SCS2 phosphatidylinositol-binding protein SCS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SCS2
Gene description: phosphatidylinositol-binding protein SCS2
Primary source: FungiDB:YER120W
Locus tag: YER120W
See related: SGD:S000000922; AllianceGenome:SGD:S000000922; VEuPathDB:YER120W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables FFAT motif binding activity and phosphatidylinositol binding activity. Involved in several processes, including endoplasmic reticulum organization; regulation of intracellular transport; and regulation of phosphatidylinositol dephosphorylation. Located in several cellular components, including cellular bud tip; nuclear membrane; and nucleus-vacuole junction. Is integral component of endoplasmic reticulum membrane. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis; amyotrophic lateral sclerosis type 8; autosomal dominant adult-onset proximal spinal muscular atrophy; and spinal muscular atrophy. Orthologous to human VAPA (VAMP associated protein A) and VAPB (VAMP associated protein B and C). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SCS2 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (401135..401869)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs.
Title: ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs.
Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150(Glued) subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22
Title: Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1.
Data suggest that a specific pool of phosphatidic acid, associated with lipid droplet formation in perinuclear ER, is responsible for initial rapid exit of Opi1p from nucleus to ER and is required for Ino1 expression in presence of choline; interaction between Opi1p and Scs2p in ER membrane facilitates transit of phosphatidic acid from ER to mitochondria.
Title: Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential for INO1 gene expression in the presence of choline.
The deletion of SCS2 did not affect Ca2+ influx activity when calcineurin was inhibited by FK506, but enhanced this activity by 35% when the enzyme was not inhibited. However, this enhancement was canceled by the deletion of ECM7. These results suggest that Cch1/Mid1 is regulated differentially by Ecm7 and Scs2 in a manner that is dependent on the phosphorylation status of Cch1.
Title: Genetic analysis of the regulation of the voltage-gated calcium channel homolog Cch1 by the γ subunit homolog Ecm7 and cortical ER protein Scs2 in yeast.
this study examined the effects of Scs2p levels on the association of Opi1p with the nuclear membrane by both morphologically and biochemically.
Title: Induction of intranuclear membranes by overproduction of Opi1p and Scs2p, regulators for yeast phospholipid biosynthesis, suggests a mechanism for Opi1p nuclear translocation.
Study shows that a direct interaction between the endoplasmic reticulum (ER) protein Scs2 and the septin Shs1 creates the diffusion barrier in yeast. Barrier formation requires Epo1, a novel ER-associated subunit of the polarisome that interacts with Scs2 and Shs1, encoded by the ORF YMR124W.
Title: Polarization of the endoplasmic reticulum by ER-septin tethering.
model predicts that this process involves an acceptor membrane complex containing the C2 domains of Psd2p, PstB2p, and Pbi1p that ligate to Scs2p and phosphatidic acid present in the donor membrane
Title: An assembly of proteins and lipid domains regulates transport of phosphatidylserine to phosphatidylserine decarboxylase 2 in Saccharomyces cerevisiae.
A prominent ~50-kDa sumoylated protein accumulates in a Ulp1 coiled-coil domain mutant; that was identified as Scs2, an endoplasmic reticulum (ER) membrane protein that regulates phosphatidylinositol synthesis and lipid trafficking.
Title: Desumoylation of the endoplasmic reticulum membrane VAP family protein Scs2 by Ulp1 and SUMO regulation of the inositol synthesis pathway.
show that the Yet1p-Yet3p complex acts in derepression of INO1 through physical association with Scs2p-Opi1p
Title: Yet1p-Yet3p interacts with Scs2p-Opi1p to regulate ER localization of the Opi1p repressor.
three conserved prolines in VAPA and Scs2p confers less vulnerability to mutations equivalent to the amyotrophic lateral sclerosis causing mutation as compared with VAPB, which has only two conserved prolines.
Title: Human VAPA and the yeast VAP Scs2p with an altered proline distribution can phenocopy amyotrophic lateral sclerosis-associated VAPB(P56S).

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables FFAT motif binding	IBA Inferred from Biological aspect of Ancestor more info
enables FFAT motif binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables FFAT motif binding	IPI Inferred from Physical Interaction more info	PubMed
enables phosphatidylinositol binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in endoplasmic reticulum inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endoplasmic reticulum inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum membrane organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum membrane organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endoplasmic reticulum polarization	IDA Inferred from Direct Assay more info	PubMed
involved_in endoplasmic reticulum-plasma membrane tethering	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein import into nucleus	IPI Inferred from Physical Interaction more info	PubMed
involved_in phospholipid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular lipid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of phosphatidylinositol dephosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within reticulophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in subtelomeric heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IPI Inferred from Physical Interaction more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus-vacuole junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphatidylinositol-binding protein SCS2

NP_011046.3

Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

401135..401869

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179010.3 → NP_011046.3 TPA: phosphatidylinositol-binding protein SCS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011046.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM26, P40075

UniProtKB/TrEMBL

A0A6A5Q2D1, A6ZR75, B3LRN6, B5VHP6, C7GKW7, C8Z7D7, G2WD04, N1PA55

Conserved Domains (1) summary

COG5066
Location:2 → 244

SCS2; VAMP-associated protein involved in inositol metabolism [Intracellular trafficking and secretion]