PMC

a Blood film of cells exposed to 1:1, THC:CBD extract. Blood film showing red cell changes in 1:1 THC:CBD extract—marked red cell agglutination with red cell degeneration. Scale = 100 μm. b Blood film of cells exposed to 4:1, THC:CBD extract. Blood film showing red cell changes in 4:1 THC:CBD extract—marked red cell degeneration; hardly any intact RBCs on film. Scale = 100 μm

 Photomicrographs of blood films submitted for examination for the presence of vacuolated lymphocytes. (A) Routine May-Grunwald-Giemsa stained blood film showing two lymphocytes with many large bold vacuoles, such as are seen in GM1 gangliosidosis, and juvenile Batten’s disease. (B) Routine May-Grunwald-Giemsa stained blood film showing a lymphocyte with discrete small vacuoles seen in Pompe’s disease and adult acid maltase deficiency. (C) Periodic acid Schiff (PAS) stained blood film showing a lymphocyte with PAS positive inclusions in Pompe’s disease and adult acid maltase deficiency. (D) Toluidine blue stained blood film showing metachromatic cytoplasmic inclusions in a lymphocyte. (E) Enzyme histochemical demonstration of β galactosidase in a blood film showing normal enzyme activity in a lymphocyte and eosinophil but negative activity in a neutrophil. (F) Enzyme histochemical demonstration of β galactosidase in a blood film showing absent enzyme activity in a lymphocyte and a granulocyte seen in GM1 gangliosidosis and galactosialidosis.

(A and B) SEM of film formed at the air-liquid interface in whole blood and plasma clots. At places where the film is torn (due to SEM sample processing procedures), red blood cells (asterisk), platelets (thick arrow), and fibrin (thin arrow) are observed in whole blood clots (A), and fibrin (asterisk) in plasma clots (B). Red blood cells are also visible through the film in whole blood clots. (C and D) LSCM of film formed at the air-liquid interface in whole blood and plasma clots. Fibrinogen was fluorescently labeled with Alexa Fluor 488 (green) or Alexa Fluor 594 (red). Under fully hydrated conditions of LSCM, tears in the film were not observed. (E) LSCM of a single Z-plane slice of a plasma clot showing continuous film around the clot. The central gap in the clot image is where the pipette was introduced into the plasma drop to inject thrombin. Images represent findings reproduced in at least n = 3 experiments. Scale bars: A and B, 10 μm; C and D, 50 μm; E, 1 mm. All images are representative of n = 3 experiments.

a Blood film of cells exposed to 100 ng/ml cannabidiol (CBD). Blood film showing red cell changes in 100 ng/ml CBD: red cell membrane distortion/indentation with occasional red cell fragments. Scale = 100 μm. b Blood film of cells exposed to 100 ng/ml delta-9-tetrahydrocannabinol (THC). Blood film showing red cell changes in 100 ng/ml THC: red cell fragmentation, burr cells. Scale = 100 μm