PMC

WT and TLR2^−/− mice infused with porcine T/HS and T/SS lymph for 3 hr. A) Lung permeability to EBD was performed. Data expressed as mean ± SE (n = 4–6 mice/ group). B) MPO levels (U/g) were measured in lung homogenates. Data expressed as mean ± SE (n = 5–6 mice/group).

A) WT and Myd88^−/− and B) WT and TRIF^mut mice were infused with porcine T/SS and T/HS lymph for 3 hr and lung permeability to EBD was performed. Data expressed as mean ± SE (n = 5–8 mice/ group).

WT and TLR4^mut mice were infused with porcine T/SS and T/HS lymph for 3 hr. A) Lung permeability to EBD was performed. Data expressed as mean ± SE (n = 3–12 mice/ group). B) MPO levels (U/g) were measured in lung homogenates. Data expressed as mean ± SE (n = 4–7 mice/group).

A) and B) Western blot of iNOS in lung WCEs of WT and TLR4^mut mice infused with porcine T/SS and T/HS lymph for 3 hr. B) Densitometry was performed to quantify iNOS and total p42/p44 MAPK expression. Data expressed as mean ± SE (n = 4–7 mice/group).

Lung permeability to Evans Blue dye (EBD) was measured in CD-1 mice subjected to actual T/HS or T/SS for 60 min and 3 hr reperfusion as well as CD1 mice infused with porcine T/SS or T/HS lymph for 3 hr. Data expressed as mean ± SE (n = 5–6 mice/ group).

A) Pre- and post-polymyxin-immobilized column eluted T/SS and T/HS lymph samples were infused in naïve C57BL6 mice for 3 hours. Lung permeability to EBD. Data expressed as mean ± SE (n = 3–9 mice/ group). B) DNA isolated from lymph pooled from 3 pigs subjected to either T/HS or T/SS lymph was tested for bacterial DNA contaminants by PCR using 16S rDNA primers. DNA isolated from E.Coli served as our positive control was depicted as + and the negative control containing no DNA template was depicted as -. M denotes a 100 bp size marker.