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| Status |
Public on Jul 15, 2015 |
| Title |
HEK293T cells treated with a G9a small molecule inhibitor (UNC0638) or vehicle (DMSO), WIZ siRNA, G9a siRNA, or scrambled (control) siRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of HEK293T cells following treatment with a small molecule inhibitor of catalytic activity of the G9a lysine methyltransferase (UNC0638, 1 micromolar, 48 hours), or siRNA against WIZ or G9a (72 hours treatment).
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| |
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| Overall design |
Two condition experiment, UNC0638 vs DMSO, G9a siRNA vs control siRNA, or WIZ siRNA vs control siRNA. Biological replicates: UNC0638 vs DMSO 5 biological replicates with 2 dye swap experiments; G9a siRNA vs control siRNA, 2 biological replicates; WIZ siRNA vs control siRNA, 2 biological replicates with 2 dye swap experiments.
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| |
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| Contributor(s) |
Pattenden SG, Parker JS |
| Citation(s) |
26338712 |
| Submission date |
Jul 14, 2015 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Sam Pattenden |
| E-mail(s) |
pattenden@unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Center for Integrative Chemical Biology & Drug Discovery
|
| Street address |
3015 Marsico Hall 125 Mason Farm Road CB# 7363
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7363 |
| Country |
USA |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA289912 |
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