglrdfegQSHFRa-GSTDVGDVSWVTPTAQCWTPA 404 Agrobacterium tume...
AAC74419  327 LAFakqiQATLTSNDRQNSlnniaatggengkvfalrhretvlanevapyaaTDNVLa-ASTDVGDVSWKLPVAQCFSPC 405 Escherichia coli s...
EDM98452  327 LEYaqkfKDTLDKSDEKRLlhcieltypekssae----iramyeepmanwycPEVGNt-ASTDAGDASWVVPGCQLHVAC 401 Bacteroides capill...
EDM89115  350 NAYadalAETYDSSGVPGVaaendenakeqvekmq-keyghamngfltplyqKDAFKa-GSTDVGDVSWLTPTAQIHVAA 427 Ruminococcus obeum...
CAJ67127  325 YYFadklAETVSIDKRSSVssvlsgnkditkm--------nlhdevtddtftHNNCIs-LSLDIGDVSYIIPTAQCSCSV 395 Clostridium diffic...
CAJ67683  321 EEFakklCDTLTREERLGVatslqndksive----------syihkeivdgdKDKGLa-GSTDVGDVSYVIPVAQFAMAA 389 Clostridium diffic...
CAJ70005  323 LDFaaelDRQSPQQYKAMIskynlpegt---------------hlyygggnvTNFNSy-GSTDIGDVMHIVPTAYFFTGC 386 Clostridium diffic...
AAR37815  330 KVFaneiAKTLPTGAYIDGraigi------------------------kpfeVGQRSn-GSTDVGDVSWTVPTVGMSAAT 384 uncultured marine ...
Feature 1            #                                             
CAG76983  426 YAiGTPAHSWQLVAQGKTGAAHKGMEYAAKAMASLAIDLLATPELIAQAKADH 478 Erwinia carotovora subsp. atroseptica SCRI1043
NP_420367 478 NIpDLPGHHWANAISMATPIAHKGVVAGSKVVAMTTLDLLTQPKLLADSKAYF 530 Caulobacter crescentus CB15
AAK90015  405 WAiGTNPHTWQVVAQGKAPAAHKAMAHAAKAMASTGLALMTSPELLANAKKEW 457 Agrobacterium tumefaciens str. C58
AAC74419  406 FAvGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEH 458 Escherichia coli str. K-12 substr. MG1655
EDM98452  402 YPaGLPFHSWQMVAMGKSSLAKKGMKTAAKVLAMTALDFMTDPALLEQAKKDY 454 Bacteroides capillosus ATCC 29799
EDM89115  428 WPnGCPGHSWQNVSCDRTEIGHKAAVHAGKVIAATAIDLIEDTSLLDEARAEF 480 Ruminococcus obeum ATCC 29174
CAJ67127  396 WPiGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKEL 448 Clostridium difficile 630
CAJ67683  390 WPvGIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEF 442 Clostridium difficile 630
CAJ70005  387 TNlGAPGHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEF 439 Clostridium difficile 630
AAR37815  385 WVpGTGAHTWQAVAAGRTPIGHKGMLVAAKAMALTAVTLFQNKSILVEANAEF 437 uncultured marine bacterium 443

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