PMC

OCT images after suturing in Pair 1 – Pair 6 (A–F). The central scans and side scans were montaged. We made measurements of the graft and host thickness at the center. A: In Pair 1, graft and host thickness were 358 μm and 111 μm, respectively. At the suture zone, there was anterior bulging and anterior surface mismatch (arrows), and posterior bulging (arrow heads). B: In Pair 2, graft and host thickness were 328 μm and 235 μm, respectively. The graft fit into the host side pocket with less bulging at the suture zone. C: In Pair 3, graft and host thickness were 252μm and 200μm, respectively. At the suture zone, there was anterior bulging and anterior surface mismatch (arrows), and posterior bulging (arrow heads). D: In Pair 4, graft and host thickness were 295 μm and 234 μm, respectively. The graft fit into the host side pocket with less bulging at the suture zone. E: In Pair 5, graft and host thickness were 348 μm and 224 μm, respectively. The graft fit into the host side pocket with less bulging at the suture zone. F: In Pair 6, graft and host thickness were 174 μm and 208 μm, respectively. The graft fits into the host side pocket with less bulging at the suture zone.

(A) Scatter and histogram plots of bulge formation time (τ_B) and cracking angle (Δθ) reveal three different bulging patterns in response to cell wall damage by cephalexin: fast bulging without cracking (b, red dot, τ_B<10⁻² min), slow bulging without cracking (c, green dot, τ_B>10⁻² min), and slow bulging with cracking (d, blue dot, τ_B>10⁻² min). Yellow dots and bar graphs represent slow bulging cells (top and middle panels). Gray bars represent all isogenic cells examined (N=195, right panel). (B–D) Representative bulge depth measurement and snapshots of cells before (t₂) and after (t₃) bulge formation for each bulging pattern. Magenta lines represent measurement of cracking angles. All scale bars represent 2 μm. See also and for cell 6, cell 75 and cell 123.

(a) Schematic diagram of measurement regions; (b) load-depth curves of unformed regions; (c) load-depth curves of different regions of the bulging sample without LPS; (d) load-depth curves of different regions of the bulging sample with LPS; (e) load-depth curves of bulging central regions.