|
| Status |
Public on Feb 25, 2019 |
| Title |
MYb71_ad7_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
whole body
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2/Wide type
treatment: MYb71
time point: adults (7 days)
medium: mNGM
temperature: 20C
|
| Treatment protocol |
Worms were fed to OP50 and Ochrobactrum at the different stage (from L2 to adults)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
F14878
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-quality sequence, then mapped to WS235 whole genome using STAR-2.5.3a
Read counts per gene is extracted via STAR-2.5.3a
Genome_build: WS235
Supplementary_files_format_and_content: tab-delimited text files include Raw reads counts for each Sample ...
|
| |
|
| Submission date |
Mar 02, 2018 |
| Last update date |
Feb 25, 2019 |
| Contact name |
Hinrich Schulenburg |
| E-mail(s) |
hschulenburg@zoologie.uni-kiel.de
|
| Organization name |
Christian-Albrechts-Universitaet zu Kiel
|
| Department |
Evolutionary Ecology and Genetics, Zoological Institute
|
| Lab |
Schulenburg Group
|
| Street address |
Am Botanischen Garten 1-9
|
| City |
Kiel |
| ZIP/Postal code |
24118 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13657 |
| Series (1) |
| GSE111364 |
Transcriptome of microbiome for the nematode Caenorhabditis elegans |
|
| Relations |
| BioSample |
SAMN08630731 |
| SRA |
SRX3757982 |
