|
| Status |
Public on Jul 11, 2013 |
| Title |
WS iPSC from AG06300 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WS iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 37
genotype: WT
cell type: Werner Syndrome iPSC
|
| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=AG06300
|
| Treatment protocol |
NA
|
| Growth protocol |
Fibroblast culture was maintained in alpha MEM medium containing 15% FBS. iPSC/hESC culture was maintained on Matrigel with mTeSR1 medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol and purified by Direct-zol RNA MiniPrep (Zymo Research). RNA integrity was assessed by BioAnalyzer.
|
| Label |
biotin
|
| Label protocol |
cDNA was labeled by biotin using the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
cDNA was hybridization at 45C overnight. Wash and staining was performed by GeneChip Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
Arrays were scanned by GeneChip Scanner 3000 7G controlled AGCC Scan Control (Affymetrix)
|
| Description |
Gene expression data from WS iPSC culture
|
| Data processing |
Microarray data were analyzed by Partek Genomics Suite 6.6 with quantile normalization and RMA background correction. A batch effect due to scan date was removed before One-way ANOVA analysis for differentially expressed genes between normal and WS fibroblasts.
|
| |
|
| Submission date |
Jul 10, 2013 |
| Last update date |
Aug 13, 2013 |
| Contact name |
Hoi Hung Cheung |
| E-mail(s) |
cheungho@mail.nih.gov
|
| Phone |
301-451-8368
|
| Organization name |
NICHD
|
| Lab |
Laboratory of Clinical and Developmental Genomics
|
| Street address |
49 Convent Dr, Rm.2C08 Bldg.49
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE48761 |
Expression profiling of skin fibroblast and iPSC from Werner Syndrome |
|
