|
| Status |
Public on May 17, 2010 |
| Title |
muscle_postTZD_postClamp_IRd_s51 |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle (vastus lateralis)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: muscle
tzd treatment: postTZD
clamp status: postClamp
insulin resistance: IRd (insulin resistant)
subject: 51
|
| Treatment protocol |
post-TZD, post-clamp, insulin resistant, , subject 51
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were isolated and processed as recommended by Affymetrix, Inc. (Affymetrix GeneChip® Expression Analysis Technical Manual) using TRIzol Reagent (Invitrogen Corp., Carlsbad, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix GeneChip® IVT Labeling Kit
|
| |
|
| Hybridization protocol |
15 ug of biotin-tagged cRNA was fragmented following prescribed protocols (Affymetrix GeneChip® Expression Analysis Technical Manual). 10 ug of fragmented target cRNA was hybridized (45°C with rotation for 16 hr, Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix Human Genome U133 Plus 2.0 array. The GeneChip® arrays were washed and stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
| Description |
no additional information
|
| Data processing |
Data analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method
|
| |
|
| Submission date |
Nov 13, 2008 |
| Last update date |
May 17, 2010 |
| Contact name |
Gene Hsiao |
| E-mail(s) |
gene@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13070 |
Human Insulin Resistance and Thiazolidinedione-Mediated Insulin Sensitization |
|
