|
| Status |
Public on Feb 03, 2011 |
| Title |
Spleen, day 10, fish 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Spleen of infected salmon
|
| Organism |
Salmo salar |
| Characteristics |
disease state: challenged with L. salmonis
|
| Treatment protocol |
Salmon louse of LsGulen strain were reared in the hatchery and egg strings were collected and placed into an incubator until they reached the copepodid stage. Atlantic salmon were challenged with L. salmonis (approximately 100 copepodids per fish).
|
| Growth protocol |
Salmon (100-200 g) was kep in tanks with sea water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the iPrep™ TRIzol® Plus kit (Invitrogen), and purified with RNeasy mini kit (QIAGEN)
|
| Label |
Cy5
|
| Label protocol |
Amplified and labeled according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Channel 2 |
| Source name |
Spleen of control salmon
|
| Organism |
Salmo salar |
| Characteristics |
disease state: none
|
| Treatment protocol |
Salmon louse of LsGulen strain were reared in the hatchery and egg strings were collected and placed into an incubator until they reached the copepodid stage. Atlantic salmon were challenged with L. salmonis (approximately 100 copepodids per fish).
|
| Growth protocol |
Salmon (100-200 g) was kep in tanks with sea water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the iPrep™ TRIzol® Plus kit (Invitrogen), and purified with RNeasy mini kit (QIAGEN)
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| |
| Hybridization protocol |
Hybridized according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Arrays were scanned GenePix 4100A at a resolution of 5 microns
|
| Description |
Time-point 4 of 5, biological replicate 4 of 4
|
| Data processing |
GenePix Pro 6.0 was used for spot to grid alignment, feature extraction and quantification. After removal of flagged spots lowess normalization was performed
|
| |
|
| Submission date |
Jan 31, 2011 |
| Last update date |
Feb 03, 2011 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
|
| Phone |
+47 97602165
|
| Organization name |
Nofima AS
|
| Department |
Fish Health
|
| Street address |
Osloveien 1
|
| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10706 |
| Series (2) |
| GSE26984 |
Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in spleen of Atlantic salmon |
| GSE27013 |
Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in Atlantic salmon |
|
