1K9I,1DV8,1SL4,1SL5


Conserved Protein Domain Family
CLECT_DC-SIGN_like

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cd03590: CLECT_DC-SIGN_like 
Click on image for an interactive view with Cn3D
C-type lectin-like domain (CTLD) of the type found in human dendritic cell (DC)-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) and the related receptor, DC-SIGN receptor (DC-SIGNR)
CLECT_DC-SIGN_like: C-type lectin-like domain (CTLD) of the type found in human dendritic cell (DC)-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) and the related receptor, DC-SIGN receptor (DC-SIGNR). This group also contains proteins similar to hepatic asialoglycoprotein receptor (ASGP-R) and langerin in human. These proteins are type II membrane proteins with a CTLD ectodomain. CTLD refers to a domain homologous to the carbohydrate-recognition domains (CRDs) of the C-type lectins. DC-SIGN is thought to mediate the initial contact between dendritic cells and resting T cells, and may also mediate the rolling of DCs on epithelium. DC-SIGN and DC-SIGNR bind to oligosaccharides present on human tissues, as well as, on pathogens including parasites, bacteria, and viruses. DC-SIGN and DC-SIGNR bind to HIV enhancing viral infection of T cells. DC-SIGN and DC-SIGNR are homotetrameric, and contain four CTLDs stabilized by a coiled coil of alpha helices. The hepatic ASGP-R is an endocytic recycling receptor which binds and internalizes desialylated glycoproteins having a terminal galactose or N-acetylgalactosamine residues on their N-linked carbohydrate chains, via the clathrin-coated pit mediated endocytic pathway, and delivers them to lysosomes for degradation. It has been proposed that glycoproteins bearing terminal Sia (sialic acid) alpha2, 6GalNAc and Sia alpha2, 6Gal are endogenous ligands for ASGP-R and that ASGP-R participates in regulating the relative concentration of serum glycoproteins bearing alpha 2,6-linked Sia. The human ASGP-R is a hetero-oligomer composed of two subunits, both of which are found within this group. Langerin is expressed in a subset of dendritic leukocytes, the Langerhans cells (LC). Langerin induces the formation of Birbeck Granules (BGs) and associates with these BGs following internalization. Langerin binds, in a calcium-dependent manner, to glyco-conjugates containing mannose and related sugars mediating their uptake and degradation. Langerin molecules oligomerize as trimers with three CTLDs held together by a coiled-coil of alpha helices.
Statistics
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PSSM-Id: 153060
View PSSM: cd03590
Aligned: 36 rows
Threshold Bit Score: 119.717
Threshold Setting Gi: 15281079
Created: 23-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:carbohydrate binding site
Evidence:
  • Structure:1DV8_A, human CLTD of H1 subunit of asialoglycoprotein receptor bound with calcium at site designated #2, contacts at 3.5 A
    View structure with Cn3D
  • Structure:1K9I_A, human DC-SIGN bound with Glcnac2man3, contacts at 3.5 A
    View structure with Cn3D
  • Comment:The carbohydrate cross-links two monomeric CTLDs in the DC-SIGN crystals.
  • Structure:1SL4_A, human DC-SIGN CTLD, bound with Man4, contacts at 3.5 A.
    View structure with Cn3D
  • Structure:1SL5_A, human DC-SIGN CTLD, bound with Lnfp Iii (Dextra L504), contacts at 3.5 A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                          #          
1K9I_A      8 CPWEWTFFQGNCYFMSns-----qRNWHDSITACKevgAQLVVIKSaEEQNFLQlqss-------rsnrFTWMGLSDlnq 75  human
1DV8_A      1 CPVNWVEHERSCYWFSrs-----gKAWADADNYCRledAHLVVVTSwEEQKFVQhhi---------gpvNTWMGLHDq-- 64  human
1SL4_A      7 CPWEWTFFQGNCYFMSns-----qRNWHDSITACKevgAQLVVIKSaEEQNFLQlqss-------rsnrFTWMGLSDlnq 74  human
1SL5_A      7 CPWEWTFFQGNCYFMSns-----qRNWHDSITACKevgAQLVVIKSaEEQNFLQlqss-------rsnrFTWMGLSDlnq 74  human
AAK91849   26 RQTRGYKSLAGCLGHGp-------LVLQLLSFTLL---AGLLVQVS-KVPSSISqeqs-------rsnrFTWMGLSDlnq 87  human
CAG06106   60 FQDGWILFQTSLYYISpe-----eNTWGLSRDFCLqkdADLTVINSkAEQNFAGkf-----------krAVWIGLMEpen 123 Tetraodon nigrovir...
CAG04688   28 CENNWKKFDLGCYFFSkl-----tKNWNQSREFCIskgGDLAVLNSkEEQAFVNgwlk--------tsqNAWIGLFDiet 94  Tetraodon nigrovir...
CAG10613  110 LEQERASLKANYTVLSeaenraikKNWEDSRQDCIrrgADLVVIDRpEEQTFVShtietmvtgkyfwdnSFWIGLKDeev 189 Tetraodon nigrovir...
CAI11792    5 FTDGWFNYQSSFYFISse-----kKNWSESTRNCRdrgADLIIINNkEEQDFVKkis---------ggdVVWIGLSDsde 70  zebrafish
XP_694251 161 YPYEWIYYQSSLYFISse-----sKTWAESRRYCTergADLIIINNrKEQDFVDkit---------ageKAWIGLSDsdv 226 zebrafish
Feature 1                            # #          #     ##        ####           
1K9I_A     76 EGTWQWVDGSPllpsFKQYWnrGEPNNvg------eEDCAEFSGn-----GWNDDKCNLAKFWICKK 131 human
1DV8_A     65 NGPWKWVDGTDye-tGFKNWrpEQPDDwyghglgggEDCAHFTDd----gRWNDDVCQRPYRWVCET 126 human
1SL4_A     75 EGTWQWVDGSPllpsFKQYWnrGEPNNvg------eEDCAEFSGn-----GWNDDKCNLAKFWICKK 130 human
1SL5_A     75 EGTWQWVDGSPllpsFKQYWnrGEPNNvg------eEDCAEFSGn-----GWNDDKCNLAKFWICKK 130 human
AAK91849   88 EGTWQWVDGSPllpsFKQYWnrGEPNNvg------eEDCAEFSGn-----GWNDDKCNLAKFWICKK 143 human
CAG06106  124 DSRFRWVDGTPl---TESYWiqGEPNNyhg----ieEDCVELVSevg-reGWNDLNCSSKNFFLCEK 182 Tetraodon nigroviridis
CAG04688   95 EGTWKWVDGTVv---TTTYWqpGQPNSyg-----gnQDCGEILQdsggvgQWNDDACTADQTWVCEK 153 Tetraodon nigroviridis
CAG10613  190 EGTWVWINNVTe--vEQRYWiqGEPNNygp---stgEDCAAFVNiknprqTWYDASCSEEKHWLCET 251 Tetraodon nigroviridis
CAI11792   71 EGSWKWVDDPSm---TSGFWgtFEPNGkr------gENCAVSRSs-----GWADYPCNNYFQWICEK 123 zebrafish
XP_694251 227 EGSWKWVDDSKp---TSWIWhyGEPSSggg---qveEDCVLTVTs-----AWADYPCDAEFQWICEK 282 zebrafish

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