pom1 DYRK family cell polarity protein kinase Pom1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: pom1
Official Full Name: DYRK family cell polarity protein kinase Pom1
Primary source: PomBase:SPAC2F7.03c
Locus tag: SPOM_SPAC2F7.03C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
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Genomic context

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Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_003424.3 (534120..537873, complement)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division.
Title: DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division.
This work reveals how Pom1-Cdr2-Wee1 operates in multiprotein clusters at the cortex to promote mitotic entry at a cell size that can be modified by nutrient availability.
Title: Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry.
An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and the Pom1 gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool.
Title: Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.
Ssp1 promotes cell polarity by phosphorylating the activation loop of the MARK/PAR-1 family kinase Kin1. Additional Kin1 substrates for cell polarity and cytokinesis were also phosphorylated by the DYRK family member Pom1.
Title: Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.
distinct levels in Pom1 gradients delineate a medial Cdr2 domain, for cell division placement, and control its activity, for mitotic commitment. [Pom1; Cdr2]
Title: Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.
PKA signalling tunes CLASP's activity to promote Pom1 cell side localization and buffer cell size upon glucose starvation.
Title: PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Results demonstrate that Pom1 auto-phosphorylates intermolecularly, both in vitro and in vivo, which confers robustness to the gradient.
Title: Pom1 gradient buffering through intermolecular auto-phosphorylation.
Cdr2 was the only cell cycle target of Pom1 kinase activity that was identified in cells.
Title: Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
The dual regulation exerted by Pom1 on Cdr2 prevents Cdr2 assembly into stable nodes in the cell tip region where Pom1 concentration is high.
Title: Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
Pom1 phosophorylated the C-terminal domain of Cdr2, and this modification reduced Cdr2-T166 phosphorylation by Ssp1
Title: Dueling kinases regulate cell size at division through the SAD kinase Cdr2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	ISM Inferred from Sequence Model more info
enables phosphatidylinositol phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	EXP Inferred from Experiment more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in activation of bipolar cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of actin cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of bipolar cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic G2 cell size control checkpoint signaling	EXP Inferred from Experiment more info	PubMed
involved_in mitotic G2 cell size control checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic G2 cell size control checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell integrity MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to cell tip	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic cytokinesis, division site positioning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment or maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell cortex of cell tip	EXP Inferred from Experiment more info	PubMed
is_active_in cell cortex of cell tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell division site	EXP Inferred from Experiment more info	PubMed
is_active_in cell division site	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell tip	EXP Inferred from Experiment more info	PubMed
is_active_in cell tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasmic side of plasma membrane, cell tip	EXP Inferred from Experiment more info	PubMed
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
is_active_in lateral cell cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic actomyosin contractile ring, intermediate layer	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	EXP Inferred from Experiment more info	PubMed
is_active_in site of polarized growth	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DYRK family cell polarity protein kinase Pom1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

534120..537873 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001018374.3 → NP_592974.1 DYRK family cell polarity protein kinase Pom1 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_592974.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

Q09690

Conserved Domains (2) summary

smart00220
Location:699 → 995

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14210
Location:685 → 995

PKc_DYRK; Catalytic domain of the protein kinase, Dual-specificity tYrosine-phosphorylated and -Regulated Kinase