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    slt soluble lytic murein transglycosylase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948908, updated on 4-Apr-2024

    Summary

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    Gene symbol
    slt
    Gene description
    soluble lytic murein transglycosylase
    Primary source
    ASAP:ABE-0014404
    Locus tag
    b4392
    See related
    ECOCYC:EG10950
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4384; sltY
    Summary
    Slt cleaves the glycosidic bonds between the N-acetylmuramic acid and N-acetylglucosamine residues in murein. [More information is available at EcoGene: EG10950]. Slt70 is involved in growth and recycling of peptidoglycan by catalyzing the lysis of the -1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine, producing 1,6-anhydromuropeptides at an optimal pH of 4.5 with a Km of 200 mg/L . [More information is available at EcoCyc: EG10950].
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    Genomic context

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    Sequence:
    NC_000913.3 (4630733..4632670)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor/NMN adenylyltransferase NadR Neighboring gene energy-dependent translational throttle protein EttA Neighboring gene DNA-binding transcriptional repressor TrpR Neighboring gene inosine/xanthosine triphosphatase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Beta-lactam antibiotics induce a lethal malfunctioning of the bacterial cell wall synthesis machinery. Cho H, et al. Cell, 2014 Dec 4. PMID 25480295, Free PMC Article
    2. High resolution crystal structures of the Escherichia coli lytic transglycosylase Slt70 and its complex with a peptidoglycan fragment. van Asselt EJ, et al. J Mol Biol, 1999 Aug 27. PMID 10452894
    3. Doughnut-shaped structure of a bacterial muramidase revealed by X-ray crystallography. Thunnissen AM, et al. Nature, 1994 Feb 24. PMID 8107871
    4. Exoenzymatic activity of transglycosylase isolated from Escherichia coli. Beachey EH, et al. Eur J Biochem, 1981 May 15. PMID 7018908
    5. Crystallization of the soluble lytic transglycosylase from Escherichia coli K12. Rozeboom HJ, et al. J Mol Biol, 1990 Apr 20. PMID 2184239

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Murein-metabolizing enzymes from Escherichia coli: sequence analysis and controlled overexpression of the slt gene, which encodes the soluble lytic transglycosylase.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lytic endotransglycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lytic transglycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lytic transglycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within peptidoglycan catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in peptidoglycan metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in peptidoglycan-based cell wall IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    soluble lytic murein transglycosylase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4630733..4632670
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418809.4 soluble lytic murein transglycosylase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418809.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376ZMZ2, A0A7A2XMD5, A0A8I0CGC3, A0A8T5YEM3
      Conserved Domains (1) summary
      PRK11619
      Location:1 → 645
      PRK11619; lytic murein transglycosylase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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