PMC

Examples of methylation of the MED1 promoter in normal colon mucosa and matched colon adenocarcinoma. Methylation of CpG sites 14 and 16 (arrows) is indicated by preservation of cytosine nucleotides in PCR sequences of both normal mucosa and tumor from the same patient.

Schematic of MED1 promoter with transcription start site indicated by rightward arrow. Enlarged sequence represents amplified region of promoter with vertical hash marks signifying individual CpG sites. Preferentially methylated sites 14 and 16 are in bold.

Relative expression of MED1 in morphologically normal mucosa from patients with polyps or cancer (N=39) compared to MED1 expression in mucosa from non-cancer control patients. (− = polyp, ● = stage I, ■ = stage II, ◆ = stage III, ▲ = stage IV)

Relative expression of MED1 in adenomatous polyp or colorectal cancer specimens (N=39) compared to MED1 expression in matched normal mucosa from the same individual. (− = polyp, ● = stage I, ■ = stage II, ◆ = stage III, ▲= stage IV)