PMC

1: HN37, root sample, two continuous cropping years; 2: HN37, root sample, three continuous cropping years; 3: HN44, root sample, two continuous cropping years; 4: HN44, root sample, three continuous cropping years; 5: HN48, root sample, two continuous cropping years; 6: HN48, root sample, three continuous cropping years; 7: HN37, soil sample, two continuous cropping years; 8: HN37, soil sample, there continuous cropping years; 9: HN44, soil sample, two continuous cropping years; 10: HN44, soil sample, three continuous cropping years; 11: HN48, soil sample, two continuous cropping years; 12 : HN48, soil sample, three continuous cropping years.

Shared and unique OTUs in each sample were estimated at 97% similarity. The numbers of OTUs are indicated inside the diagrams. R-F: rotation soil; S-F-5: soil of potato continuous cropping for 5 years; S-F-10: soil of potato continuous cropping for 10 years; S-F-30: soil of potato continuous cropping for 30 years.

A zoomed-in schematic of the area outlined in and corresponding experimental microphotographs showing the operation of the user-loaded SlipChip (see ). (A) The top plate (contoured in black) consisted of an outlet channel, a reagent inlet, a sample inlet aligned to sample ducts, and reagent wells. (B) The bottom plate (contoured in red) consisted of an outlet aligned with reagent ducts and sample wells. (C) The top plate and bottom plate were assembled and filled with fluorocarbon to generate a SlipChip ready for use. In this orientation, a continuous fluidic path was formed by the reagent inlet, the reagent wells, and the outlet. (D) A reagent was introduced by pipetting. The reagent (yellow) flowed through the continuous fluidic path and filled the reagent wells. A microphotograph below the schematic shows brown dye solution being loaded as the reagent. (E) The chip was slipped into a second position. In this second position, a continuous fluidic path was formed by the sample inlet, the sample ducts, and the sample wells. The sample (green) was introduced by pipetting. The sample flowed through the continuous fluidic path and filled the sample wells. A microphotograph below the schematic shows green dye solution being loaded as the sample. (F) The chip was slipped again into the third position, where the reagent wells were aligned on top of the sample wells, and the sample and reagent in the aligned wells combined by diffusion (dark green). A microphotograph below the schematic shows the reagent combining with the sample in each well, forming individual experiments. The colors of the reagent and the sample in D-F are not consistent for the schematics and the microphotographs. The colors in the schematic were changed to better illustrate the operation of the SlipChip.

Each color represents the percentage of each phylum in each sample. The abscissa represents different treatments, and the ordinate represents percentage of phylum. R-F: rotation soil; S-F-5: soil of potato continuous cropping for 5 years; S-F-10: soil of potato continuous cropping for 10 years; S-F-30: soil of potato continuous cropping for 30 years.