|
| Status |
Public on Dec 08, 2023 |
| Title |
ALDH9A1B +/+ +tt-DDE4 |
| Sample type |
SRA |
| |
|
| Source name |
Tg(fli1:EGFP)
|
| Organism |
Danio rerio |
| Characteristics |
tissue: larvae
treatment: yes, treated with tt-DDE
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol method
RNA libraries were prepared for sequencing using standard BGISeq-500 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
aldh9a1b_DDE_4
|
| Data processing |
QC with fastqc nersion 0.11.9 Parameters:fastqc *.fastq
trimmed with trim_galore version 0.6.7. Parameters: trim_galore phred33 -q 30 --length 36 --stringency 3
alignment with hisat2 version 2.2.1 . Parameters: hisat2 -t -p 30 -x
Get raw counts with featureCounts Version 2.0.1. Parameters: featureCounts -T 5 -p -t exon -g gene_id -a
Assembly: GRCz11
Supplementary files format and content: Raw counts generated with featureCounts Version 2.0.1.
|
| |
|
| Submission date |
Jan 20, 2023 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Xin Qian |
| E-mail(s) |
Xin.Qian@medma.uni-heidelberg.de
|
| Phone |
015257444806
|
| Organization name |
Medical Faculty Mannheim, Heidelberg University
|
| Department |
Vascular Biology&Tumor Angiogenesis
|
| Street address |
Ludolf-Krehl-Str. 13-17
|
| City |
Mannheim |
| State/province |
Baden-Wuerttemberg |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24776 |
| Series (1) |
| GSE223340 |
The function and mechanism of aldh9a1b in diabetes |
|
| Relations |
| BioSample |
SAMN32811382 |
| SRA |
SRX19099981 |
