1BMT


Conserved Protein Domain Family
methionine_synthase_B12_BD

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cd02069: methionine_synthase_B12_BD 
Click on image for an interactive view with Cn3D
B12 binding domain of methionine synthase. This domain binds methylcobalamin, which it uses as an intermediate methyl carrier from methyltetrahydrofolate (CH3H4folate) to homocysteine (Hcy).
Statistics
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PSSM-Id: 239020
Aligned: 22 rows
Threshold Bit Score: 317.669
Created: 12-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
B12 bindingcobalt ligand
Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:B12 binding site [chemical binding site]
Evidence:
  • Structure:1BMT; E.coli methionine synthase with bound B12
  • Citation:PMID 7992050

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                           #   #                #  #                    
1BMT_A         9 VNKRLEYSLVKGitefiEQDTEEARQQatrPIEVIEGPLMDGMNVVGDLFGEGkmfLPQVVKSARVMKQAVAYLEPFIEA 88   Escherichia coli
YP_146569    626 LEERLARYVIEGskdglIPDLEKALETysdPLSIINGPLMAGMDEVGRLFNNNqliVAEVLQSAEVMKAAVAFLEPYMEK 705  Geobacillus ka...
YP_065938    325 PEEGIRKAILKGsr-egLEDTMQILLDqytPLDIINQLLVPSMREVGELFGAGemlLPFVLKSAEIMRASVDILEPYMEK 403  Desulfotalea p...
NP_869107    674 IEERMKHALIKGidkyiVEDTEEARQHydkCLHIIEGPLMAGMSVVGDLFGQGkmfLPQVVKSARVMKKAVAYLEPFMEQ 753  Rhodopirellula...
3122390      644 LFERLAQRIVDGernglDADLDEAMTQk-pPLQIINEHLLAGMKTVGELFGSGqmqLPFVLQSAEVMKAAVAYLEPHMER 722  Mycobacterium ...
NP_484352    638 IEERLKRHIIDGeriglEAQLTKALEQy-pPLEIINTFLLDGMKVVGELFGSGqmqLPFVLQSAETMKAAVAYLEPFMEK 716  Nostoc sp. PCC...
10174247     625 LEERLANYIVEGskdglTEDLDKALAKyddPLDIINGPLMNGMDEVGRLFNNNeliVAEVLQSAEVMKASVAHLEPHMEK 704  Bacillus halod...
NP_442368    638 VEERLKRHIIDGerlglEEALNEALKLy-aPLDIINIYLLDGMKVVGELFGSGqmqLPFVLQSAQTMKAAVAFLEPHMDK 716  Synechocystis ...
NP_294690    668 VQERLRHALVQGvadhvDEDAEAAYQElgsPLAVIEGPLMDGMNVVGDLFGAGkmfLPQVVKSARVMKKAVAYLTPYLEA 747  Deinococcus ra...
ZP_00269222  633 VEERLKQRIVDGdrlglDADLTEAMGKy-pPLEIINSLLLDGMKVVGELFGAGkmqLPFVLQSAETMKAAVAFLEPHMEK 711  Rhodospirillum...
Feature 1                              #######  #                                    ### ###     
1BMT_A        89 Skeqg-----ktnGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGvmvpAEKILRTAKEVNADLIGLSGLITPSLDE 163  Escherichia coli
YP_146569    706 Kegs-------tkGKVILATVKGDVHDIGKNLVDIILSNNGYEVIDLGikvaPQQLIEAVREHQPDIIGLSGLLVKSAQQ 778  Geobacillus ka...
YP_065938    404 Aans-------esTKILLATVQGDVHDIGKNLVNIILSNNGYQVIDIGikasIESIIEAAKRHEVDMICLSGLLVKSAIV 476  Desulfotalea p...
NP_869107    754 EkiesgtqdhkarGKFLIATVKGDVHDIGKNIVGVVLQCNNYEVIDLGvmvsSEKILEEAVKQDADMIGLSGLITPSLDE 833  Rhodopirellula...
3122390      723 Sddd------sgkGRIVLATVKGDVHDIGKNLVDIILSNNGYEVVNIGikqpIATILEVAEDKSADVVGMSGLLVKSTVV 796  Mycobacterium ...
NP_484352    717 Sesgn-----nakGKVIIATVKGDVHDIGKNLVDIILSNNGYKVINLGikqpVENIIEAYNQHKADCIAMSGLLVKSTAF 791  Nostoc sp. PCC...
10174247     705 Kadd------hgkGKIILATVKGDVHDIGKNLVEIILSNNGFRIVNLGikvtSNELIEAVARENPDAIGLSGLLVKSAQQ 778  Bacillus halod...
NP_442368    717 Ddsad-----nakGTFLIATVKGDVHDIGKNLVDIILSNNGYRVVNLGikqpVENIIEAYKKHRPDCIAMSGLLVKSTAF 791  Synechocystis ...
NP_294690    748 Ekaes-----sskGKVLLATVKGDVHDIGKNIVGVVLACNGYQVTDLGvmvpGEKILDEAERLGADVIGLSGLITPSLDE 822  Deinococcus ra...
ZP_00269222  712 Iegq-------ekGIIVLATVKGDVHDIGKNLVDIILSNNGYKVINIGikqpIAAILASARENRADAIGMSGLLVKSTVI 784  Rhodospirillum...
Feature 1                               #                       #  #  #        
1BMT_A       164 MVNVAKEMERQGf--tIPLLIGGATTSKAHTAVKieq--nysGPTVYVQNASrTVGVVAALL 221  Escherichia coli
YP_146569    779 MVVTAQDLRQAGi--sTPILVGGAALTRKFTENKiap--eydGVVLYAKDAMdGLALANQIQ 836  Geobacillus kaustophilus HTA426
YP_065938    477 MQESMPIYREAGl--tQPILLGGAALTKKFVAEAcap--nypSPVVYCQDAFsGLAAIQQHE 534  Desulfotalea psychrophila LSv54
NP_869107    834 MVHVAREMKRIGm--kKPLLVGGATTSAKHTAVRiap--aydGPVLHVLDASrSVGVVEKLL 891  Rhodopirellula baltica SH 1
3122390      797 MKENLEEMNTRGvaekFPVLLGGAALTRSYVENDlae--iyqGEVHYARDAFeGLKLMDTIM 856  Mycobacterium tuberculosis
NP_484352    792 MKENLEVFNEKGi--nVPVILGGAALTPKFVHKDcqn--tykGKVIYGKDAFsDLHFMDKLM 849  Nostoc sp. PCC 7120
10174247     779 MVLTAQDLKQQQi--sIPILVGGAALTRKFTNTKiap--eydGLVVYAKDAMnGLELANKLM 836  Bacillus halodurans C-125
NP_442368    792 MKENLEVFNQEGi--tVPVILGGAALTPKFVHQDcqn--tykGQVIYGKDAFaDLHFMDKLM 849  Synechocystis sp. PCC 6803
NP_294690    823 MVNVAREMTRRGv--kTPLLIGGATTSRAHTAVKidp--aydGTVVHVLDASrAVTVTNDLL 880  Deinococcus radiodurans R1
ZP_00269222  785 MKDNLEEMAREGw--nTPVLLGGAALTRAFVEEDcvsayagsGRVAYARDAFdGLDLMAKVA 844  Rhodospirillum rubrum

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