Methylation profiling by high throughput sequencing
Summary
The DNA methylome of 42 primary neuroblastoma tumors is profiled by enrichment with a methyl-CpG-binding domain (MBD) and massively parallel sequencing
Overall design
DNA of 42 primary tumors is sheared (fragments of ± 200 bp), followed by MBD-based (MethylCap kit of Diagenode) enrichement, library preparation and multiplexing. Captured DNA fractions were sequenced paired-end on Illumina Genome Analyzer IIx.
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