|
Status |
Public on Mar 09, 2009 |
Title |
N8642A2: Invasive ductal carcinoma (IDC) |
Sample type |
RNA |
|
|
Source name |
frozen breast tissue
|
Organism |
Homo sapiens |
Characteristics |
Invasive ductal carcinoma (IDC)
|
Treatment protocol |
The tissues were embedded in Tissue-TekĀ® O.C.T., 5-um sections cut. The sections were stained using a standard H&E protocol, and tissue boundaries marked by a pathologist Using the marked slide as a map, tissues were microdissected. Adipose tissues were trimmed, the tumor and normal tissues were separated and stored in liquid nitrogen.
|
Growth protocol |
Breast tissues (normal and malignant) from patients that underwent mastectomy for various stages of breast carcinoma were collected and frozen in liquid nitrogen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from microdissected breast tissues using the Trizol method.
|
Label |
biotin
|
Label protocol |
Labeling was done according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Hybridization was carried out according to the standard Affymetrix protocol
|
Scan protocol |
According to the Affymetrix standard protocol. (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array)
|
Description |
frozen breast tissue
|
Data processing |
Expression values were calculated using the robust multi-array average (RMA) algorithm
|
|
|
Submission date |
Mar 11, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
Dung-Tsa Chen |
E-mail(s) |
dung-tsa.chen@moffitt.org
|
Organization name |
Moffitt Cancer Center
|
Department |
Biostatistics and Bioinformatics
|
Street address |
12902 Magnolia Drive
|
City |
Tampa |
State/province |
FL |
ZIP/Postal code |
33647 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE10780 |
Proliferative genes dominate malignancy-risk gene signature in histologically-normal breast tissue |
|
Relations |
Reanalyzed by |
GSE119087 |