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    MRF1 Mrf1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852734, updated on 13-Apr-2024

    Summary

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    Gene symbol
    MRF1
    Gene description
    Mrf1p
    Primary source
    SGD:S000003111
    Locus tag
    YGL143C
    See related
    AllianceGenome:SGD:S000003111
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables translation release factor activity. Involved in mitochondrial translational termination. Located in mitochondrion. Orthologous to several human genes including MTRF1L (mitochondrial translation release factor 1 like). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (234717..235958, complement)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Tip20p Neighboring gene putative lipase ROG1 Neighboring gene putative glycosylphosphatidylinositol-alpha 1,2 mannosyltransferase Neighboring gene ubiquitin-ubiquitin ligase HUL5

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mitochondrial release factor in yeast: interplay of functional domains. Kutner J, et al. Curr Genet, 2008 Mar. PMID 18224323
    2. Expression of mitochondrial release factor in relation to respiratory competence in yeast. Towpik J, et al. Curr Genet, 2005 Aug. PMID 16021449
    3. Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1. Claisse ML, et al. Acta Biochim Pol, 2005. PMID 15827612
    4. Mutations in the yeast mrf1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. Towpik J, et al. J Biol Chem, 2004 Apr 2. PMID 14734569
    5. Functional characterization of yeast mitochondrial release factor 1. Askarian-Amiri ME, et al. J Biol Chem, 2000 Jun 9. PMID 10748224

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. mutation in MRF1 that inhibits translation is due to ribosome stalling at the stop codon, and the observed elevated level of release factor is a secondary effect of respiratory deficiency
      Title: Expression of mitochondrial release factor in relation to respiratory competence in yeast.
    2. the natural substrate of the methyltransferase YDR140w is the ternary complex eRF1.eRF3.GTP
      Title: The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene.
    3. Mtq1p methylates Mrf1p A tryptic peptide of Mrf1p, GGQHVNTTDSAVR, containing the GGQ motif was found to be approximately 50% methylated at the glutamine residue in the normal strain but completely unmodified in the peptide from mtq1-Delta.
      Title: The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables translation release factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation release factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables translation release factor activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mitochondrial translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitochondrial translational termination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitochondrial translational termination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitochondrial translational termination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational termination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Mrf1p
    NP_011372.3
    • Mitochondrial translation release factor RF1; involved in stop codon recognition and hydrolysis of peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      234717..235958 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181008.3 → NP_011372.3  TPA: Mrf1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011372.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VU06, P30775
      UniProtKB/TrEMBL
      A0A8H4C2K5, A6ZU40, B3LHJ8, B5VII7, C7GPR0, C8Z8A7, G2WDW0, N1P2Y3
      Conserved Domains (1) summary
      COG0216
      Location:41 → 412
      PrfA; Protein chain release factor A [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P30775.1

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