PMC

Test hoods versus control hoods. This figure shows the average sash heights of the test and control groups, as recorded every 15 min (lighter colored lines), overlaid with the weekly average sash heights of both groups (bold). After the installation of the MASH alarms, the sash heights of the control group remained relatively unchanged, but the test group exhibited a significant drop in average sash heights. (A value of 0.07 or below corresponds to a closed fume hood sash in the KGS Clockworks system).

SASH (B6.Cg-Kit^W-sh/HNihrJaeBsmJ) mice develop spontaneous airspace enlargement when compared with C57BL/6J mice. SASH mice have a sequence inversion in the 5′ regulatory region of Kit, and lack KIT mRNA expression in the lung. Hematoxylin and eosin images were captured at ×10 magnification for (A) 4-week C57BL/6J, (B) 4-week SASH, (C) 10-week C57BL/6J, (D) 10-week SASH, (E) 14-week C57BL/6J, and (F) 14-week SASH mice. No difference was noted between lung histological sections obtained at 4 weeks between (A) C57BL/6J and (B) SASH mice. By 10 weeks, (C) C57BL/6J mouse lung developed normally whereas (D) SASH mouse lung began to show signs of airspace enlargement. Histology at 14 weeks revealed that (E) C57BL/6J lung had intact parenchyma and well-preserved alveoli whereas (F) SASH lung showed enlarged (emphysema-like) airspaces.

Summary of results. Average sash heights are shown before and after MASH installation, with error margins based off of a 95% confidence interval. The average sash height decreased among all groups. With the 95% confidence interval, the test group’s average sash height decreased with statistical significance (described in the , Section ).

Mast cell deficient and AZ10606120 alleviated high concentrations of ATP-induced inflammation pain. (A) Compared with C57/BL mice, Sash mice alleviated the paw swelling. (B) Compared with the C57/BL mice, the infiltration of inflammatory cells in Sash mice was significantly reduced. (a-b, HE staining of saline and ATP groups in C57/BL mice; c-d, HE staining of saline and ATP groups in Sash mice, 250X). (C) Compared with C57BL mice, Sash mice significantly reduced paw thickening (*p < 0.05, **p < 0.01, WT group vs Sash group, n=8 and 6 respectively). (D) High concentrations of ATP induced mast cells degranulation (The construction of Mrgprb2-Cre tdT +mice was to integrate the fluorescent protein of Td/Tomato into the promoter of MrgprB2, red represented mast cells, and the small red granules around mast cells represented degranulation). (E) Sash mice alleviated the mechanical hyperalgesia induced by high concentrations of ATP (*p < 0.05, WT group vs Sash group, n=8 and 6 respectively). (F) Mast cell deficient attenuated the upregulation of IL-1β and CCL3 (**p < 0.01, ***p < 0.001, saline group vs ATP group, ^#p < 0.05, ^##p < 0.01, WT (ATP) group vs Sash (ATP) group). (G) The infiltration of mast cells expressing P2X7R was significantly increased, which could be inhibited by Z10606120. (H) AZ10606120 significantly relieved the mechanical hyperalgesia. (*p < 0.05, **p < 0.01, ATP group  vs ATP+AZ10606120 group). (I) AZ10606120 attenuated the up-regulation of IL-6 and CCL3 (*p < 0.05, ***p < 0.001, saline group vs ATP group, ^#p < 0.05, ^###p < 0.001, WT (ATP) group vs WT (AZ+ATP) group). (Statistical analysis of the results was performed one-way ANOVA analysis followed by Dunnett’s multiple comparisons test or Tukey’s multiple comparisons test).