1MR9,1OCX,1KRV,1KHR


Conserved Protein Domain Family
LbH_MAT_like

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cd04647: LbH_MAT_like 
Click on image for an interactive view with Cn3D
Maltose O-acyltransferase (MAT)-like: This family is composed of maltose O-acetyltransferase, galactoside O-acetyltransferase (GAT), xenobiotic acyltransferase (XAT) and similar proteins. MAT and GAT catalyze the CoA-dependent acetylation of the 6-hydroxyl group of their respective sugar substrates. MAT acetylates maltose and glucose exclusively while GAT specifically acetylates galactopyranosides. XAT catalyzes the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients. Members of this family contain a a left-handed parallel beta-helix (LbH) domain with at least 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). They are trimeric in their active form.
Statistics
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PSSM-Id: 100053
View PSSM: cd04647
Aligned: 191 rows
Threshold Bit Score: 79.8079
Threshold Setting Gi: 118711634
Created: 22-Nov-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of E. coli galactoside acetyltransferase and Enterococcus faecium streptogramin acetyltransferase Vat(D) bound to CoA and their respective substrates.
  • Comment:Active acetyltransferases in this family are trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:1KRV; E. coli Galactoside Acetyltransferase trimer binds three CoA and substrate 4-nitrophenylgalactoside molecules; defined at 4A contacts.
    View structure with Cn3D
  • Structure:1KHR_A; Enterococcus faecium streptogramin acetyltransferase Vat(D) binds CoA and substrate Virginiamycin; defined at 4A contacts. Shown are two active sites within the trimer.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               # #                                             # #                     
1MR9_Z       29 NVEVGeYSYYDskngetfdkQILyhypilndkLKIGKFCSIGpgVTIIXng---aNHRXdgstypfnlfgngw------- 98  Enterococcus fae...
1KRV_C       75 NIHIGrNFYANfn------lTIVdd-----ytVTIGDNVLIApnVTLSVt-----GHPVhhelrkn-------------- 124 Escherichia coli
ZP_00590682  68 YLDIGdHVHIGgg------cHVSgt-----ggVTISDFCGLSqgVRIYSv-----TDDYtgysltnatv----------- 120 Pelodictyon phae...
AAF93428     37 RISIGsNVRIDdy------vTISagv----ggVEIGSHVHIGvySSLIGa-----GKITledfvgvsgrvsiysssddyt 101 Vibrio cholerae ...
NP_978344    35 VISVGnHVRIDdf------cILSg-------kITIGSYSHISayTALYGgevgieMHDFanisaktivyavlddfsg--- 98  Bacillus cereus ...
YP_419475    60 NFVCGkNVWIGeg------aILDam-----ggLEIGDNSQIGsyVMVWSh----sSHKQairgetgt------------- 111 Magnetospirillum...
YP_005844   143 NLELGdDVVVHr-------yVFLddi----ggIKIGDRTSLSdyVNVYSh-----THHVlaspd---------------- 190 Thermus thermoph...
CAI50898    147 NIEMGdNVVVHnd------vLLDdr-----grLQIGDRVSVAdrSHIHTh-----AHDTvdqsd---------------- 194 Natronomonas pha...
YP_610190   216 GIALGeHVGLGrg------vVIEga-----ggVAIGEHSQLDdqVTLVSs-----SHDHgyrsl---------------- 263 Pseudomonas ento...
YP_462640    30 NLKLGyKTDIGaf------tYINak-----ygVTIEDFVQVGshCSIYSv-----STIDdr------------------- 74  Syntrophus acidi...
Feature 1                                   # ##    #           ##    ##        ## #    
1MR9_Z       99 -----ekhxpkldqlpikgDTIIGND-VWIGKdVVIXpgVKIGDgAIVAANSVVVKDIAPyXLAGGNPANEI 164 Enterococcus faecium
1KRV_C      125 -------------gemysfPITIGNN-VWIGShVVINpgVTIGDnSVIGAGSIVTKDIPPnVVAAGVPCRVI 182 Escherichia coli
ZP_00590682 121 ---------perfksvkvaPVNLGRH-VLIGSgSVVLpgVTIGEgSAVGALSLVSISLDEwGVYFGSPAKRI 182 Pelodictyon phaeoclathra...
AAF93428    102 gmamsnptvpeeltkvtslPVLIKKH-SILGAgCVVLpkVIVGEgVSVGALSLVNKSLDDwHIYSGNPIQKF 172 Vibrio cholerae O1 biova...
NP_978344    99 --ntlmgptvpnqyrnvktEKVILKKhAIIGAnSILFpnVTIGEgTAVGAMSMVKESLDDwYIYAGIPVRKV 168 Bacillus cereus ATCC 10987
YP_419475   112 -----------srdrieykRTKIGSN-VHIPGpTVIApgVTIGNgVMIAPLSFVNEDLPDrAVFSPAREMRR 171 Magnetospirillum magneti...
YP_005844   191 ---------------vtlkETIIGSG-VRITYhATVLagVRIGDdAMVGTGAVVTKDIPPhAIALGIPARPV 246 Thermus thermophilus HB27
CAI50898    195 ---------------vtnyETILDDD-VRLGYgSMISagCRIGEnAMVGSGATTLGDVPPhHIAAGSPAKSV 250 Natronomonas pharaonis D...
YP_610190   264 --------------pwkaaPIRIGKR-CRIGTgALIVgpVSLGDdVVVEPYAVVIRDVPAhSVVKGIIQLKE 320 Pseudomonas entomophila L48
YP_462640    75 -----------------qgPVVLKKN-CRIGShSVVMpgVTVGEnAVVGACSFVNADIPEnAVAVGMPARVI 128 Syntrophus aciditrophicu...

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