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    fepA ferric enterobactin outer membrane transporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945193, updated on 6-Jun-2024

    Summary

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    Gene symbol
    fepA
    Gene description
    ferric enterobactin outer membrane transporter
    Primary source
    ASAP:ABE-0002014
    Locus tag
    b0584
    See related
    ECOCYC:EG10293
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cbr; cbt; ECK0576; fep; feuB
    Summary
    FepA is a protein involved with transport of enterobactin-iron across the outer membrane . [More information is available at EcoCyc: EG10293].
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    Genomic context

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    Sequence:
    NC_000913.3 (610254..612494, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene IS186/IS421 family transposase Neighboring gene phosphopantetheinyl transferase EntD Neighboring gene ferric enterobactin esterase Neighboring gene enterobactin biosynthesis protein YbdZ Neighboring gene apo-serine activating enzyme

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Iron uptake in colicin B-resistant mutants of Escherichia coli K-12. Pugsley AP, et al. J Bacteriol, 1976 Jun. PMID 7543, Free PMC Article

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Colicin-Mediated Transport of DNA through the Iron Transporter FepA.
      Title: Colicin-Mediated Transport of DNA through the Iron Transporter FepA.
    2. Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport.
      Title: Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport.
    3. A stem-loop within E. coli fepA mRNA coding region activates translation initiation.
      Title: Stem-Loop Structures within mRNA Coding Sequences Activate Translation Initiation and Mediate Control by Small Regulatory RNAs.
    4. TonB and FepA display confined mobility within Escherichia coli cell and inner membranes.
      Title: Confined Mobility of TonB and FepA in Escherichia coli Membranes.
    5. The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box.
      Title: Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo.
    6. When FepA binds to ferric enterobactin in outer bacterial membrane, it was reflected by conformational motion of the external vestibular loops.
      Title: Concerted loop motion triggers induced fit of FepA to ferric enterobactin.
    7. the E. coli FepA ortholog (EfeO) cannot replace FepA in FepB-driven iron release from heme indicating protein specificity in these activities
      Title: Staphylococcus aureus FepA and FepB proteins drive heme iron utilization in Escherichia coli.
    8. analysis of the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA
      Title: Direct measurements of the outer membrane stage of ferric enterobactin transport: postuptake binding.
    9. Modification of FepA by fluorescein maleimide was temperature-dependent, and was inhibited by colicin B binding to FepA.
      Title: Fluoresceination of FepA during colicin B killing: effects of temperature, toxin and TonB.
    10. N-terminus verified by Edman degradation on mature peptide
      Title: Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables colicin transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables colicin transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables colicin transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables enterobactin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enterobactin transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ferric-enterobactin transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ferric-enterobactin transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ligand-gated channel activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables siderophore uptake transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables siderophore-iron transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables signaling receptor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within colicin transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within colicin transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in enterobactin transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within ferric-enterobactin import into cell IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within ferric-enterobactin import into cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular iron ion homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in iron ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in siderophore transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in siderophore transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in siderophore-dependent iron import into cell IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within siderophore-dependent iron import into cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cell outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of transmembrane transporter complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ferric enterobactin outer membrane transporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      610254..612494 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415116.1 ferric enterobactin outer membrane transporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415116.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A793MCE0
      Conserved Domains (1) summary
      PRK13524
      Location:1 → 746
      PRK13524; FepA family TonB-dependent siderophore receptor
    Nucleotide Protein
    Heading Accession and Version

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