2CDQ,2J0W


Conserved Protein Domain Family
AAK_AK
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cd04234: AAK_AK 
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AAK_AK: Amino Acid Kinase Superfamily (AAK), Aspartokinase (AK); this CD includes the N-terminal catalytic domain of aspartokinase (4-L-aspartate-4-phosphotransferase;). AK is the first enzyme in the biosynthetic pathway of the aspartate family of amino acids (lysine, threonine, methionine, and isoleucine) and the bacterial cell wall component, meso-diaminopimelate. It also catalyzes the conversion of aspartate and ATP to aspartylphosphate and ADP. One mechanism for the regulation of this pathway is by the production of several isoenzymes of aspartokinase with different repressors and allosteric inhibitors. Pairs of ACT domains are proposed to specifically bind amino acids leading to allosteric regulation of the enzyme. In Escherichia coli, three different aspartokinase isoenzymes are regulated specifically by lysine, methionine, and threonine. AK-HSDHI (ThrA) and AK-HSDHII (MetL) are bifunctional enzymes that consist of an N-terminal AK and a C-terminal homoserine dehydrogenase (HSDH). ThrA and MetL are involved in threonine and methionine biosynthesis, respectively. The third isoenzyme, AKIII (LysC), is monofunctional and is involved in lysine synthesis. The three Bacillus subtilis isoenzymes, AKI (DapG), AKII (LysC), and AKIII (YclM), are feedback-inhibited by meso-diaminopimelate, lysine, and lysine plus threonine, respectively. The E. coli lysine-sensitive AK is described as a homodimer, whereas, the B. subtilis lysine-sensitive AK is described as a heterodimeric complex of alpha- and beta- subunits that are formed from two in-frame overlapping genes. A single AK enzyme type has been described in Pseudomonas, Amycolatopsis, and Corynebacterium. The fungal aspartate pathway is regulated at the AK step, with L-Thr being an allosteric inhibitor of the Saccharomyces cerevisiae AK (Hom3). At least two distinct AK isoenzymes can occur in higher plants, one is a monofunctional lysine-sensitive isoenzyme, which is involved in the overall regulation of the pathway and can be synergistically inhibited by S-adenosylmethionine. The other isoenzyme is a bifunctional, threonine-sensitive AK-HSDH protein. Also included in this CD is the catalytic domain of the Methylomicrobium alcaliphilum ectoine AK, the first enzyme of the ectoine biosynthetic pathway, found in this bacterium, and several other halophilic/halotolerant bacteria.
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Statistics
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PSSM-Id: 239767
Aligned: 141 rows
Threshold Bit Score: 164.954
Created: 26-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:putative catalytic residues [active site]
Evidence:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #                                      #                                    
2CDQ_A       28 TCVMKFGGSSVasaermkEVADliltf-peesPVIVLSAMGkTTNNLLLAGekavscgvsnaseieelsiikelhirtvk 106 thale cress
ZP_01117611   1 MKVLKFGGSSVansenikKVLTivahasqknkVVVIVSAFGkTTDKLLATAnsalqdisisksiletiralhnqiindlv 80  Polaribacter irg...
ZP_00950435   1 MNVLKFGGTSVanaenitKVINilkseakdtqLLAVVSALGgITDVLLEAGelanakdeaylttfdaikarhletvealf 80  Croceibacter atl...
ZP_00307997   1 MKVLKFGGTSVgsvesirKVAEilssykkkekFVVVFSAMSgITNLLVEVGqkasttdisyqqvlktieqkhvstaktli 80  Cytophaga hutchi...
AAO77510      1 MKVMKFGGTSVgsvnsilSVKRives--agepVIVVVSALGgITDKLINTSkmaaagdsayegefreivyrhvemikevi 78  Bacteroides thet...
ZP_01060709   1 MRVLKFGGTSVgsvaninQVINivkngaqdqkIAVVVSALGgITDLLMQAGtdasnkedyntafkdieakhieftrtlip 80  Flavobacterium s...
ZP_01258021   1 MKILKFGGSSIanqlcinNIIDivkn---etkCVVVVSAISgVTNLLNNCMnkakegdlkfkleideifikhldiinlfi 77  Psychroflexus to...
YP_267214    26 IQVHKFGGSSLatpkcikRALEiiren-cqlnDIVVVSANGkTTDRLFALYslvertiaedtiqksdeltqaidelakeq 104 Colwellia psychr...
CAI87770      3 NNIHKFGGSSLssaerykSVAKiiigq-aqpgDCVVVSAAGkTTDTLVKLWqsyqqhdtqaladivlqlnnhqsdlitql 81  Pseudoalteromona...
NP_662905     1 MRVFKFGGSSIasaakisNVAGiirrelkstpLVVVVSAIGkVTDMLAETAalagngdaayrdklegiaslhggiirelf 80  Chlorobium tepid...

Feature 1                                                     #                                 
2CDQ_A      107 eln-----idpsviltyleeleqllkgiammkeltlrtrdYLVSFGECLSTRIFAAYLntigVKARQYDafeigfittdd 181 thale cress
ZP_01117611  81 vvhk---kevskevtylfnrltsicegifllqelsdktlaKVCSFGERLSSLIIANAAke-lFDAAHKEsrdliltnddy 156 Polaribacter irg...
ZP_00950435  81 pehik--ediisevsailerlkdilqgiylinefsfktrdKVLSFGELLSSYIISEALklhvKDAQLKDsreliatdsyf 158 Croceibacter atl...
ZP_00307997  81 dvknq--gkviaqlklilnelqdllhgvyllkelsprsndLVLSFGERLSSYLVSEFLkqegVDVTFLDarkviitdssf 158 Cytophaga hutchi...
AAO77510     79 pagag-qvalqrqigellnelkdifqgiylirdlspktsdTIVSYGERLSSIIVAELI----DEAKWFDsrtfiktekkh 153 Bacteroides thet...
ZP_01060709  81 dsk-----ealdeiknllgnlesllqgiylinelspktvdKLLAYGEILSSSIIARAMyaqgLDAVRKDsrdlittndkh 155 Flavobacterium s...
ZP_01258021  78 skkse--gelitfikneltkaekllrgislvkeitpniysKIIVTGEILSSKLINEIFisekLNSRLVDgkdliyisgdn 155 Psychroflexus to...
YP_267214   105 aqlitellnanntkqlllrlhkdieqlttwltadivqhhnDVLALGEVWSARLLSALLneqvCPSNSIDsrdflvidneq 184 Colwellia psychr...
CAI87770     82 lq--------gdlkrsalttlneelslvaklasteqlqeaALLAHGELWSARLLAAYLqqlnVAACMQDarglftlsdg- 152 Pseudoalteromona...
NP_662905    81 gtea---saeetwlgemmaelndvlhgvfllrelsdkslaLVLSYGERLSCRIVSRYMhvsgTPAECVDarsvivtddnh 157 Chlorobium tepid...

Feature 1                                                            #                          
2CDQ_A      182 ftngdileatypavakRLYDDWMhd-pAVPIVTGFLGkgwktGAVTTLGRGGSDLTATTIGKALGLkeIQVWKDVDGVLt 260 thale cress
ZP_01117611 157 l----naqvnfkltneNIGSFFQenknRVVMLGGFISsn-isGNTTTLGRGGSDFSASIYAAALNAeeLQIWTDVSGMYt 231 Polaribacter irg...
ZP_00950435 159 t----kanvkeeltnqNISSYFKsskaSVTVLPGFISrn-eiGETTTLGRGGSDYTAAILASALNAsqLEIWTDVSGMYt 233 Croceibacter atl...
ZP_00307997 159 g----sakvdfkktdkAIKDYFKdi-kSSVVTTGFIGst-dkGETTTLGRGGSDYTASVFGAALDSegIEIWTDVDGIMt 232 Cytophaga hutchi...
AAO77510    154 n----khtidadltnqLVKEAFHsi-pKVSLVPGFISsdkvsGDVTNLGRGGSDYTAAIIAAALDAssLEIWTDVDGFMt 228 Bacteroides thet...
ZP_01060709 156 t----kagvnykvtnsQVEYYFTkakqKITVLPGFIAst-aqGETTTLGRGGSDFTAAIVAAALDVdqVEIYTDVSGMYt 230 Flavobacterium s...
ZP_01258021 156 q----nhlidwektskKVKQLFNqnsfHINVIPGFICkn-enGDLSTLGRGGSDFSASIIANLLDAksLEIWTDVSGVYt 230 Psychroflexus to...
YP_267214   185 -----scvvdtalsaaQLRQRRQf--gKLAVITGYISkd-srGKSCTLGRNGSDYSATIIASLVAAgnVTLWTDVDGIYs 256 Colwellia psychr...
CAI87770    153 -------qllhsqntqQCSELISq--hKINVVTGFIAan-tqGDTVTLGRNGSDYSATLLASYCNAkqVYIWTDTQGIFs 222 Pseudoalteromona...
NP_662905   158 c----fakvdrlatgkLIHERFRsf-dVLPVVTGFIAsa-pdGSVTNLGRGGSDFTATILGAALHAeeVWIWTDVDGFYs 231 Chlorobium tepid...

Feature 1                         #            #                                  
2CDQ_A      261 CDPTIYkratPVPYLtfdEAAELayfGAQVLHPQSMRPAREg----eIPVRVKNSYNPkapGTIIT 322 thale cress
ZP_01117611 232 ANPSVVkeayPISEIsyeEAMELshfGAKVLYPPTIQPALRk----eIPIRIKNTFNPesiGTLIC 293 Polaribacter irgensii 23-P
ZP_00950435 234 AHPKLVkqafPIAQIsyqEAMELshfGAKVLYPPTIQPVLDk----nIPILIKNTFFPedkGTRIV 295 Croceibacter atlanticus HTCC2559
ZP_00307997 233 ADPRKVkrafTLPQVsyiEAMEMshfGAKVIYPPTLQPAFKk----rIPIWIKNTFNTafeGTLIN 294 Cytophaga hutchinsonii
AAO77510    229 ADPRVIstayTISELsyvEATELcnfGAKVVYPPTIYPVCHk----nIPIIIKNTFNPdgvGTVIK 290 Bacteroides thetaiotaomicron V...
ZP_01060709 231 ANPKMVkqakPIDSIsyhEAMELshfGAKVLYPPTIVPVMSk----nIPIRIKNTLQPedpGTLIH 292 Flavobacterium sp. MED217
ZP_01258021 231 ANPKIVsqarPIKKIsyhEAMELshfGAKVIYPPTVQPLIDk----kIELKIKNTFSPenkGTLIS 292 Psychroflexus torquis ATCC 700755
YP_267214   257 ADPRVVpsarKLHRLpngVAKELgrlGNPVLHAKTLLPLINplsehhTHLHVASSFDAqviGTEIG 322 Colwellia psychrerythraea 34H
CAI87770    223 TDPRKVnkaiKYAKVcraQANLLarlGNPVLHAKTLSPLKNt----dIKLAVRSSFDVqasSTNVV 284 Pseudoalteromonas haloplanktis...
NP_662905   232 ADPKRVpdarVIPEIsyaEAMELshaGAKVLHPLAVQPVMKa----gIPLRIKNSFNPekpGTRIG 293 Chlorobium tepidum TLS

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