PMC

Alignment of the N-Terminal PCNA/RPA Interaction Domain of MSH6, MSH7, UNG2, and Human p21 Proteins as in .
Mm, Mus musculus.

SDS-PAGE Analysis of Human and Arabidopsis Cosynthesis Reaction Mixtures Used.
Lane 1 contains AtMSH2 and AtMSH3; lane 2, AtMSH2 and AtMSH6; lane 3, AtMSH2 and AtMSH7; and lane 4, hMSH2 and hMSH6. Numbers at the right denote molecular mass.

Gel Filtration Chromatography Analysis of Arabidopsis ³⁵S-Labeled Proteins.
Shown are the elution profiles and the corresponding SDS-PAGE autoradiographs of the eluted fractions for AtMSH2 (open squares), AtMSH7 (open diamonds), and AtMSH2•AtMSH7 (closed circles) synthesis mixtures. See for details.

Gel Filtration Chromatography Analysis of Arabidopsis ³⁵S-Labeled Proteins.
Shown are the elution profiles and the corresponding SDS-PAGE autoradiographs of the eluted fractions for AtMSH2 (open squares), AtMSH3 (open triangles), and AtMSH2•AtMSH3 (closed circles) synthesis mixtures. See for details.

K_av versus Log Molecular Mass Plot of Standards and MSH Polypeptides Analyzed by Gel Filtration Chromatography.
Open circles denote globular standards used to calibrate the gel filtration column. Black diamonds denote MSH polypeptides used in this study. The predicted (theoretical) molecular mass is shown in parentheses to the right of each monomeric or heterodimeric polypeptide.

Representative Mobility Shift Assays of Cosynthesis Reaction Mixtures of Human and Arabidopsis Polypeptides.
Cosynthesis mixtures were incubated with ³²P-labeled homoduplex DNA (T/A, lane 1, all panels), or heteroduplex DNA (T/G, C/C, +1 IDL [insertion–deletion DNA with one T nucleotide looped out], and +3 IDL [insertion–deletion DNA with AAG nucleotides looped out], lanes 2 to 5, respectively, all panels) and analyzed on nondenaturing PAGE (see Methods).
(A) Representative mobility shift assay of hMSH2•hMSH6 cosynthesis mixtures.
(B) Representative mobility shift assay of AtMSH2•AtMSH6 cosynthesis mixtures.
(C) Representative mobility shift assay of AtMSH2•AtMSH7 cosynthesis mixtures.
(D) Representative mobility shift assay of AtMSH2•AtMSH3 cosynthesis mixtures.

Alignment of MSH6-like Protein Sequences.
Black boxes highlight identical amino acids and gray boxes highlight similar amino acids in at least three of the sequences, based on Dayhoff's PAM250 matrix. Dashes denote gaps. The sequence prefixes At-, Hs-, Sc-, and Zm- denote Arabidopsis thaliana, Homo sapiens, Saccharomyces cerevisiae, and Zea mays, respectively. Amino acid positions are shown at right. The lines above the alignment denote conserved regions found in MSH proteins: line 1, the putative N-terminal PCNA/RPA interaction domain; line 2, the N-terminal mismatch recognition domain; line 3, the middle-conserved domain; and line 4, the highly conserved C-terminal domain.

Gel Filtration Chromatography Analysis of Arabidopsis ³⁵S-Labeled Proteins.
Fifty-microliter synthesis mixture samples were layered onto the gel filtration column, fractionated, and analyzed by liquid scintillation and SDS-PAGE (see Methods).
(Top) Elution profiles for AtMSH2 (open squares), AtMSH6 (open triangles), and AtMSH2•AtMSH6 (closed circles) synthesis mixtures. Fractions 32 to 59 for each of the three mixtures are shown (elution profile).
(Bottom) Corresponding SDS-PAGE autoradiographs of the eluted fractions (even numbers 34 to 56). A small amount of the original transcription–translation synthesis reaction mixture is shown in the leftmost lane at bottom (IVTT lane). Arrowheads to the left of the gel panels denote the position expected for each polypeptide. Arrowheads in outline designate theoretical positions of polypeptides not actually present.

Neighbor-Joining Tree for Dayhoff PAM Distances among a Representative Set of Complete MutS/MSH Protein Sequences.
Gaps and regions of ambiguous alignment were excluded from the analysis. Numbers above each branch represent the number of times the branch was found in 100 bootstrap replicas. The Gram-positive Bacillus subtilis and Streptococcus pneumoniae MutS protein sequences were used as an outgroup. All eukaryotic MutS homologs are encoded by the nuclear genome except for the mitochondrially encoded SgMSH1. At, A. thaliana; Av, Azotobacter vinelandii; Bs, B. subtilis; Ec, E. coli; Hi, Haemophilus influenzae; Hs, H. sapiens; Rp, Rickettsia prowazekii; Sc, S. cerevisiae; Sg, Sarcophytom glaucum; Sp (SW14), Saccharomyces pombe; Sp (MutS), S. pneumoniae; Ss, Synechocystis sp; Ta, Thermus aquaticus; Xl, Xenopus leavis; Zm, Z. mays.