PMC

The hydrolysis was conducted at 50°C for 36 h. 0.06 FPU/gds C: 0.06 FPU/gds of Cellulase; 0.6 FPU/gds C: 0.6 FPU/gds of Cellulase; 0.06 FPU/gds C-EX: 0.06 FPU/gds of Cellulase added with 0.5 µg EXLX1; 0.6 FPU/gds C-EX: 0.06 FPU/gds of Cellulase added with 5 µg EXLX1; 0.06 FPU/gds C-BSA: 0.06 FPU/gds of Cellulase added with 0.5 µg BSA; 0.6 FPU/gds C- BSA: 0.06 FPU/gds of Cellulase added with 5 µg BSA.

Hydrolysis of rice straw and wheat bran. a Hydrolysis of rice straw and wheat bran for the detection of amount of sugar released with time by using commercial cellulase ONZUKAR, cellulase obtained from Schizophyllum commune NAIMCC-F-03379, xylanase obtained from A. oryzae and combination of cellulase and xylanase. b Analysis of hydrolysis product. Glucose as control (1), rice straw by commercial cellulase (2), rice straw by cellulase obtained from Schizophyllum commune NAIMCC-F-03379 (3), rice straw by xylanase obtained from Aspergillus oryzae (4), rice straw by combination of cellulase and xylanase (5), wheat bran by commercial cellulase (6), wheat bran by cellulase obtained from Schizophyllum commune NAIMCC-F-03379 (7), wheat bran by xylanase obtained from Aspergillus oryzae (8) and wheat bran by combination of cellulase and xylanase (9) on TLC plates

Thermodependence of the activity of the various cellulases. (a) pNPC used as substrate: closed circles, full-length cold-adapted cellulase, Cel5G; hexagons, CM of the cold-adapted cellulase, Cel5G_CM; closed triangles, mesophilic cellulase from Erwinia chrysanthemi, Cel5A. (b) Thermodependence of the cold-adapted cellulases using CMC as substrate: closed circles, full-length cold-adapted cellulase, Cel5G; open circles, mutant Cel5GΔ1P; closed squares, mutant Cel5GΔ2P; open squares, mutant Cel5GΔ2PmutCC; hexagons, Cel5G_CM.

The hydrolysis was conducted at 50°C for 24 h (a), 36 h (b) and 48 h (c), respectively. Points and lines represented the mean values of four replicates and calculated values based on logarithmic equation (), respectively. X is cellulase dosage (FPU/g). Y is RS yield. The estimated values of α, Y₀ and determination coefficient (R²) under specific conditions were listed as follows: Cellulase alone for 24 h, α = 116.26, Y₀ = 287.92, R² = 0.9775; Optimized cellulase and EXLX1 mixture for 24 h, α = 128.58, Y₀ = 471.14, R² = 0.9033; Cellulase alone for 36 h, α = 122.02, Y₀ = 391.46, R² = 0.9612; Optimized cellulase and EXLX1 mixture for 36 h, 110.10, Y₀ = 524.92, R² = 0.9385; Cellulase alone for 48 h, α = 132.54, Y₀ = 414.45, R² = 0.9813; Optimized cellulase and EXLX1 mixture for 48 h: α = 114.64, Y₀ = 555.74, R² = 0.9405.