CLV3 CLAVATA3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: CLV3
Gene description: CLAVATA3
Primary source: Araport:AT2G27250
Locus tag: AT2G27250
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtCLV3; CLAVATA3; F12K2.17; F12K2_17
Summary: One of the three CLAVATA genes controlling the size of the shoot apical meristem (SAM) in Arabidopsis. Belongs to a large gene family called CLE for CLAVATA3/ESR-related. Encodes a stem cell-specific protein CLV3 presumed to be a precursor of a secreted peptide hormone. The deduced ORF encodes a 96-amino acid protein with an 18-amino acid N-terminal signal peptide. The functional form of CLV3 (MCLV3) was first reported to be a posttranscriptionally modified 12-amino acid peptide, in which two of the three prolines were modified to hydroxyproline (Ito et al., Science 2006, 313:842; Kondo et al., Science 2006, 313:845). Ohyama et al. (2009) later reported that the active mature CLV3 is a 13-amino-acid arabinosylated glycopeptide (Nature Chemical Biology, 5:578). CLV3 binds the ectodomain of the CLAVATA1 (CLV1) receptor-kinase. Regulates shoot and floral meristem development. Required for CLAVATA1 receptor-like kinase assembly into a signaling complex that includes KAPP and a Rho-related protein. It restricts its own domain of expression, the central zone (CZ) of the shoot apical meristem (SAM), by preventing differentiation of peripheral zone cells, which surround the CZ, into CZ cells and restricts overall SAM size by a separate, long-range effect on cell division rate. CLE domain of CLV3 is sufficient for function. Results obtained from whole seedlings challenge the concept that the immune receptor FLS2 perceives the meristematic regulatory peptide CLV3p in mesophyll, seedlings, and SAM cells and that CLV3p contributes to SAM immunity against bacterial infection (PMID:22923673).
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Genomic context

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Location:: chromosome: 2

Exon count:: 3

Sequence:: Chromosome: 2; NC_003071.7 (11664978..11665797, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CLAVATA3 mediated simultaneous control of transcriptional and post-translational processes provides robustness to the WUSCHEL gradient.
Title: CLAVATA3 mediated simultaneous control of transcriptional and post-translational processes provides robustness to the WUSCHEL gradient.
WOX going on: CLE peptides in plant development.
Title: WOX going on: CLE peptides in plant development.
CLV signaling involves the secretion of the CLV3 peptide and its perception by a number of Leucine-Rich-Repeat (LRR) receptors, including the receptor-like kinase CLV1 and the receptor-like protein CLV2 coupled with the CORYNE (CRN) pseudokinase. CLV2, and its maize ortholog FASCIATED EAR2 (FEA2) appear to function in signaling by CLV3 and several related CLV3/EMBRYO-SURROUNDING REGION (CLE) peptide ligands
Title: The CLAVATA receptor FASCIATED EAR2 responds to distinct CLE peptides by signaling through two downstream effectors.
The role of CLV3 signal transduction in regulating stem cell fate.
Title: A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis.
Auxin signaling in stem cells is mediated, at least in part, by AUXIN RESPONSE FACTOR 5/MONOPTEROS (ARF5/MP), which directly represses the transcription of DORNROSCHEN/ENHANCER OF SHOOT REGENERATION 1 (DRN/ESR1). DRN expressed in stem cells positively regulates CLAVATA3 (CLV3) expression and has important meristematic functions.
Title: A Molecular Framework for Auxin-Controlled Homeostasis of Shoot Stem Cells in Arabidopsis.
effect of constitutive expression on cell growth and possible role of cytokinins in leaf size control in transgenic tobacco plants
Title: Effect of constitutive expression of Arabidopsis CLAVATA3 on cell growth and possible role of cytokinins in leaf size control in transgenic tobacco plants.
The hypothesis explains how the WUSCHEL gene product, synthesized basally in the meristem, induces CLAVATA3-expressing stem cells in the meristem apex but, paradoxically, not in the basal domain where WUSCHEL itself is expressed. The answer involves the activity of the small family of HAIRY MERISTEM genes, which prevent the activation of CLAVATA3 and which are expressed basally in the shoot meristem.
Title: HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers.
In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool.
Title: FAR-RED ELONGATED HYPOCOTYL3 activates SEPALLATA2 but inhibits CLAVATA3 to regulate meristem determinacy and maintenance in Arabidopsis.
Dynamic expression reveals a two-step patterning of WUS and CLV3 during axillary shoot meristem formation in Arabidopsis
Title: Dynamic expression reveals a two-step patterning of WUS and CLV3 during axillary shoot meristem formation in Arabidopsis.
structures provide insights about the ligand perception and specific interactions between the CLE peptides and their cognate receptors
Title: Differential CLE peptide perception by plant receptors implicated from structural and functional analyses of TDIF-TDR interactions.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001124926.2

Supported by ESTs:

NM_128283.2

Supported by ESTs:

NM_201812.1

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info
enables receptor serine/threonine kinase binding	IEA Inferred from Electronic Annotation more info
enables receptor serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular component organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within innate immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within maintenance of meristem identity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meristem development	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in pattern recognition receptor signaling pathway	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within regulation of meristem structural organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apoplast	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	ISM Inferred from Sequence Model more info
located_in mitochondrion	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: CLAVATA3

NP_001118398.1

CLAVATA3 (CLV3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_565641.2

CLAVATA3 (CLV3); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_973541.1

CLAVATA3 (CLV3); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).