HAC1 transcription factor HAC1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: HAC1
Gene description: transcription factor HAC1
Primary source: FungiDB:YFL031W
Locus tag: YFL031W
See related: SGD:S000001863; AllianceGenome:SGD:S000001863
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ERN4; IRE15
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Involved in negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle and positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response. Located in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See HAC1 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 2

Sequence:: Chromosome: VI; NC_001138.5 (75179..76147)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Sequential Recruitments of Rab-GTPase Ypt1p and the NNS Complex onto pre-HAC1 mRNA Promote Its Nuclear Degradation in Baker's Yeast.
Title: The Sequential Recruitments of Rab-GTPase Ypt1p and the NNS Complex onto pre-HAC1 mRNA Promote Its Nuclear Degradation in Baker's Yeast.
The cap-proximal RNA secondary structure inhibits preinitiation complex formation on HAC1 mRNA.
Title: The cap-proximal RNA secondary structure inhibits preinitiation complex formation on HAC1 mRNA.
Detecting the Non-conventional mRNA Splicing and Translational Activation of HAC1 in Budding Yeast.
Title: Detecting the Non-conventional mRNA Splicing and Translational Activation of HAC1 in Budding Yeast.
Phosphorylation of Pal2 by the protein kinases Kin1 and Kin2 modulates HAC1 mRNA splicing in the unfolded protein response in yeast.
Title: Phosphorylation of Pal2 by the protein kinases Kin1 and Kin2 modulates HAC1 mRNA splicing in the unfolded protein response in yeast.
Vps34 and TOR Kinases Coordinate HAC1 mRNA Translation in the Presence or Absence of Ire1-Dependent Splicing.
Title: Vps34 and TOR Kinases Coordinate HAC1 mRNA Translation in the Presence or Absence of Ire1-Dependent Splicing.
Expression and function of an Hac1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae.
Title: Expression and function of an Hac1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae.
The authors show that phosphorylation of two different HAC1 splicing intermediates is required for their degradation by the 5'-->3' exonuclease Xrn1 to enact opposing effects on the UPR. We also found that ligated but 2'-phosphorylated HAC1 mRNA is cleaved, yielding a decay intermediate with both 5'- and 2'-phosphates at its 5'-end that inhibit 5'-->3' decay and suggesting that Ire1 degrades incompletely processed HAC1.
Title: Multiple decay events target HAC1 mRNA during splicing to regulate the unfolded protein response.
Endoplasmic reticulum stress causes a diminished mRNA decay, thus resulting in the production of a population with an increased abundance of pre-HAC1 mRNA carrying an intact bipartite element, which facilitates its more efficient recruitment to Ire1p foci.
Title: Nuclear mRNA degradation tunes the gain of the unfolded protein response in Saccharomyces cerevisiae.
The consensus RNA structure of the Hac1 mRNA defines the unconventional splice sites and it is well conserved in Fungi and Metazoa
Title: Conserved RNA structures in the non-canonical Hac1/Xbp1 intron.
Here, the authors show that a combination of inhibited translation initiation and accelerated protein degradation-both dependent on the intron-prevents the accumulation of Hac1 protein when the unfolded protein response is inactive.
Title: The fail-safe mechanism of post-transcriptional silencing of unspliced HAC1 mRNA.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to unfolded protein	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription factor HAC1

NP_116622.1

Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates unfolded protein response via UPRE binding and membrane biogenesis; translation initiation repressed under non-stress conditions by un-spliced intron that interacts with 5'-UTR; under ER stress Ire1p nuclease cleaves out inhibitory intron and triggers efficient Hac1p translation; two functional forms of Hac1p are produced; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

75179..76147

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179935.1 → NP_116622.1 TPA: transcription factor HAC1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116622.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTJ9, P41546, P87040

UniProtKB/TrEMBL

A7A208, G2WDB0

Conserved Domains (1) summary

cd14710
Location:41 → 91

bZIP_HAC1-like; Basic leucine zipper (bZIP) domain of Fungal HAC1-like transcription factors: a DNA-binding and dimerization domain