|
| Status |
Public on Apr 01, 2010 |
| Title |
FRL Hippocampus Cohort 1, biological rep11 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus from Flinders Depression Resistent Line (FRL) rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: FRL
brain region: Hippocampus
cohort: Cohort 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from Hippocampus of Flinders Depression Resistant line (FRL) rats
|
| Data processing |
The data was analysed with Rosetta Resolver version 5.0
|
| |
|
| Submission date |
Feb 17, 2010 |
| Last update date |
Feb 18, 2010 |
| Contact name |
Adam Taylor |
| Organization name |
GalxoSmithKline
|
| Street address |
Medicine Research Center
|
| City |
Stevenage |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE20388 |
Expression Profiling of a Genetic Animal Model of Depression |
|
