PMC

Paired endogenous DNA contents in tooth cementum, petrous bone and parietal bone from the same skeleton, using molecular tooth preservation as condition. A) Poor molecular tooth preservation (<10% endogenous DNA), and B) Good molecular tooth preservation (>10% endogenous DNA) respectively.

Figure 1. Histologic lesions of the rat small bowel corresponding to the different groups in this study. (A) Normal mucosa, 0 minutes of intestinal cold ischemia. (B) Extended subepithelial space along villi; representative of cold ischemia preconditioning + NAME + cold ischemia and cold ischemia + NAME (90 minutes of preservation) and preconditioning + transplant and transplant + NONOS (90 minutes of preservation followed by transplantation with 60 minutes of reperfusion). (C) Subepithelial space at the tip of some villi; preconditioning + cold ischemia and cold ischemia + NONOS (90 minutes of preservation). (D) Epithelial lifting along villus; cold ischemia and cold ischemia + NAME (4 hours of preservation) and transplant and transplant + NAME (90 minutes of preservation followed by a transplantation with 60 minutes of reperfusion). (E) Extended subepithelial space; preconditioning + cold ischemia and cold ischemia + NONOS (4 hours of preservation). (F) Denuded villi; preconditioning + NAME + cold ischemia (4 hours of preservation) and preconditioning + NAME + transplant (90 minutes of preservation followed by transplant with 60 minutes of reperfusion). Hematoxylin and eosin stain, ×127.

(A) Overall methods for preservation and preparation of cell sheets for analysis. hOEC sheets were cultured for 14 days and then preserved for 7 days at 4 °C. After preservation, hOEC sheets were harvested and divided for each analysis. (B) Harvesting of hOEC sheets before and after preservation. (C) Total cell numbers after preservation vs before preservation. The total cell numbers after preservation were calculated as the ratio (%) relative to the number of cells counted before preservation. *P < 0.05 (within a medium with or without ebselen [ebs]). Data represent the mean ± SD of three independent experiments. (D) Viability of cell sheets before and after preservation. *P < 0.05, **P < 0.001 (within a medium with or without ebselen). Data represent mean ± SD of three independent experiments.

Consumer’ preference for food product preservation methods (PRE—addition of preservatives; IRR—irradiation preservation; RWV—radio wave preservation; MAP—packaging in modified atmosphere preservation; STE—sterilisation; PAS—pasteurisation; HPP—high pressure processing; MWV—microwave preservation).