1KOL,1JQB,1Y9A,1YKF,2DPH,2NVB


Conserved Protein Domain Family
FDH_like

?
cd05278: FDH_like 
Click on image for an interactive view with Cn3D
Formaldehyde dehydrogenases
Formaldehyde dehydrogenase (FDH) is a member of the zinc-dependent/medium chain alcohol dehydrogenase family. Formaldehyde dehydrogenase (aka ADH3) may be the ancestral form of alcohol dehydrogenase, which evolved to detoxify formaldehyde. This CD contains glutathione dependant FDH, glutathione independent FDH, and related alcohol dehydrogenases. FDH converts formaldehyde and NAD(P) to formate and NAD(P)H. The initial step in this process the spontaneous formation of a S-(hydroxymethyl)glutathione adduct from formaldehyde and glutathione, followed by FDH-mediated oxidation (and detoxification) of the adduct to S-formylglutathione. Unlike typical FDH, Pseudomonas putida aldehyde-dismutating FDH (PFDH) is glutathione-independent. The medium chain alcohol dehydrogenase family (MDR) have a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The N-terminal region typically has an all-beta catalytic domain. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit.
Statistics
?
PSSM-Id: 176181
View PSSM: cd05278
Aligned: 16 rows
Threshold Bit Score: 388.556
Threshold Setting Gi: 27573723
Created: 26-Jul-2005
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:1KOL: Pseudomonas putida formaldehyde dehydrogenase binds NAD, contacts at 4A
    View structure with Cn3D
  • Structure:1YFK: Thermoanaerobacter brockii ADH binds NADP, contacts at 4A
    View structure with Cn3D
  • Structure:2DPH: Pseudomonas putida formaldehyde dismutase, contacts at 4A
    View structure with Cn3D
  • Structure:2NVB: Thermoanaerobacter brockii alcohol dehydrogenase binds NADP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 ##  #                               
1KOL_A      3 NRGVVYLGsgKVEVQKIDYPkmqdprgkkieHGVILKVVSTNICGSDQHMVRGrtt---aqvGLVLGHEITGEVIEKGRD 79  Pseudomonas putida
2DPH_A      3 NKSVVYHGtrDLRVETVPYPklehn-nrkleHAVILKVVSTNICGSDQHIYRGrfi---vpkGHVLGHEITGEVVEKGSD 78  Pseudomonas putida
P77316      1 MKALTYHGphHVQVENVPDPgve------qaDDIILRITATAICGSDLHLYRGkipq--vkhGDIFGHEFMGEVVETGKD 72  Escherichia coli K-12
NP_421633   1 MKALRYHGarDVRYESMDDPtpq------sdRDAIVQVTACSICGSDLHIYHGhgfs--edlGFCVGHEAVGEVVEVGRG 72  Caulobacter cresce...
AAO79617    1 MLAYTYIEhgKFELREKPEPkit------daRDAIVRVTLGSICTSDLHIKHGsvpr--avpGITVGHEMVGVVEEVGAE 72  Bacteroides thetai...
XP_656535   1 MKGLCFSDenQLEIIEVEKPqin------heTDAVVKITFASICSSDIHILKGkvpr--akkGVILGHEGVGEVVEIGKD 72  Entamoeba histolyt...
YP_460312  14 MKAMVYRGpgRYGLEDVPAPvil------epDDVIGRVTLSSISGSDVQIVHGglpe--vrtPLIVGHEFCAEIMEVGPA 85  Syntrophus aciditr...
YP_583462   1 MKAAVYYGpqDIRCTDIPDPvir------sdHEMLVKVTATSICGSDLHLYRGaldgimekgKSQTGHELIGEVVEVGKS 74  Ralstonia metallid...
YP_715012   1 MRATLLEDvgKIALREFPDPvvq------rpTDAVVRVTASCICGSDLHPYRGktp---svfPKRIGHEFVGVVEDIGDE 71  Frankia alni ACN14a
YP_754396   5 MKALVYRGpeKIGLEDVPMPkii------epDDAIVKVTTSTICGTDIHIWHGgmpe--vepGRIIGHEFVGEVVEVGPA 76  Syntrophomonas wol...
Feature 1                  #                                                                  
1KOL_A     80 VeNLQIGDLVSVPFnvaCGRCRSCkemhtGVCLtvnparagga------------ygyvdmgdwtGGQAEYVLVPYAdfn 147 Pseudomonas putida
2DPH_A     79 VeLMDIGDLVSVPFnvaCGRCRNCkearsDVCEnnlvnpdadlg-----------afgfdlkgwsGGQAEYVLVPYAdym 147 Pseudomonas putida
P77316     73 VkNLQKGDRVVIPFviaCGDCFFCrlqqyAACEntnagkgaalnkkqipapaalfgyshlyggvpGGQAEYVRVPKGnvg 152 Escherichia coli K-12
NP_421633  73 VqRLKVGDKVMLPAavgCGRCRSClsgviQTCEngqgacy------------------glsarlqGSQAEAVRVPAAdmn 134 Caulobacter cresce...
AAO79617   73 VtSVRPGDRVTVNVetfCGECFFChhgyvNNCTdpnggwa-------------------lgcridGGQAEYVRVPYAdqg 133 Bacteroides thetai...
XP_656535  73 VkKIKVGDHVSINCitfCGNCWFCqhgyiNNCEyggwei---------------------gcrenGTMAEYVRIPFAdss 131 Entamoeba histolyt...
YP_460312  86 VkSLNVGDRVVVSCvafCGECRSCrqglhARCEktgfgsf-------------------gmngpdGGQAEYVRLPGAdry 146 Syntrophus aciditr...
YP_583462  75 VgRFKQGDRVSMGYsvsCGHCYMCevgqtAHCEttknavygf---------------gipfgsinGTHAEALIVPHAdgh 139 Ralstonia metallid...
YP_715012  72 VtTVRPGDVVVAPFlisDGTCRYCraglhTSCEngsvwgtd----------------dgsgarsdACQGDRIRAPLAdgt 135 Frankia alni ACN14a
YP_754396  77 VrNNKVGDKVAVSCvtqCGECFYCvrgvySHCTtgswif---------------------gymidGCQAEYVRVPHAnlg 135 Syntrophomonas wol...
Feature 1                          #   #                   # ###                  ###   #     
1KOL_A    148 lLKLPDrdkamekirDLTClsDILPTGYHGAVTAgvgpgSTVYVAGAGPVGLAAAASARLLGaAVVIVGDLnpaRLAHAK 227 Pseudomonas putida
2DPH_A    148 lLKFGDkeqamekikDLTLisDILPTGFHGCVSAgvkpgSHVYIAGAGPVGRCAAAGARLLGaACVIVGDQnpeRLKLLS 227 Pseudomonas putida
P77316    153 pFKVPPlls----ddKALFlsDILPTAWQAAKNAqiqqgSSVAVYGAGPVGLLTIACARLLGaEQIFVVDHhpyRLHFAA 228 Escherichia coli K-12
NP_421633 135 aVPVPDgvs----mdQALMmtDALATAWFGARNAevrpgASVAVIGLGPIGLMAVESAYVMGaHVVYAIDPlpaRRALAE 210 Caulobacter cresce...
AAO79617  134 lNRIPDtvs----deQALFvgDVLATGFWATRISeitekDTILLIGAGPTGICTLLCSMLKNpKRIIVCEKspeRIQFVR 209 Bacteroides thetai...
XP_656535 132 lNVIPKevk----deQCLLvgDVLSSGYFGVELGevkegDIVTVIGCGPVGLCSMLCCKMKK-AKVIALDIntqCLEYAQ 206 Entamoeba histolyt...
YP_460312 147 cFKIPEslt----fqDVLFcgDVLSAGYYGAEMGdiqrgETVVVVGAGPVGLCAMASARLREpSRIIAVDTnpyRLDAAL 222 Syntrophus aciditr...
YP_583462 140 aMNVPKgip----deAAVTlsCNLPSAIIANRLAdiqvgENVALVGCGPTGMMTLDIALHRGpGRVVVLDKvahRLDVVR 215 Ralstonia metallid...
YP_715012 136 lVRVGTelpe-allpDVLAlsDVMGTGHHAAVCAgvgpgSTVVVVGDGAVGLCAVLAARRLGaERVVAMSRtpsRQKLAA 214 Frankia alni ACN14a
YP_754396 136 mFTIPDglt----eeDVLFvgDILSTGYFGSEQGriepgDTVAVMGGGPVGMCAMTTARLWGpAEIIAVDInqeRLDFAV 211 Syntrophomonas wol...
Feature 1                                         ###                       #                #
1KOL_A    228 aq--gfEIADLSldtplhEQIAALLge-pEVDCAVDAVGFearghgheg-----akheapatVLNSLMQVTRVAGKIGIP 299 Pseudomonas putida
2DPH_A    228 da--gfETIDLRnsaplrDQIDQILgk-pEVDCGVDAVGFeahglgde------antetpngALNSLFDVVRAGGAIGIP 298 Pseudomonas putida
P77316    229 dry-gaIPINFDedsdpaQSIIEQTaghrGVDAVIDAVGFeakgsttetvltnlklegssgkALRQCIAAVRRGGIVSVP 307 Escherichia coli K-12
NP_421633 211 ea--gaIALHPDea---lARVREDTkg-rGLDCVIEAVGGe--------------------aTVDAALRLVRPRGVVSVI 264 Caulobacter cresce...
AAO79617  210 ehy-pdVLITTPen--ckEFVLQNSdh-sGADVVLEVAGTe--------------------dSFRMAWDCARPNAIVTIV 265 Bacteroides thetai...
XP_656535 207 klhccdWSFNPHncd-ivNEILSITnn-rGCDCTIENAGTn--------------------eSFELSWKLTRPNGIVALV 264 Entamoeba histolyt...
YP_460312 223 kagvadLALNPVkdr-vvEIVQGLTdg-rGADKTIECAGMq--------------------tTFDIAFQAVRGGGKIATA 280 Syntrophus aciditr...
YP_583462 216 kk--ggVAIDANqed-wkEKALAETgg-rGFDKVIEVVGYp--------------------eTLQMCLDLVRPGGTVAAI 271 Ralstonia metallid...
YP_715012 215 efg-atDIVAGRgae-giAQVGELLg--dGADAVLECVGSq--------------------eSLEQAAAVAHPGGRLGCV 270 Frankia alni ACN14a
YP_754396 212 kngiadVGINPQkvgnvgDAIRERTgg-rGADVTIEANGFk--------------------pTFDMAVDSVRPGGTISLI 270 Syntrophomonas wol...
Feature 1      #                                  ###                                         
1KOL_A    300 GLYVtedpgavdaaakigslsirfgLGwAKSHSFHTGQTpv-mkYNRALMQAIMwdrini-aevvgVQVISLDDAPRGYG 377 Pseudomonas putida
2DPH_A    299 GIYVgsdpdpvnkdagsgrlhldfgKMwTKSIRIMTGMApv-tnYNRHLTEAILwdqmpylskvmnIEVITLDQAPDGYA 377 Pseudomonas putida
P77316    308 GVYAgfihg------------flfgDAfDKGLSFKMGQThv-haWLGELLPLIEkgllk--peeivTHYMPFEEAARGYE 372 Escherichia coli K-12
NP_421633 265 GVQQakrfa------------fpleRAfGAGLTFRVGTCsv-peELPALFPLVRsgrlr--pekyiSHRLPLSDGPEAYR 329 Caulobacter cresce...
AAO79617  266 ALYDkpllf------------plpeMY-GKNLTFKTGGVdg--cDCAEILSLIEegkid--ttpliTHRCSLNEIEEAYR 328 Bacteroides thetai...
XP_656535 265 AMYEsnqpf------------plplMY-GKNLIFKTGGVda--iHCDELIEKIKnkeid--tmpllTQQFTFDQILSAFN 327 Entamoeba histolyt...
YP_460312 281 SIFQkpvtv------------pldaAW-GRNISLSWGFApi--dRIPKLIRLIEegkin--tgflcTHQAPLNDILRGYD 343 Syntrophus aciditr...
YP_583462 272 GVFCdstfn------------lnlaDVfLRNISLHMNGFanaqpYMWEALRLMErgvin--pqeyfSHAFELADVDKAFS 337 Ralstonia metallid...
YP_715012 271 GAPLgnl---------------plgPLfMKNVRLAGGVApa-raYLPELLDDVLagrvr--pgrifDLELPLDEVAEGYR 332 Frankia alni ACN14a
YP_754396 271 GVFEkpqel------------amntLW-IKNIRISMGLVna--nRIPELIKLIQrgkid--tnyliTHRAPLNDILKGYD 333 Syntrophomonas wol...
Feature 1      #              
1KOL_A    378 EFDAGvp--kKFVIDP 391 Pseudomonas putida
2DPH_A    378 KFDKGsp--aKFVIDP 391 Pseudomonas putida
P77316    373 IFEKReeecrKVILVP 388 Escherichia coli K-12
NP_421633 330 LFESReagalKMVLTP 345 Caulobacter crescentus CB15
AAO79617  329 IFENKldgviKVAIVC 344 Bacteroides thetaiotaomicron VPI-5482
XP_656535 328 FFQNKpthcvKVVIQF 343 Entamoeba histolytica HM-1:IMSS
YP_460312 344 IFGNRkdgclKWLVTP 359 Syntrophus aciditrophicus SB
YP_583462 338 VFHQKsdsamKVLIRP 353 Ralstonia metallidurans CH34
YP_715012 333 AMDERra--iKVLLRP 346 Frankia alni ACN14a
YP_754396 334 VFGGKkdgclKWVVTP 349 Syntrophomonas wolfei subsp. wolfei str. Goettingen

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap