PEX5 Pex5p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PEX5
Gene description: Pex5p
Primary source: SGD:S000002652
Locus tag: YDR244W
See related: AllianceGenome:SGD:S000002652
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAS10
Summary: Enables peroxisome matrix targeting signal-1 binding activity and protein-macromolecule adaptor activity. Involved in protein import into peroxisome matrix, docking. Located in cytosol and peroxisomal membrane. Part of peroxisomal importomer complex. Human ortholog(s) of this gene implicated in peroxisome biogenesis disorder 2A; peroxisome biogenesis disorder 2B; and rhizomelic chondrodysplasia punctata type 5. Orthologous to several human genes including PEX5 (peroxisomal biogenesis factor 5). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (950563..952401)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Uncovering targeting priority to yeast peroxisomes using an in-cell competition assay.
Title: Uncovering targeting priority to yeast peroxisomes using an in-cell competition assay.
The Ile264 amino acid residue in Pex5, which is adjacent to the FxxxW diaromatic domain that was previously shown to be crucial for recognizing AOx and Cat2 as cargos, is also important for the efficient import of Fox2 and Cta1.
Title: The budding yeast Pex5p receptor directs Fox2p and Cta1p into peroxisomes via its N-terminal region near the FxxxW domain.
Pex5p recognition by the ATPases associated with various cellular activities complex (AAA) depends on the presence of the ubiquitin moiety and is mediated by Pex1p.
Title: Receptor recognition by the peroxisomal AAA complex depends on the presence of the ubiquitin moiety and is mediated by Pex1p.
Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation.
Title: Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation.
Surface plasmon resonance analysis revealed a bivalent interaction mode for the phosphorylated PTS1 receptor Pex5 and Pcs60.
Title: Structural insights into cargo recognition by the yeast PTS1 receptor.
The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor (Pex5).
Title: The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor.
The membrane-associated import receptor Pex5p together with its docking partner Pex14p forms a gated ion-conducting channel which can be opened to a diameter of about 9 nm by the cytosolic receptor-cargo complex.
Title: The peroxisomal importomer constitutes a large and highly dynamic pore.
Pex2 mediates the Ubc4-dependent polyubiquitination whereas Pex12 facilitates the Pex4-dependent monoubiquitination of Pex5.
Title: Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import.
The RING domain of Pex10p exhibits E(3) ligase activity in vitro, in combination with the human E(2) enzyme UbcH5a, a homologue of ScUbc4p, but not when ScPex4p is used as an E(2) enzyme in the reaction.
Title: Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p.
E2D1/2/3 (UbcH5a/b/c) are the mammalian functional counterparts of yeast/plant Pex4p
Title: Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables peroxisome matrix targeting signal-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables peroxisome matrix targeting signal-1 binding	IDA Inferred from Direct Assay more info	PubMed
enables peroxisome matrix targeting signal-1 binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein carrier chaperone	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in protein import into peroxisome matrix	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into peroxisome matrix	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein import into peroxisome matrix, docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into peroxisome matrix, docking	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into peroxisome matrix, docking	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
part_of peroxisomal importomer complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in peroxisomal matrix	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal matrix	IEA Inferred from Electronic Annotation more info
is_active_in peroxisomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal membrane	IPI Inferred from Physical Interaction more info	PubMed
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pex5p

NP_010530.1

Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

950563..952401

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180552.1 → NP_010530.1 TPA: Pex5p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010530.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSM4, P35056

UniProtKB/TrEMBL

A0A7I9DLW6, A6ZYJ6, B3LG45, B5VGD0, C7GTH9, C8Z5F5, G2WAY7, N1PAG2

Conserved Domains (3) summary

PTZ00341
Location:193 → 314

PTZ00341; Ring-infected erythrocyte surface antigen; Provisional

TIGR02917
Location:272 → 544

PEP_TPR_lipo; putative PEP-CTERM system TPR-repeat lipoprotein

sd00006
Location:457 → 485

TPR; TPR repeat [structural motif]