Conserved Protein Domain Family
Wrch_1

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cd04130: Wrch_1 
Wnt-1 responsive Cdc42 homolog (Wrch-1) is a Rho family GTPase similar to Cdc42
Wrch-1 (Wnt-1 responsive Cdc42 homolog) is a Rho family GTPase that shares significant sequence and functional similarity with Cdc42. Wrch-1 was first identified in mouse mammary epithelial cells, where its transcription is upregulated in Wnt-1 transformation. Wrch-1 contains N- and C-terminal extensions relative to cdc42, suggesting potential differences in cellular localization and function. The Wrch-1 N-terminal extension contains putative SH3 domain-binding motifs and has been shown to bind the SH3 domain-containing protein Grb2, which increases the level of active Wrch-1 in cells. Unlike Cdc42, which localizes to the cytosol and perinuclear membranes, Wrch-1 localizes extensively with the plasma membrane and endosomes. The membrane association, localization, and biological activity of Wrch-1 indicate an atypical model of regulation distinct from other Rho family GTPases. Most Rho proteins contain a lipid modification site at the C-terminus, with a typical sequence motif CaaX, where a = an aliphatic amino acid and X = any amino acid. Lipid binding is essential for membrane attachment, a key feature of most Rho proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 133330
View PSSM: cd04130
Aligned: 10 rows
Threshold Bit Score: 294.695
Threshold Setting Gi: 66772305
Created: 23-Jun-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Rho molecules assume an active conformation when bound to GTP and inactive when GTP is hydrolyzed to GDP
  • Comment:Mg2+ ion plays a key role in bringing together the functional regions of the phosphate-binding, switches I and II
  • Citation:PMID 9545299

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 ######                                      ## #                       
AAK83340      50 VKCVLVGDGAVGKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQ---------------------- 107 human
CAD48484      39 LKCVLLGDGAVGKTSLVVSYSTNGYPTEYVPTAFDNYSVRIEIDGNALRLQICDTAGQ---------------------- 96  Ciona intestinalis
BAB18640      36 LKCILLGDGAVGKTSLLVSYTTNGYPTRYIPTAFDDFSALVQVEDTPVKLQLCDTAGQ---------------------- 93  African clawed ...
AAL17966      32 IKCVLVGDGAVGKSSLIVSYTCNGYPARYRPTALDTFSVQVLVDGAPVRIELWDTAGQ---------------------- 89  human
NP_505686     10 LKCIFVGNAAVGKTSMIVSYTTNVYPHNYVPTAFDNFSVVVLVDKKPIRLQLHDTAGQ---------------------- 67  Caenorhabditis ...
NP_001007444  47 VKCVLVGDGAVGKTSLIVSYTTNGYPTEYVPTAFDNFAAVVAVDGKPVKLQLCDTAGQ---------------------- 104 zebrafish
AAH77840      32 IKCVLVGDGAVGKTSLVISYTINGYPTEYQPTALDTFSVQVLVDGAPVRIQLCDTAGQ---------------------- 89  African clawed ...
NP_001012250  30 ISCMLVGDGAVGKTSMIVSYTTNGYPTDYKQTAFDVFSGQVQVDGTPVRIQLMDTAGQ---------------------- 87  zebrafish
AAY55464      27 IKCVLVGDGAVGKTNLILSYLENRFNPEHIPTASDIYNADVNVNESPVHLTICDTAGQ---------------------- 84  fruit fly
CAG10657       1 LSCMLVGDGAVGKTSMIISYIFNGYNSNYTQTAFDVFTGLVHVNGVPTRIKLIDSAGQvdlahnvrvslsraypsqlslc 80  Tetraodon nigro...
Feature 1                                                                  # #                   
AAK83340     108 ----DEFDKLRPLCYTNTDIFLLCFSVVSPSSFQNVSEKWVPEIRChcPKAPIILVGTQSDLRedvkvlie-ldkckekP 182 human
CAD48484      97 ----DEFDGMRPLCYPGTDVVLVCFSVVRPTSLCNIRDKWLPEIKKylPKVPVVLVGTQTDLRtsldvlvd-laryserP 171 Ciona intestinalis
BAB18640      94 ----DEFDKLRHFCYPKTDVLILCFSVVSPSSFQNISEKWVSEIQChcPNVPVVLLGTQCDLRedvkvliq-laryqdkP 168 African clawed ...
AAL17966      90 ----EDFDRLRSLCYPDTDVFLACFSVVQPSSFQNITEKWLPEIRThnPQAPVLLVGTQADLRddvnvliqldqggregP 165 human
NP_505686     68 ----SSFDTLRPLCYTDADVFVIVYSVVDLQSFEDVSHHWYPEVTKrnPGTKLILVGTQADQRwqv----------rgnT 133 Caenorhabditis ...
NP_001007444 105 ----DEFDKLRPLCYTNADVFLLCFSVVSPSSFQNVREKWVPEIRRhcPRAPILLVGTQSDLRedvkvliq-laqykerP 179 zebrafish
AAH77840      90 ----EDFDHLRSLCYSETDVFLVCFSVVNPSSFQNVTEKWIPEIRThsPHTPIVLVGTQADLRddvnvlin-lsqshvkP 164 African clawed ...
NP_001012250  88 ----EEFDEFRSLSYAHTDVFLLCFSVVNPTSFQNITKKWIPEIREcnPSSPIILVGTQSDLVldvnvlid-ldrykvkP 162 zebrafish
AAY55464      85 ----DTLDPLRELCYPDSDVFLLCFSVVKPETFRAIKTKWAPKFAK--TKAALILVGTQADLRtspnvlnk-lqtngeeA 157 fruit fly
CAG10657      81 llsqEEFGHLRALCYAHVDVFILCFSLVNPVSFQNIASKWIPQIRSgnRTSPIVLVGTQSDLCqsvdilih-laqhgtkP 159 Tetraodon nigro...
Feature 1                              ##                
AAK83340     183 VPEEAAKLCAEEIKAaSYIECSALTQKNLKEVFDAAIVAG 222 human
CAD48484     172 VTTEEAGAFAKQCGAcGYVECSALTQKNLKQVFDTAILEG 211 Ciona intestinalis
BAB18640     169 VSNSSARALAEKIGAvAYVECSALTQKNLKEVFDTAIVSG 208 African clawed frog
AAL17966     166 VPQPQAQGLAEKIRAcCYLECSALTQKNLKEVFDSAILSA 205 human
NP_505686    134 VTQLRGKALADRLGA-EFFECSALTQHNLKQMFDAAILAG 172 Caenorhabditis elegans
NP_001007444 180 VEPQDASVCAEEVQAvSYMECSALTQKNLKEVFDTAIVAS 219 zebrafish
AAH77840     165 VSESQAQAVAQKIRAqTYIECSALTQKNLKEVFDASILSA 204 African clawed frog
NP_001012250 163 VCSSRARSLSEKIRAaEYVECSALTQKNLKEAFDAAIFAA 202 zebrafish
AAY55464     158 ISYADAWDLATTIGA-KYIETSSATQDKVKDVFDTAIWEG 196 fruit fly
CAG10657     160 VAHGRATRLARRIGAgDYLECSALTQHNLKDVFDSAVFAA 199 Tetraodon nigroviridis

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