ABF2 DNA-binding protein ABF2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ABF2
Gene description: DNA-binding protein ABF2
Primary source: SGD:S000004676
Locus tag: YMR072W
See related: AllianceGenome:SGD:S000004676
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA binding activity, bending. Involved in mitochondrial DNA packaging; mitochondrial genome maintenance; and mitochondrion inheritance. Located in mitochondrial chromosome. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis; congestive heart failure; and syndromic microphthalmia 13. Orthologous to several human genes including HMGB1 (high mobility group box 1); HMGB2 (high mobility group box 2); and HMGB3 (high mobility group box 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (411569..412120)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.
Title: Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.
Abf2p does not significantly affect either binding of Cce1p to branched DNA or rate and specificity of Holliday junction resolution. These data suggest the existence of an alternative homologous recombination pathway in yeast mitochondria.
Title: Interaction between Saccharomyces cerevisiae Mitochondrial DNA-Binding Protein Abf2p and Cce1p Resolvase.
results suggest in log-phase cells, lack of Abf2p influences morphology of mt-nucleoids and their transmission into the bud; results suggest in stationary-phase cells, lack of Abf2p does not significantly alter protein composition of the mt-nucleoids
Title: Morphology and protein composition of the mitochondrial nucleoids in yeast cells lacking Abf2p, a high mobility group protein.
Loss of Abf2p, an abundant mitochondrial nucleoid-associated protein, results in increased mitochondrial frameshifts and direct-repeat mediated deletions but has no effect on the rate of mitochondrial point mutations.
Title: Loss of the mitochondrial nucleoid protein, Abf2p, destabilizes repetitive DNA in the yeast mitochondrial genome.
Abf2 binds to most of the mitochondrial genome with a preference for GC-rich gene sequences. Thus, Abf2 is a bona fide mitochondrial DNA-packaging protein in vivo.
Title: Mitochondrial nucleoids undergo remodeling in response to metabolic cues.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding, bending	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial chromosome packaging	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial genome maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial nucleoid	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding protein ABF2

NP_013788.1

Mitochondrial DNA-binding and packaging factor; mtDNA maintenance factor involved in mitochondrial DNA replication and recombination, regulating mtDNA copy number in opposition to DPI34; HMG1 DNA-binding protein family member containing two HMG-boxes; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

411569..412120

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182570.1 → NP_013788.1 TPA: DNA-binding protein ABF2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013788.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZP6, Q02486, Q712M5

UniProtKB/TrEMBL

A0A6A5PPL4, A6ZMD1, B3LLW5, B5VPJ8, C7GL52, C8ZES4, G2WKC0, N1P091

Conserved Domains (1) summary

COG5648
Location:1 → 183

NHP6B; Chromatin-associated proteins containing the HMG domain [Chromatin structure and dynamics]