PMC

The effects of flavone A and flavone B on the more differentiated pancreatic cancer Panc28 (A), and the poorly differentiated pancreatic cancer MIA PaCa cells (B) were determined by MTT assay and are represented as a percent of the control absorbance at a wavelength of 570 nm. All data were collected at 24 h after treatment. Data shown are from representative experiments (mean ± SE, n = 3). * p<0.05, significant difference between control and other concentrations for each flavone. # p<0.05, significant difference between flavone A and flavone B treated cells at similar concentrations. C. Half maximal effective concentration (EC₅₀) ± SE for flavone A and flavone B treatment on Panc28 and MIA PaCa cells. The values were estimated by non-linear regression analysis. D. Representative phase contrast images of pancreatic cancer Panc28 and MIA PaCa cells (E.) 24 hours after treatment with flavone A and flavone B at 5, 10, 20, 40 and 80 µM, or treated with vehicle (0 µM of flavone A or B).

The effects of flavone A and flavone B on the more differentiated colon cancer Caco-2 cells (A), and the poorly differentiated colon cancer HCT-116 cells (B) were determined by MTT assay and are represented as a percent of the control absorbance at a wavelength of 570 nm. All data were collected at 24 h after treatment. Data shown are from representative experiments (n = 3). Values are expressed as mean ± SE, * p<0.05, significant difference between control and other concentrations for each flavone. # p<0.05, significant difference between flavone A and flavone B treated cells at similar concentrations. C. Half maximal effective concentration (EC₅₀) ± SE for flavone A and flavone B treatment on colon carcinoma and cells. The values were estimated by non-linear regression analysis. D. Representative phase contrast images of colon carcinoma Caco-2 cells and colon carcinoma HCT-116 (E.) cells, 24 hours after treatment with flavone A and flavone B at 5, 10, 20, 40 and 80 µM, or treated with vehicle (0 µM of flavone A or B).

The effects of flavone A and flavone B on the more differentiated breast cancer MCF7 (A), and the poorly differentiated breast cancer Sk-BR3 cells (B) were determined by MTT assay and are represented as a percent of the control absorbance at a wavelength of 570 nm. All data were collected at 24 h after treatment. Data shown are from representative experiments (mean ± SE, n = 3). * p<0.05, significant difference between control and other concentrations for each flavone. # p<0.05, significant difference between flavone A and flavone B treated cells at similar concentrations. C. Half maximal effective concentration (EC₅₀) ± SE for flavone A and flavone B treatment on MCF7 and SK-BR3 cells. The values were estimated by non-linear regression analysis.

The effects of flavone A and flavone B on the more differentiated prostate cancer LNCaP (A), and the less differentiated PC3 cancer cells (B) were determined by MTT assay and are represented as a percent of the control absorbance at a wavelength of 570 nm. All data were collected at 24 h after treatment. Data shown are from representative experiments (mean ± SE, n = 3). * p<0.05, significant difference between control and other concentrations for each flavone. # p<0.05, significant difference between flavone A and flavone B treated cells at similar concentrations. In the same manner as described above, the effects of flavone A and flavone B normal human colon fibroblasts (C) were determined by MTT assay.