PMC

mRNA expression of ERα in MCF-7, ZR-75 and MCF-7/TAM Res cells treated for 24h with increasing concentrations of bergapten (B) as indicated. MCF-7/Tam Res were also treated with bergapten alone or in combination with OH-Tamoxifen. The housekeeping gene 36B4 was determined as control.

Bergapten increases gata1 and pu.1 in cdx4-mutant embryos. All embryos are displayed with anterior to the left and posterior to the right. (A) cdx4 heterozygous adults were incrossed, and the resulting embryos were treated with varying doses of bergapten. Bergapten rescues gata1 expression (level 3) in the cdx4-mutant embryos, and this rescue is most effective at 33 μM. Whole-mount embryos at the 10-somite stage are displayed from a representative experiment. (B) cdx4 heterozygous adults were incrossed, and the resulting embryos were treated with DMSO vehicle control, 20 μM DEAB, or 30 μM bergapten. Flatmounted embryos at the 10-somite stage are shown. WT embryos and cdx4 mutants stained with pu.1 were confirmed by genotyping. Bergapten does not change the expression pattern of mesodermal genes fli1, scl, and pu.1 in WT embryos. In cdx4-mutant embryos, however, pu.1 expression is rescued in the posterior lateral mesoderm when either DEAB or bergapten is applied. Ber, bergapten. Color images available online at www.liebertonline.com/zeb.

Effects of subchronic administration of bergapten (BG) on scopolamine-induced impairment of memory acquisition trial (A) and consolidation trial (B) using the PA test in mice. Bergapten (12.5 mg/kg, i.p.) was administered 6 days twice daily. On the seventh day bergapten was administered 30 min and scopolamine (SCOP, 1 mg/kg, i.p.) 20 min before the first trial (A) or bergapten was administered immediately after first trial and scopolamine 10 min after bergapten injection (B) and animals were re-tested 24 h after the last injection; n = 8–12; the means ± SEM; *p < 0.05; ***p < 0.01 vs. saline-treated control group, ^##p < 0.01 vs. scopolamine-treated control group; ^^^p < 0.001 vs. bergapten-treated (12.5 mg/kg) group; Bonferroni’s test.

Structures of psoralen and bergapten