1FR8,2AE7


Conserved Protein Domain Family
b4GalT

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cd00899: b4GalT 
Click on image for an interactive view with Cn3D
Beta-4-Galactosyltransferase is involved in the formation of the poly-N-acetyllactosamine core structures present in glycoproteins and glycosphingolipids.
Beta-4-Galactosyltransferase transfers galactose from uridine diphosphogalactose to the terminal beta-N-acetylglucosamine residues, hereby forming the poly-N-acetyllactosamine core structures present in glycoproteins and glycosphingolipids. At least seven homologous beta-4-galactosyltransferase isoforms have been identified that use different types of glycoproteins and glycolipids as substrates. Of the seven identified members of the beta-1,4-galactosyltransferase subfamily (beta1,4-Gal-T1 to -T7), b1,4-Gal-T1 is most characterized (biochemically). It is a Golgi-resident type II membrane enzyme with a cytoplasmic domain, membrane spanning region, and a stem region and catalytic domain facing the lumen.
Statistics
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PSSM-Id: 132999
View PSSM: cd00899
Aligned: 30 rows
Threshold Bit Score: 270.222
Threshold Setting Gi: 88193394
Created: 11-Apr-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
ligand bindingmetal binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site
Evidence:
  • Structure:1AE7, human beta1,4-galactosyltransferase complexed with pentasaccharide and trisaccharides; defined by 3.5A.
    View structure with Cn3D
  • Comment:Tyr285 determines the specificity of the enzyme for UDP-Gal

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                # # #                                    #                              
1FR8_A        65 PHKVAIIIPFRNRq--EHLKYWLYYLHPILQrqqLDYGIYVINQAGesmFNRAKLLNVGFKEALKdy------------- 129 cow
Q09323       187 AEKTAIIIPYRNRc--RHLYTLLPNLIPMLMrqnVDFTIFVIEQTTpetFNKGILFNAGYLEALKvd------------- 251 great pond snail
CAA66831     158 RDKIAVIIPYRERq--THLTRLIDFLIPILQrqrLDFRFIVTEQYGndlFNKGRIMNAAFIFAESlg------------- 222 nematode
CAJ77198      64 INRMAVLVPFRDRf--TELQEFIPHLNNFLNnqnVRHTFFIINQVDdlrFNRGALLNVGALESLEaevngiivesitqkn 141 Schistosoma man...
BAC22695      74 VHKMALLVPFRDRf--EELLQFVPHMTAFLKrqgVAHHIFVLNQVDrfrFNRASLINVGFQFASDv-------------- 137 fruit fly
AAH92934      82 PHKMALIVPFRERf--EELLVFVPYMHAFLNkkkIRHKIFIINQVDhfrFNRASLINVGFMESGNd-------------- 145 zebrafish
Q9UBV7        92 PHRLAVLVPFRERf--EELLVFVPHMRRFLSrkkIRHHIYVLNQVDhfrFNRAALINVGFLESSNs-------------- 155 human
AAH84833      88 PHRLALLIPFRERf--EELMSFVPHMHRYLIqkkILHHIFIINQVDhfrFNRASLINVGFLESGNe-------------- 151 African clawed ...
AAW27585     145 ENGVAIIIDIIARdqlKQLYITLSTLIPLLQmqqLCYRIFIIEQINnnvINKGKLKNIGFIEALKyf------------- 211 Schistosoma jap...
NP_001035000  84 PHRLALLVPFRERf--EELLAFVPYMHRFLSkkrIRHHILVLNQVDhfrFNRASLINVGFLESGNd-------------- 147 chicken
Feature 1                             ###                                                        
1FR8_A       130 -------------dyNCFVFSDVDLIPMNDHNTYrc--fsQPRHISVAMd----kFGFSLPYVQYFGGVSALSKQQFLSI 190 cow
Q09323       252 -------------nyDCFILHDVDMIPIDDRNMYrcn-kmGPVHFSPGVn----kFKYKLFYSGLFGGVVGFTREQFRLI 313 great pond snail
CAA66831     223 --------------vDCVVFHDVDMFPQDDRNPYsc--ppGPRHLGAFVs----nLGYQLWYKEIVGGVLAVSMADYRAV 282 nematode
CAJ77198     142 slyqlssqclklpftKYLALHDVDLLPEDPALKYnmpselGPIHLIPFYl-----HPRYYYFKEYAGGVLIIKRTQYTLV 216 Schistosoma man...
BAC22695     138 --------------yDYIAMHDVDLLPLNDNLLYeypsslGPLHIAGPK------LHPKYHYDNFVGGILLVRREHFKQM 197 fruit fly
AAH92934     146 --------------tDYIAMHDVDLLPQNEDLNYgfp-vdGPFHVASPE------LHPLYHYKTYVGGILLLTKKHYLAC 204 zebrafish
Q9UBV7       156 --------------tDYIAMHDVDLLPLNEELDYgfp-eaGPFHVASPE------LHPLYHYKTYVGGILLLSKQHYRLC 214 human
AAH84833     152 --------------tDYIAMHDVDLLPLNLDLDYgfp-dkGPFHVASPE------LHPLYHYKTYVGGILLLTKQHYEMC 210 African clawed ...
AAW27585     212 -------------kfQCVIFHDVDLAPINYTNSYrc--deITKHMVVHLsvgtnvNKFKLPYSTYIGGVLKMSTHHFISV 276 Schistosoma jap...
NP_001035000 148 --------------tDYIAMHDVDLLPLNEQLDYgfp-eaGPFHVASPE------LHPLYHYKTYVGGILLLTKQHYEMC 206 chicken
Feature 1                                                                                        
1FR8_A       191 NGFPNNYWG-WGGEDDDIYNRLAFRgmsVSRPNAv-IGKCRMIRHsrdk------------------------------- 237 cow
Q09323       314 NGASNLYFG-WGGEDDDLRNRAVHMklpLLRKTLa-HGLYDMVSHveagwnvnphskgahslydmlnkalgvqagwnvhp 391 great pond snail
CAA66831     283 NGYSNQFWA-WGGEDDDMGQRILSLnytIERPNPe-TGRYSMLKHvkrk------------------------------- 329 nematode
CAJ77198     217 GGMSNSFWG-WGREDDEFQIRLKLKgfkIVTPINv-TMGLKAFRHihqed------------------------------ 264 Schistosoma man...
BAC22695     198 NGMSNQYWG-WGLEDDEFFVRIRDAglqVTRPQNikTGTNDTFSHihnry------------------------------ 246 fruit fly
AAH92934     205 NGMSNRFWG-WGREDDEFFRRLKAAnleLFRPTGi-TTGTKTFRHihdpa------------------------------ 252 zebrafish
Q9UBV7       215 NGMSNRFWG-WGREDDEFYRRIKGAglqLFRPSGi-TTGYKTFRHlhdpa------------------------------ 262 human
AAH84833     211 NGMSNRFWG-WGREDDEFYRRIKGAeleLFRPTGi-STGYKTFRHihdpa------------------------------ 258 African clawed ...
AAW27585     277 NGYSNKYWSlNNEHNDDFVKRLNATdikYVHVDGa-IGRYIYIPStrpy------------------------------- 324 Schistosoma jap...
NP_001035000 207 NGMSNRFWG-WGREDDEFYRRIKGAglqVHRPSGi-TTGYETFQHlhdpa------------------------------ 254 chicken
Feature 1                                                                                
1FR8_A       238 --------------------knepNPQRFDRIAHTKETMlSDGLNSLTYmv-----lEVQRYP-LYTKITVD 283 cow
Q09323       392 nskwplrlfdsvnhapaegagwnvNPDRFKIYSTSRQRQhVDGINSLVYnv-----tWYRTSP-LYTWVGVG 457 great pond snail
CAA66831     330 ---------------------rtaPKLIYKLLGNSANRVaYDGLNETDKwt----irKVTTRP-LYYHLYVD 375 nematode
CAJ77198     265 -------------------hhkrdVKTYYNPDVVNLIRNpVGGLTSINYtvvkrrliTISSIPaIIINVKLY 317 Schistosoma mansoni
BAC22695     247 -------------------hrkrdTQKCFNQKEMTRKRDhKTGLDNVKFkilkvhemLIDQVPvTILNILLD 299 fruit fly
AAH92934     253 -------------------wrkrdQKRIAAQKQEQFKVDpEGGLSNLRYkvesrkevSISGAPcTVINTFVE 305 zebrafish
Q9UBV7       263 -------------------wrkrdQKRIAAQKQEQFKVDrEGGLNTVKYhvasrtalSVGGAPcTVLNIMLD 315 human
AAH84833     259 -------------------wrkrdQKRIAAQKQEQFKVDrAGGLHSVKYrvesrsevTISGAPcTVLNVLLE 311 African clawed frog
AAW27585     325 ---------------------lsqSINSKQLLTNSVKRMnSDGLNSAAYkv-----vSYKELP-FFTHLLVL 369 Schistosoma japonicum
NP_001035000 255 -------------------wrkrdQKRIAAQKQEQFKVDrEGGLNNVRYriesrtalSVAGAPcTVLNILLD 307 chicken

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