2TPL,1AX4


Conserved Protein Domain Family
Tnase_like

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cd00617: Tnase_like 
Click on image for an interactive view with Cn3D
Tryptophanase family (Tnase). This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD correspond to tryptophanase (Tnase) and tyrosine phenol-lyase (TPL). Tnase and TPL are active as tetramers and catalyze beta-elimination reactions. Tnase catalyzes degradation of L-tryptophan to yield indole, pyruvate and ammonia and TPL catalyzes degradation of L-tyrosine to yield phenol, pyruvate and ammonia.
Statistics
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PSSM-Id: 99741
View PSSM: cd00617
Aligned: 9 rows
Threshold Bit Score: 663.671
Threshold Setting Gi: 20140926
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Comment:PLP cofactor required for activity
  • Structure:2TPL_A; Citrobacter tyrosine phenol-lyase
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
2TPL_B       23 ERLKKMQeagYNTFLLNSKDIYIDLLTDSGTnaMSDKQWAGMMMGDEAyaGSENFYHLERTVqel--------------- 87  Citrobacter freu...
1AX4_A       24 EREAALKeagYNPFLLPSSAVYIDLLTDSGTnaMSDHQWAAMITGDEAyaGSRNYYDLKDKAkel--------------- 88  Proteus vulgaris
Q897C2       26 EREIAMKeagYNTFNLRSEDVYIDLLTDSGTnaMSDAQWAGMMIGDEAyaGSKNWLFLESTIkel--------------- 90  Clostridium tetani
ZP_00109641  34 EREQAIAqagYNTFLLRSEDVYIDLLTDSGTsaMSDYQWAGMMLGDEAyaGSKNFYNLEASIqky--------------- 98  Nostoc punctiforme
NP_810405    24 EREQWIKeahYNVFQLKSEQVYIDLITDSGTgaMSDRQWAGMMLGDESyaGATSFFKLKDTItri--------------- 88  Bacteroides thet...
O30971       23 ERERLARaagYNLFGLHSDDVLIDLLTDSGTgaMSSLQWAAVMQGDESyaGSPSFFRFEAAVqnl--------------- 87  Rhodobacter caps...
Q9YCI2       29 ERLRRLReagWNVFRLRSIDIFIDLLTDSGTgsMSIYQWAALMTGDEAyaGARSWFRFRDAVrdv--------------- 93  Aeropyrum pernix
Q9HMV2       19 DREAALAdagHNVFELASDDVAVDLLTDSGTgtMSNDQWAAMLQGDEAyaGSASFEDLAVAAedv--------------- 83  Halobacterium sp...
NP_602743    28 QRERALKathYNEFAFPAGMLTVDMLSDSGTtaMTDQQWSAMFLGDESygRNKGYYVLLDAMrdcfergdnqkkiinlvr 107 Fusobacterium nu...
Feature 1                                                                                       
2TPL_B       88 -----------------------------fgFKHIVPTHQGRGAENLLSQLAIkp--------gqYVAGNMYFtttrYHQ 130 Citrobacter freu...
1AX4_A       89 -----------------------------fnYDYIIPAHQGRGAENILFPVLLkykqkegkaknpVFISNFHFdttaAHV 139 Proteus vulgaris
Q897C2       91 -----------------------------fgFKHVVPTHQGRGAENLLSSIAIkp--------gqYVAGNMYFtttrYHQ 133 Clostridium tetani
ZP_00109641  99 -----------------------------ygYRHIVPTHQGRGAENILSQILIkp--------gdYIPGNMYFtttrLHQ 141 Nostoc punctiforme
NP_810405    89 -----------------------------tgFEYIIPTHQGRAAENVLFSYLVhe--------gnIVPGNSHFdttkGHI 131 Bacteroides thet...
O30971       88 -----------------------------mpFKHIIPTHQGRAAEAILFSIFGgk--------grRIPSNTHFdttrGNI 130 Rhodobacter caps...
Q9YCI2       94 -----------------------------lgLDLVLPVHQGRAAERILYGELLrrr------narIVPANTHFdtgrAVI 138 Aeropyrum pernix
Q9HMV2       84 -----------------------------mgFQHIIPAHQGRGAENVLYGAVLsa--------gdTVLNNAHFdttrAHV 126 Halobacterium sp...
NP_602743   108 tdcqdiekmmnemylceyegglfnggaaqleRPNAFLMPQGRAAESILFEIVRkilavrepgkvfTIPSNGHFdtteGNI 187 Fusobacterium nu...
Feature 1                                                                                       
2TPL_B      131 EKnGAVFVDIvrdeahd------aglniafKGDIDLKKLQKLIdekgaenIAYICLAVTVnlagGQPVSMANMRAVRELT 204 Citrobacter freu...
1AX4_A      140 ELnGCKAINIvtekafd------setyddwKGDFDIKKLKENIaqhgadnIVAIVSTVTCnsagGQPVSMSNLKEVYEIA 213 Proteus vulgaris
Q897C2      134 EKnGGIFVDIirdeahd------asidipfKGNIDVNKLEKLIeekgaenIAYVCLAVTVnlagGQPVSMANMRAVRELT 207 Clostridium tetani
ZP_00109641 142 ELaGGTFVDViideahd------aqslhpfKGNVDLQKLTDLIervgaerIPYISVAGTVnmagGQPISMANLRAVHQLA 215 Nostoc punctiforme
NP_810405   132 EGrHAIALDCtideakn------tqleipfKGNVDPAKLEKALsey-adrIPFIIVTITNntagGQPVSMQNLREVRAIA 204 Bacteroides thet...
O30971      131 EAsGATGDDLviaegkd------pqnlhpfKGNMDLARLEAYLeah-haeVPLVMITITNnaggGQPVSLANIRAVADLA 203 Rhodobacter caps...
Q9YCI2      139 LNqGGVPLDLpspqasr-------reaypfKGDIDVARLERLLker-srdVAFILLVITNntagGQPVSMDNVKTVRELA 210 Aeropyrum pernix
Q9HMV2      127 AAnDATPVDCpvdgard------pdtdapfKGNFSVERARRVVddvgadaVPVVVLTITNnsmaGQPVSIENTREVAAFA 200 Halobacterium sp...
NP_602743   188 KQmGSVPRNLynkellyevpeggryeknpfKGDMDINKLQQLIdavgienIPMIYTTVTNnticGQAVSMKSIRETAKIA 267 Fusobacterium nu...
Feature 1                                                           #                           
2TPL_B      205 AAHGIKVFYDATRCVENAYfikeqeqgfenksiaeIVHEMFSYADGCTMSGKXDCLVNIGGFLCMNdd-eMFSSAKELVV 283 Citrobacter freu...
1AX4_A      214 KQHGIFVVMDSARFCENAYfikardpkyknatikeVIFDMYKYADALTMSAKXDPLLNIGGLVAIRdneeIFTLARQRCV 293 Proteus vulgaris
Q897C2      208 AKHGIKVFYDATRCVENAYfikeqeegyadvsikdIVHEMFSYSDGATMSGKKDGIVNIGGFLAINde-eLFGKAKEIVV 286 Clostridium tetani
ZP_00109641 216 QTYGIRIILDATRAVENAHfiqqreedyssqaiatILREFCSYTDGCTMSGKKDALVNIGGWLALNdy-nLYEEARNLIV 294 Nostoc punctiforme
NP_810405   205 NKYNKPVLFDSARFAENAYfikmrekgyqdksikeITREMFDLADGMTMSAKKDGIVNMGGFIATRrk-eWYEGAKGFCV 283 Bacteroides thet...
O30971      204 HRYGKPFVIDGCRFAENAWfiktreegqadrsipeIVRDCFAVADGMTMSAKKDAFGNIGGWLALNdd-dLAEEARGHLI 282 Rhodobacter caps...
Q9YCI2      211 DAYGLPLVMDICRFAENAYlvkerdpryrgwsvrdIAREMISYGDHFVMSAKKDGLANIGGFIATRdp-sLYEDLAARVV 289 Aeropyrum pernix
Q9HMV2      201 DDIDATFVIDACRFAENAHfvqqrepgyehdsvaaIAREQLSYADACVMSGKKDGLVNVGGFVGLHddgrLHEQCRQRGI 280 Halobacterium sp...
NP_602743   268 HKYEIPFMLDAARWAENCYfikmneegyrdksiaeIAKEMFSYCDGFTASLKKDGHANMGGILAFRd--kGYFWKKFSDF 345 Fusobacterium nu...
Feature 1                                                                                       
2TPL_B      284 VYEGmpsy-------------------gglaGRDMEAMAIGLREAMqyeyIEHRVKQVRYLGdKLKAAGVPiv-ePVGGH 343 Citrobacter freu...
1AX4_A      294 PMEGfvty-------------------gglaGRDMAAMVQGLEEGTeeeyLHYRIGQVKYLGdRLREAGIPiq-yPTGGH 353 Proteus vulgaris
Q897C2      287 VYEGmpsy-------------------ggmtGRDMQAIAIGFREAMqyeyIEHRIRQVRYLGdRLKEAGVPiv-ePVGGH 346 Clostridium tetani
ZP_00109641 295 IYEGlhty-------------------ggmaGRDMEAMARGIEESVqddhIRARVGQVEYLGqKLLDWGIPiv-vPIGGH 354 Nostoc punctiforme
NP_810405   284 QYEGylty-------------------ggmnGRDMNALAIGLDENTefdnLETRIKQVEYLAqKLDEYEIPyq-rPAGGH 343 Bacteroides thet...
O30971      283 RTEGfpty-------------------gglaGRDLDALAQGLVEIVdedyLRYRIRTHQYIVeRLDAMGVPvv-kPAGGH 342 Rhodobacter caps...
Q9YCI2      290 LEEGyvty-------------------gglaGRDLEAIAQGLREVVeedyLRHRVEQVRYLGeLLSSQGVPiv-ePVGGH 349 Aeropyrum pernix
Q9HMV2      281 LYEGfsty-------------------ggmsGRDMAAFAVGLREAVeppyVAERVAQVQRLAdALTDRDVPiy-qPAGGH 340 Halobacterium sp...
NP_602743   346 NEDGtvktdvgillkvkqissygndsygsmsGRDIMALAAGLYECCnfnyLQERVEQCNYLAeGFYKAGVKgvvlPAGGH 425 Fusobacterium nu...
Feature 1                                                                                       
2TPL_B      344 AVFLDArrfceh-ltqdefPAQSLAASIYvetGVRSMERGiisagrnnvtgehhrpkletVRLTIPRrvYTYAHMDVVAD 422 Citrobacter freu...
1AX4_A      354 AVFVDCkklvpq-ipgdqfPAQAVINALYlesGVRAVEIGsfllgrdpatgeqkhadmefMRLTIARrvYTNDHMDYIAD 432 Proteus vulgaris
Q897C2      347 AVFLDArrfcph-ldqeqfPAQSLAASLYidsGVRSMERGivsagrdvntgenhkpkletVRLTIPRrvYTYKHMDVVAE 425 Clostridium tetani
ZP_00109641 355 AIYLDAkrflpq-ipqdqfPAQRLAAELYleaGIRAMERGivsagrnketgdnyypelelVRLTIPRrvYTQAHMDLTAE 433 Nostoc punctiforme
NP_810405   344 AIFVDAskvlth-vpkeefPAQTLTVELYleaGIRGCEIGyiladrdpithenrfngldlLRLAIPRrvYTDNHMNVIAA 422 Bacteroides thet...
O30971      343 AVFIDArawlsh-ippleyPGQALAVALYeiaGVRSCEIGtamfgrqpdg-sekpaamdlVRLAFPRrtYTQSHADYIVE 420 Rhodobacter caps...
Q9YCI2      350 AVYVDVlealpe-mprshyPADALAAALYlesGVRAVGLGalafareeng-eivypefelLRLAVPRrtYTNSHMEYVAA 427 Aeropyrum pernix
Q9HMV2      341 AVYIDAnaalph-lpreqfPGQAFVCELYregGVRAVELGrfafpd--------tdrrdlVRLALPRrtYGPDHLDHVAD 411 Halobacterium sp...
NP_602743   426 GVYINMdeffdgkrghetfAGEGFSLELIrryGIRVSELGdysmeydlktpeqqaevanvVRFAINRsvYSQEHLDYVIA 505 Fusobacterium nu...
Feature 1                                         
2TPL_B      423 GIIKLyqh-kedIRGLKFIYEPk--qLRFFTARF 453 Citrobacter freundii
1AX4_A      433 ALIGLkek-fatLKGLEFEYEPp--vLRHFTARL 463 Proteus vulgaris
Q897C2      426 SVIHLykh-kedIRPLKFTYEPa--qLRFFTAKF 456 Clostridium tetani
ZP_00109641 434 AVEEVyhn-rdrLRGLKMIYEPk--yLRFFQARF 464 Nostoc punctiforme
NP_810405   423 ALRNVferretiTRGVRIAWEAp--lMRHFTVQL 454 Bacteroides thetaiotaomicron VPI-5482
O30971      421 AFEELaat-kdaLRGYRIVKEPk--lMRHFTCRF 451 Rhodobacter capsulatus
Q9YCI2      428 SLARLlregrrkVKGLRVVKEPrikgIRHFLAEL 461 Aeropyrum pernix
Q9HMV2      412 TAAAVcer-gtdVTGLEIVSEPelteLRHFSAAL 444 Halobacterium sp. NRC-1
NP_602743   506 AVKALyed-resIPNMRIVSGHnl-pMRHFHAFL 537 Fusobacterium nucleatum subsp. nucleatum ATCC 25586

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