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    rapZ RNase adaptor protein RapZ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947727, updated on 6-Jun-2024

    Summary

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    Gene symbol
    rapZ
    Gene description
    RNase adaptor protein RapZ
    Primary source
    ASAP:ABE-0010522
    Locus tag
    b3205
    See related
    ECOCYC:EG12146
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3194; yhbJ
    Summary
    Unprocessed glmZ upregulates glmS mRNA, so yhbJ mediates the negative feedback regulation of glmS, through glmZ, by the GlmS product glucosamine-6-phosphate. [More information is available at EcoGene: EG12146]. YhbJ appears to regulate the expression of GlmS by controlling the processing and stability of the small RNA regulator GlmZ, possibly in response to the intracellular concentration of the product of GlmS, glucosamine-6-phosphate . [More information is available at EcoCyc: EG12146].
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    Genomic context

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    Sequence:
    NC_000913.3 (3347115..3347969)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribosome hibernation-promoting factor Neighboring gene phosphotransferase system enzyme IIA(Ntr) Neighboring gene phosphorelay protein NPr Neighboring gene protein kinase-like domain-containing protein YrbL Neighboring gene peptidoglycan glycosyltransferase MtgA Neighboring gene low activity glyoxalase ElbB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli. Zhou Y, et al. mSphere, 2021 Mar 3. PMID 33658278, Free PMC Article
    2. Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA. Göpel Y, et al. Genes Dev, 2013 Mar 1. PMID 23475961, Free PMC Article
    3. Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. Resch M, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013 Feb 1. PMID 23385747, Free PMC Article
    4. Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis. Islam MS, et al. EMBO J, 2023 Jan 16. PMID 36504162, Free PMC Article
    5. Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli. Khan MA, et al. EMBO J, 2020 Mar 16. PMID 32065419, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
      Title: Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
    2. A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli.
      Title: A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli.
    3. Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.
      Title: Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.
    4. accumulation of GlmZ* prevents complete GlmZ turnover. This mechanism may serve to adjust a robust glmS basal expression level that is buffered against fluctuations in RapZ availability.
      Title: Feedback regulation of small RNA processing by the cleavage product.
    5. data supports a model in which RapZ forms a complex with GlmZ that interacts with RNase E to guide specific cleavage of the sRNA, thereby down-regulating glmS activity
      Title: Structural insights into RapZ-mediated regulation of bacterial amino-sugar metabolism.
    6. The biological function of GlmZ is to positively control the glmS mRNA in response to GlcN-6-P concentrations and that YhbJ negatively regulates GlmZ. As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite.
      Title: Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables carbohydrate derivative binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables molecular adaptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within RNA destabilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein homotetramerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    RNase adaptor protein RapZ

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3347115..3347969
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417672.1 RNase adaptor protein RapZ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417672.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A377AF68
      Conserved Domains (1) summary
      pfam03668
      Location:1 → 282
      ATP_bind_2; P-loop ATPase protein family
    Nucleotide Protein
    Heading Accession and Version

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    Other Literature Sources
    Molecular Biology Databases
    Research Materials