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Series GSE762

Query DataSets for GSE762

Status

Public on Jun 01, 2004

Title

CCFAlmasan_CaP1

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

The experiment design was multifold, consisting of a time course experiment on LNCaP C4-2 human prostate adenocarcinoma cells following irradiation to a dose of 10 Sv from a Cesium-137 mixed gamma and beta source, with two additional samples, one exposed to radiation in four fractions instead of all at once, and one containing the parent LNCaP cell line. PolyA+ RNA was extracted, processed and hybridized to the Affymetrix (Santa Clara, CA, www.affymetrix.com) HGU-95 (Av1-E) chip sets, and then washed, stained and scanned according to Affymetrix's protocols contained in the GeneChip(R) Expression Analysis Manual. Transcript abundance data from the scans was processed initially with Affymetrix' Microarray Suite 5(R), then by Silicon Genetics' (Redwood City, CA, www.silicongenetics.com) GeneSpring(R).
Keywords = Human
Keywords = Prostate
Keywords = Cancer
Keywords = Adenocarcinoma
Keywords = Ionizing Radiation
Keywords = Microarray
Keywords: ordered

Contributor(s)

Buchsbaum JC, Gong B, Hissong JG, Ray S, Klein EA, Heston WE, Almasan A

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Submission date

Oct 20, 2003

Last update date

Jul 08, 2016

Contact name

John Gilmary Hissong

E-mail(s)

hissonj@ccf.org