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    pepP Xaa-Pro aminopeptidase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947385, updated on 4-Feb-2024

    Summary

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    Gene symbol
    pepP
    Gene description
    Xaa-Pro aminopeptidase
    Primary source
    ASAP:ABE-0009549
    Locus tag
    b2908
    See related
    ECOCYC:EG10697
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2903
    Summary
    Overexpression causes abnormal biofilm architecture. [More information is available at EcoGene: EG10697]. Aminopeptidase P is an exopeptidase that cleaves the amino-terminal residue from polypeptides, as long as the following residue is a proline . [More information is available at EcoCyc: EG10697].
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    Genomic context

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    Sequence:
    NC_000913.3 (3053515..3054840, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YqfI Neighboring gene 2-octaprenylphenol 6-hydroxylase Neighboring gene 2-octaprenyl-6-methoxyphenol 4-hydroxylase Neighboring gene PF03695 family protein YgfB Neighboring gene cell division protein ZapA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Thioproline formation as a driver of formaldehyde toxicity in Escherichia coli. Patterson JA, et al. Biochem J, 2020 May 15. PMID 32301498
    2. Evaluation of organophosphorus chemicals-degrading enzymes: a comparison of Escherichia coli and human cytosolic aminopeptidase P. Hsu YT, et al. Chem Biodivers, 2008 Jul. PMID 18649306
    3. Complexes of mutants of Escherichia coli aminopeptidase P and the tripeptide substrate ValProLeu. Graham SC, et al. Arch Biochem Biophys, 2008 Jan 15. PMID 17983589
    4. Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P. Implications for metal binding and protein stability. Liu JW, et al. FEBS J, 2007 Sep. PMID 17714507
    5. Tyrosine 387 and arginine 404 are critical in the hydrolytic mechanism of Escherichia coli aminopeptidase P. Jao SC, et al. Biochemistry, 2006 Feb 14. PMID 16460001

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Thioproline formation as a driver of formaldehyde toxicity in Escherichia coli.
      Title: Thioproline formation as a driver of formaldehyde toxicity in Escherichia coli.
    2. analysis of mutants of Escherichia coli aminopeptidase P and the tripeptide substrate ValProLeu
      Title: Complexes of mutants of Escherichia coli aminopeptidase P and the tripeptide substrate ValProLeu.
    3. Mutation of the conserved active site residues His243 and His361 revealed that both are required for catalysis.
      Title: Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: insights into substrate recognition and the mechanism of catalysis.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning and expression of aminopeptidase P gene from Escherichia coli HB101 and characterization of expressed enzyme.
    5. x-ray crystallographic structure of aminopeptidase P in complex with the inhibitor apstatin
      Title: Structure of Escherichia coli aminopeptidase P in complex with the inhibitor apstatin.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables aminopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables aminopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aminopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables manganese ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables manganese ion binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloaminopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloexopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metalloexopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metallopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transition metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proteolysis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Xaa-Pro aminopeptidase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3053515..3054840 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417384.1 Xaa-Pro aminopeptidase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417384.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AFD5
      Conserved Domains (1) summary
      PRK10879
      Location:4 → 441
      PRK10879; proline aminopeptidase P II; Provisional
    Nucleotide Protein
    Heading Accession and Version

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